Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

HAI-TAO WANG; BIN WANG; ZHI-JUN LIU; ZHI-QIANG BAI; LING LI; HAI-YAN LIU; DONG-MENG QIAN; ZHI-YONG YAN; XU-XIA SONG

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Volume 22 Issue 4

Aug. 2009

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Article Navigation > Biomedical and Environmental Sciences > 2009 > 22(4): 354-358

HAI-TAO WANG, BIN WANG, ZHI-JUN LIU, ZHI-QIANG BAI, LING LI, HAI-YAN LIU, DONG-MENG QIAN, ZHI-YONG YAN, XU-XIA SONG. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells[J]. Biomedical and Environmental Sciences, 2009, 22(4): 354-358.

Citation:

HAI-TAO WANG, BIN WANG, ZHI-JUN LIU, ZHI-QIANG BAI, LING LI, HAI-YAN LIU, DONG-MENG QIAN, ZHI-YONG YAN, XU-XIA SONG. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells[J]. Biomedical and Environmental Sciences, 2009, 22(4): 354-358.

Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

Department of Microbiology, Qingdao Medical College, and Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, Shandong, China;Qingdao Center for Disease Control and Prevention, Qingdao 266033, Shandong, China
Department of Microbiology, Qingdao Medical College, and Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, Shandong, China
LRB 270E, University of Massachusetts Medical School, Worcester, MA. 01605, USA

Funds: 国家自然科学基金(30770105)%Mt.Tat Scholar Construction Engineering Special Foundation of Shandong Province

Abstract

Objectives To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P<0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.
- Human cytomegalovirus,
- U251 cells,
- Nerve growth factor
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

Department of Microbiology, Qingdao Medical College, and Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, Shandong, China;Qingdao Center for Disease Control and Prevention, Qingdao 266033, Shandong, China

Department of Microbiology, Qingdao Medical College, and Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266071, Shandong, China

LRB 270E, University of Massachusetts Medical School, Worcester, MA. 01605, USA

Funds: 国家自然科学基金(30770105)%Mt.Tat Scholar Construction Engineering Special Foundation of Shandong Province

Key words:

Abstract: Objectives To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P<0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.

Citation:

HAI-TAO WANG, BIN WANG, ZHI-JUN LIU, ZHI-QIANG BAI, LING LI, HAI-YAN LIU, DONG-MENG QIAN, ZHI-YONG YAN, XU-XIA SONG. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells[J]. Biomedical and Environmental Sciences, 2009, 22(4): 354-358.