Volume 22 Issue 6
Dec.  2009
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CHEN-YU XU, YING GU, WANG-HENG HOU, Yu-QIONG QUE, SHUANG-GUAN GAO, TONG CHENG, NING-SHAO XIA. Tetracysteine as a Reporter for Gene Therapy[J]. Biomedical and Environmental Sciences, 2009, 22(6): 496-501.
Citation: CHEN-YU XU, YING GU, WANG-HENG HOU, Yu-QIONG QUE, SHUANG-GUAN GAO, TONG CHENG, NING-SHAO XIA. Tetracysteine as a Reporter for Gene Therapy[J]. Biomedical and Environmental Sciences, 2009, 22(6): 496-501.

Tetracysteine as a Reporter for Gene Therapy

  • Objective To study the feasibility of using tetracysteine (TC) reporter in gene therapy. Methods Effects of TC reporter and conventional reporter genes encoding green fluorescence protein (GFP) and luciferase (Luc) on expression and function of the therapeutic gene MGMT~(P140K) were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry (FCM). Results The TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection. Conclusion TC is a new kind of reporter gene for lentiviral vector in future gene therapy.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Tetracysteine as a Reporter for Gene Therapy

Abstract: Objective To study the feasibility of using tetracysteine (TC) reporter in gene therapy. Methods Effects of TC reporter and conventional reporter genes encoding green fluorescence protein (GFP) and luciferase (Luc) on expression and function of the therapeutic gene MGMT~(P140K) were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry (FCM). Results The TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection. Conclusion TC is a new kind of reporter gene for lentiviral vector in future gene therapy.

CHEN-YU XU, YING GU, WANG-HENG HOU, Yu-QIONG QUE, SHUANG-GUAN GAO, TONG CHENG, NING-SHAO XIA. Tetracysteine as a Reporter for Gene Therapy[J]. Biomedical and Environmental Sciences, 2009, 22(6): 496-501.
Citation: CHEN-YU XU, YING GU, WANG-HENG HOU, Yu-QIONG QUE, SHUANG-GUAN GAO, TONG CHENG, NING-SHAO XIA. Tetracysteine as a Reporter for Gene Therapy[J]. Biomedical and Environmental Sciences, 2009, 22(6): 496-501.

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