Volume 27 Issue 10
Oct.  2014
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BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long. H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China[J]. Biomedical and Environmental Sciences, 2014, 27(10): 763-769. doi: 10.3967/bes2014.111
Citation: BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long. H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China[J]. Biomedical and Environmental Sciences, 2014, 27(10): 763-769. doi: 10.3967/bes2014.111

H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

doi: 10.3967/bes2014.111
Funds:  This study was supported by the National High Technology Research and Development Program of China (863 Program) SQ2009AA02XK1487370
  • ObjectiveTo prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China. MethodsRecombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses. ResultsThe 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chickenembryo survival and trypsin-dependent characteristics. ConclusionThe 4 recombinantviruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China

doi: 10.3967/bes2014.111
Funds:  This study was supported by the National High Technology Research and Development Program of China (863 Program) SQ2009AA02XK1487370

Abstract: ObjectiveTo prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China. MethodsRecombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses. ResultsThe 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chickenembryo survival and trypsin-dependent characteristics. ConclusionThe 4 recombinantviruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines.

BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long. H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China[J]. Biomedical and Environmental Sciences, 2014, 27(10): 763-769. doi: 10.3967/bes2014.111
Citation: BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, SHU Yue Long. H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China[J]. Biomedical and Environmental Sciences, 2014, 27(10): 763-769. doi: 10.3967/bes2014.111

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