Volume 36 Issue 8
Sep.  2023
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Method for Vitamin D Deficiency Screening[J]. Biomedical and Environmental Sciences, 2023, 36(8): 785-785. doi: 10.3967/bes2023.109
Citation: Method for Vitamin D Deficiency Screening[J]. Biomedical and Environmental Sciences, 2023, 36(8): 785-785. doi: 10.3967/bes2023.109

Method for Vitamin D Deficiency Screening

doi: 10.3967/bes2023.109
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Method for Vitamin D Deficiency Screening

doi: 10.3967/bes2023.109
Method for Vitamin D Deficiency Screening[J]. Biomedical and Environmental Sciences, 2023, 36(8): 785-785. doi: 10.3967/bes2023.109
Citation: Method for Vitamin D Deficiency Screening[J]. Biomedical and Environmental Sciences, 2023, 36(8): 785-785. doi: 10.3967/bes2023.109
  • This standard specifies the indicators, reference values, and measurement methods for vitamin D deficiency and insufficiency screening in the people.

    This standard is applicable to the determination of vitamin D nutritional status in the people.

  • The following documents are essential for the application of this document. For the references dated, only their dated versions are applies to this document; for the references undated, their latest versions, including all amendments, are applicable to this document.

    GB/T 603 Chemical reagent Preparation of formulations and products used in test methods

    GB/T 6682 Analysis of laboratory water specifications and test methods

    WS/T 225 Collection and processing of blood samples for clinical chemical testing

  • The following terms and definitions apply to this document.

  • The main circulating form of vitamin D in the blood, with good stability, is recognized as a reliable indicator for evaluating the nutritional status of vitamin D in the human body. It mainly includes two forms: 25 (OH) D2 and 25 (OH) D3, of which 25 (OH) D3 is the main form of vitamin D present in the blood.

  • Vitamin D deficiency can be determined when the content of 25-hydroxyvitamin D in human serum (or plasma) is lower than the reference values for deficiency.

  • Vitamin D insufficiency can be determined when the content of 25-hydroxyvitamin D in human serum (or plasma) is lower than the reference values for the normal people, but higher than that for deficiency.

  • Determination indicators and reference values for vitamin D nutritional status in the people are shown in Table 1.

    Determination indicator Normal Insufficiency Deficiency
    Content of 25-hydroxyvitamin D
    in serum (or plasma)
    ng/mL nmol/L ng/mL nmol/L ng/mL nmol/L
    ≥ 20 ≥ 50 ≥ 12 to < 20 ≥ 30 to < 50 < 12 < 30

    Table 1.  Determination indicators and reference values for vitamin D nutritional status in the people

  • Follow the methods specified in Appendix A.

  • Follow the methods specified in Appendix B.

  • Follow the methods specified in Appendix C.

  • Follow the methods specified in Appendix D.

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