Volume 29 Issue 2
Feb.  2016
Turn off MathJax
Article Contents

ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 127-136. doi: 10.3967/bes2016.014
Citation: ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 127-136. doi: 10.3967/bes2016.014

Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

doi: 10.3967/bes2016.014
Funds:  theXiamen Science and Technology Project of China(3502Z20123003)%Fundamental Research Funds for the Central Universities of China(2011121006)%National Undergraduate Training Program for Innovation and Entrepreneurship(201510384140)
  • ObjectiveCr(VI) removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr(VI) bioremediation from sediment samples and to optimize parameters of biodegradation. MethodsStrains with the ability to tolerate Cr(VI) were obtained by serial dilution and spread plate methods and characterized by morphology, 16S rDNA identification, and phylogenetic analysis. Cr(VI) was determined using the 1,5-diphenylcarbazide method, and the optimum pH and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. ResultsFifty-five strains were obtained, and one strain (Sporosarcina saromensisM52; patent application number: 201410819443.3) having the ability to tolerate 500 mg Cr(VI)/L wasselected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr(VI)/L in 24 h, achieving the highest removal efficiency at pH 7.0-8.5 and 35°C. Moreover, M52 could completely degrade 100 mg Cr(VI)/L at pH 8.0 and35 °C in 24 h. The mechanism involved in the reduction of Cr(VI) was considered to be bioreduction rather than absorption. ConclusionThe strong degradation ability ofS. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation ofheavy metal pollution.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1557) PDF downloads(73) Cited by()

Proportional views
Related

Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

doi: 10.3967/bes2016.014
Funds:  theXiamen Science and Technology Project of China(3502Z20123003)%Fundamental Research Funds for the Central Universities of China(2011121006)%National Undergraduate Training Program for Innovation and Entrepreneurship(201510384140)

Abstract: ObjectiveCr(VI) removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr(VI) bioremediation from sediment samples and to optimize parameters of biodegradation. MethodsStrains with the ability to tolerate Cr(VI) were obtained by serial dilution and spread plate methods and characterized by morphology, 16S rDNA identification, and phylogenetic analysis. Cr(VI) was determined using the 1,5-diphenylcarbazide method, and the optimum pH and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. ResultsFifty-five strains were obtained, and one strain (Sporosarcina saromensisM52; patent application number: 201410819443.3) having the ability to tolerate 500 mg Cr(VI)/L wasselected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr(VI)/L in 24 h, achieving the highest removal efficiency at pH 7.0-8.5 and 35°C. Moreover, M52 could completely degrade 100 mg Cr(VI)/L at pH 8.0 and35 °C in 24 h. The mechanism involved in the reduction of Cr(VI) was considered to be bioreduction rather than absorption. ConclusionThe strong degradation ability ofS. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation ofheavy metal pollution.

ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 127-136. doi: 10.3967/bes2016.014
Citation: ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 127-136. doi: 10.3967/bes2016.014

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return