Effect of HIV-1 Tat on Secretion of TNF-α and IL-1β by U87 Cells in AIDS Patients with or without AIDS Dementia Complex

Key words: 
• HIV-1tat gene /
• AIDSdementia complex /
• Cytokines /
• TNF-α /
• IL-1β /
• Neurotoxicity

Abstract: ObjectiveToexplore the role of HIV-1 tat gene variations in AIDSdementia complex(ADC) pathogenesis.
MethodsHIV-1tat genes derived from peripheral spleen and central basal ganglia of anAIDSpatient with ADC and anAIDSpatientwithoutADC were cloned for sequence analysis. HIV-1 tat genesequence alignmentwasperformed by using CLUSTAL W andthephylogentic analysiswas conductedbyusing Neighbor-joining with MEGA4 software.All tat genes wereused to construct recombinant retroviral expressing vector MSCV-IRES-GFP/tat. The MSCV-IRES-GFP/tat was cotransfected into 293T cells with pCMV-VSV-G and pUMVC vectorsto assemble the recombinant retrovirus. After infection of gliomas U87 cells with equal amount of the recombinant retrovirus, TNF-α, and IL-1β concentrations inthe supernatant of U87 cells were determined with ELISA.
ResultsHIV-1tat genes derived from peripheral spleen and central basal ganglia ofthe AIDS patient with ADC andtheother onewithoutADCexhibited genetic variations.Tat variations and amino acid mutation sites existed mainly at Tat protein core functional area (38-47aa). All Tat proteinscould induce U87 cells to produce TNF-α and IL-1β, but thelevel of IL-1β production was different among Tatproteins derived fromtheADC patient’s spleen, basal ganglia, andthenon-ADC patient’s spleen.The level ofTat proteinsderived fromtheADC patient’s spleen,basal ganglia, andthenon-ADC patient’sspleen were obviously higher thanthat fromthe non-ADC patient’s basal ganglia.
ConclusionTat protein core functional area (38-47aa) mayserve as the key area of enhancing the secretion of IL-1β.This may be related with the neurotoxicity of HIV-1 Tat.
ObjectiveToexplore the role of HIV-1 tat gene variations in AIDSdementia complex(ADC) pathogenesis.
MethodsHIV-1tat genes derived from peripheral spleen and central basal ganglia of anAIDSpatient with ADC and anAIDSpatientwithoutADC were cloned for sequence analysis. HIV-1 tat genesequence alignmentwasperformed by using CLUSTAL W andthephylogentic analysiswas conductedbyusing Neighbor-joining with MEGA4 software.All tat genes wereused to construct recombinant retroviral expressing vector MSCV-IRES-GFP/tat. The MSCV-IRES-GFP/tat was cotransfected into 293T cells with pCMV-VSV-G and pUMVC vectorsto assemble the recombinant retrovirus. After infection of gliomas U87 cells with equal amount of the recombinant retrovirus, TNF-α, and IL-1β concentrations inthe supernatant of U87 cells were determined with ELISA.
ResultsHIV-1tat genes derived from peripheral spleen and central basal ganglia ofthe AIDS patient with ADC andtheother onewithoutADCexhibited genetic variations.Tat variations and amino acid mutation sites existed mainly at Tat protein core functional area (38-47aa). All Tat proteinscould induce U87 cells to produce TNF-α and IL-1β, but thelevel of IL-1β production was different among Tatproteins derived fromtheADC patient’s spleen, basal ganglia, andthenon-ADC patient’s spleen.The level ofTat proteinsderived fromtheADC patient’s spleen,basal ganglia, andthenon-ADC patient’sspleen were obviously higher thanthat fromthe non-ADC patient’s basal ganglia.
ConclusionTat protein core functional area (38-47aa) mayserve as the key area of enhancing the secretion of IL-1β.This may be related with the neurotoxicity of HIV-1 Tat.