Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes
Abstract: ObjectivePoisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants isinefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. MethodsSeventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled andthree DNA barcodes (matK,rbcL, and ITS) were amplified, sequenced and tested.Three methods, Blast,pairwise global alignment (PWG)distance, and Tree-Building were tested for discrimination power. ResultsThe primer universality of all the three markers was high. Except in the case of ITS for Hemerocallisminor, the three barcodes were successfully generated from all the selected species. Among the three methodsapplied, Blast showed the lowest discrimination rate,whereasPWGDistance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method. ConclusionDNA barcoding technique provides us a fast tool for clinical identification of poisonous plants in China.We suggestmatK,rbcL, ITS used in combination as DNA barcodes for authentication of poisonous plants.
|XIE Lei, WANG YingWei, GUAN ShanYue, XIE LiJing, LONG Xin, SUN ChengYe. Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes[J]. Biomedical and Environmental Sciences, 2014, 27(10): 794-806. doi: 10.3967/bes2014.115