Volume 28 Issue 3
Mar.  2015
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SHI Zhong Fang, ZHAO Wei Jiang, XU Li Xin, DONG Li Ping, YANG Shao Hua, YUAN Fang. Downregulation of Aquaporin 4 Expression through Extracellular Signal-regulated Kinases1/2 Activation in Cultured Astrocytes Following Scratch-injury[J]. Biomedical and Environmental Sciences, 2015, 28(3): 199-205. doi: 10.3967/bes2015.026
Citation: SHI Zhong Fang, ZHAO Wei Jiang, XU Li Xin, DONG Li Ping, YANG Shao Hua, YUAN Fang. Downregulation of Aquaporin 4 Expression through Extracellular Signal-regulated Kinases1/2 Activation in Cultured Astrocytes Following Scratch-injury[J]. Biomedical and Environmental Sciences, 2015, 28(3): 199-205. doi: 10.3967/bes2015.026

Downregulation of Aquaporin 4 Expression through Extracellular Signal-regulated Kinases1/2 Activation in Cultured Astrocytes Following Scratch-injury

doi: 10.3967/bes2015.026
Funds:  the National Natural Science Foundation of China, No.81271286 to YUAN Fang and No.81228009 to YANG Shao Hua
  • ObjectiveTo investigate the role of extracellular signal-regulated kinase1/2 (ERK1/2) pathway in the regulation of aquaporin 4 (AQP4) expression inculturedastrocytes after scratch-injury. MethodsThe scratch-injury model was produced in cultured astrocytes of rat by a 10-μL plastic pipette tip. The morphological changes of astrocytes and lactate dehydrogenase (LDH) leakages were observed to assess the degree of scratch-injury. AQP4 expressionwas detected by immunofluorescence staining and Western blot, and phosphorylated-ERK1/2 (p-ERK1/2) expression was determined by Western blot. To explore the effect of ERK1/2 pathway on AQP4 expression in scratch-injured astrocytes, 10 μmol/L U0126 (ERK1/2inhibitor) was incubated in the medium at 30 min before the scratch-injury in some groups. ResultsIncreases in LDH leakage were observed at 1, 12, and 24 h after scratch-injury, and AQP4 expression was reduced simultaneously. Decrease in AQP4 expressionwas associated with a significant increase in ERK1/2 activation. Furthermore, pretreatment with U0126 blocked both ERK1/2 activation and decrease in AQP4 expression induced by scratch-injury. ConclusionThese results indicate that ERK1/2 pathway down-regulates AQP4 expression in scratch-injured astrocytes, and ERK1/2 pathway might be a novel therapeutic target in reversing the effects of astrocytes that contribute to traumatic brain edema.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Downregulation of Aquaporin 4 Expression through Extracellular Signal-regulated Kinases1/2 Activation in Cultured Astrocytes Following Scratch-injury

doi: 10.3967/bes2015.026
Funds:  the National Natural Science Foundation of China, No.81271286 to YUAN Fang and No.81228009 to YANG Shao Hua

Abstract: ObjectiveTo investigate the role of extracellular signal-regulated kinase1/2 (ERK1/2) pathway in the regulation of aquaporin 4 (AQP4) expression inculturedastrocytes after scratch-injury. MethodsThe scratch-injury model was produced in cultured astrocytes of rat by a 10-μL plastic pipette tip. The morphological changes of astrocytes and lactate dehydrogenase (LDH) leakages were observed to assess the degree of scratch-injury. AQP4 expressionwas detected by immunofluorescence staining and Western blot, and phosphorylated-ERK1/2 (p-ERK1/2) expression was determined by Western blot. To explore the effect of ERK1/2 pathway on AQP4 expression in scratch-injured astrocytes, 10 μmol/L U0126 (ERK1/2inhibitor) was incubated in the medium at 30 min before the scratch-injury in some groups. ResultsIncreases in LDH leakage were observed at 1, 12, and 24 h after scratch-injury, and AQP4 expression was reduced simultaneously. Decrease in AQP4 expressionwas associated with a significant increase in ERK1/2 activation. Furthermore, pretreatment with U0126 blocked both ERK1/2 activation and decrease in AQP4 expression induced by scratch-injury. ConclusionThese results indicate that ERK1/2 pathway down-regulates AQP4 expression in scratch-injured astrocytes, and ERK1/2 pathway might be a novel therapeutic target in reversing the effects of astrocytes that contribute to traumatic brain edema.

SHI Zhong Fang, ZHAO Wei Jiang, XU Li Xin, DONG Li Ping, YANG Shao Hua, YUAN Fang. Downregulation of Aquaporin 4 Expression through Extracellular Signal-regulated Kinases1/2 Activation in Cultured Astrocytes Following Scratch-injury[J]. Biomedical and Environmental Sciences, 2015, 28(3): 199-205. doi: 10.3967/bes2015.026
Citation: SHI Zhong Fang, ZHAO Wei Jiang, XU Li Xin, DONG Li Ping, YANG Shao Hua, YUAN Fang. Downregulation of Aquaporin 4 Expression through Extracellular Signal-regulated Kinases1/2 Activation in Cultured Astrocytes Following Scratch-injury[J]. Biomedical and Environmental Sciences, 2015, 28(3): 199-205. doi: 10.3967/bes2015.026

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