Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China

LI Yan Ming; TONG Xun Liang; XU Hong Tao; JU Yang; CAI Meng; WANG Chen

doi:10.3967/bes2016.009

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Volume 29 Issue 2

Feb. 2016

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Article Navigation > Biomedical and Environmental Sciences > 2016 > 29(2): 85-90

LI Yan Ming, TONG Xun Liang, XU Hong Tao, JU Yang, CAI Meng, WANG Chen. Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 85-90. doi: 10.3967/bes2016.009

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LI Yan Ming, TONG Xun Liang, XU Hong Tao, JU Yang, CAI Meng, WANG Chen. Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 85-90. doi: 10.3967/bes2016.009

Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China

doi: 10.3967/bes2016.009

Department of Respiratory and Critical CareMedicine, Beijing Hospital, Beijing 100730, China
Department of Geriatrics, Beijing Hospital, Beijing 100730, China
Department of Laboratory Medicine, Beijing Hospital, Beijing 100730, China
Department of Hospital Infection Control and Management, Beijing Hospital, Beijing 100730, China
Department of Respiratory Medicine, Chinese-Japanese Friendship Hospital, Beijing 100029, China

Funds: the grant 2012BAI05B02 from National Key Technology Research and Development Program%China and grant 81400037 from the National Natural Science Foundation of China

Abstract

ObjectiveTo gain greater insight into the prevalence drug resistant profiles ofM. abscessus from a general hospital in Beijing, China. MethodsPartial gene sequencing of16S,hsp65,and rpoB were used to distinguish the species of NTM isolates. All strains identified asM. abscessus were further enrolled in the drug susceptibility testing by using broth microdilution method. ResultsWe found thatM. avium complex was the most frequent NTM organism, accounting for 54.1%(33/61) of all isolates. Behind MAC, the second most common organisms were M. abscessus (22 out of 61, 36.1%). Average rates of resistance were 4.5% for AMK, 9.1% for LZD, and 13.6% for CLA, respectively. In contrast, resistance to LEV (17/22, 77.3%), IMI (9/22, 40.9%), and SMX (10/22, 45.5%) was noted in more than 40% ofM. abscessusisolates. DNA sequencing revealed that all the CLA-resistant isolates harbored nucleotide substitutions in position 2058 (1/3, 33.3%) or 2059 (2/3, 66.7%) of 23S rRNA. ConclusionIn conclusion, our data demonstrated thatM. intracellulare andM. abscessuswere the most common NTM species in the general hospital of Beijing.CLA, AMK, LZD showed promising activity, whereas LEV, IMI, and SMX exhibited poor activity againstM. abscessus in vitro.
- Nontuberculous mycobacteria,
- Mycobacterium abscessus,
- China
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China

doi: 10.3967/bes2016.009

Department of Respiratory and Critical CareMedicine, Beijing Hospital, Beijing 100730, China

Department of Geriatrics, Beijing Hospital, Beijing 100730, China

Department of Laboratory Medicine, Beijing Hospital, Beijing 100730, China

Department of Hospital Infection Control and Management, Beijing Hospital, Beijing 100730, China

Department of Respiratory Medicine, Chinese-Japanese Friendship Hospital, Beijing 100029, China

Funds: the grant 2012BAI05B02 from National Key Technology Research and Development Program%China and grant 81400037 from the National Natural Science Foundation of China

Key words:

Abstract: ObjectiveTo gain greater insight into the prevalence drug resistant profiles ofM. abscessus from a general hospital in Beijing, China. MethodsPartial gene sequencing of16S,hsp65,and rpoB were used to distinguish the species of NTM isolates. All strains identified asM. abscessus were further enrolled in the drug susceptibility testing by using broth microdilution method. ResultsWe found thatM. avium complex was the most frequent NTM organism, accounting for 54.1%(33/61) of all isolates. Behind MAC, the second most common organisms were M. abscessus (22 out of 61, 36.1%). Average rates of resistance were 4.5% for AMK, 9.1% for LZD, and 13.6% for CLA, respectively. In contrast, resistance to LEV (17/22, 77.3%), IMI (9/22, 40.9%), and SMX (10/22, 45.5%) was noted in more than 40% ofM. abscessusisolates. DNA sequencing revealed that all the CLA-resistant isolates harbored nucleotide substitutions in position 2058 (1/3, 33.3%) or 2059 (2/3, 66.7%) of 23S rRNA. ConclusionIn conclusion, our data demonstrated thatM. intracellulare andM. abscessuswere the most common NTM species in the general hospital of Beijing.CLA, AMK, LZD showed promising activity, whereas LEV, IMI, and SMX exhibited poor activity againstM. abscessus in vitro.

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