Volume 22 Issue 4
Aug.  2009
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HAI-TAO WANG, BIN WANG, ZHI-JUN LIU, ZHI-QIANG BAI, LING LI, HAI-YAN LIU, DONG-MENG QIAN, ZHI-YONG YAN, XU-XIA SONG. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells[J]. Biomedical and Environmental Sciences, 2009, 22(4): 354-358.
Citation: HAI-TAO WANG, BIN WANG, ZHI-JUN LIU, ZHI-QIANG BAI, LING LI, HAI-YAN LIU, DONG-MENG QIAN, ZHI-YONG YAN, XU-XIA SONG. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells[J]. Biomedical and Environmental Sciences, 2009, 22(4): 354-358.

Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

Funds:  国家自然科学基金(30770105)%Mt.Tat Scholar Construction Engineering Special Foundation of Shandong Province
  • Objectives To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P<0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

Funds:  国家自然科学基金(30770105)%Mt.Tat Scholar Construction Engineering Special Foundation of Shandong Province

Abstract: Objectives To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P<0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.

HAI-TAO WANG, BIN WANG, ZHI-JUN LIU, ZHI-QIANG BAI, LING LI, HAI-YAN LIU, DONG-MENG QIAN, ZHI-YONG YAN, XU-XIA SONG. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells[J]. Biomedical and Environmental Sciences, 2009, 22(4): 354-358.
Citation: HAI-TAO WANG, BIN WANG, ZHI-JUN LIU, ZHI-QIANG BAI, LING LI, HAI-YAN LIU, DONG-MENG QIAN, ZHI-YONG YAN, XU-XIA SONG. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells[J]. Biomedical and Environmental Sciences, 2009, 22(4): 354-358.

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