Effect of Nicotinamide on 12-O-tetradecanoyl-phorbol-13-acetate Exposed Mouse Skin Endonuclease Activity and DNA Synthesis

KRISHNA P.GUPTA; REENA RANI

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Volume 13 Issue 2

Jun. 2000

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Article Navigation > Biomedical and Environmental Sciences > 2000 > 13(2): 122-130

KRISHNA P.GUPTA, REENA RANI. Effect of Nicotinamide on 12-O-tetradecanoyl-phorbol-13-acetate Exposed Mouse Skin Endonuclease Activity and DNA Synthesis[J]. Biomedical and Environmental Sciences, 2000, 13(2): 122-130.

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KRISHNA P.GUPTA, REENA RANI. Effect of Nicotinamide on 12-O-tetradecanoyl-phorbol-13-acetate Exposed Mouse Skin Endonuclease Activity and DNA Synthesis[J]. Biomedical and Environmental Sciences, 2000, 13(2): 122-130.

Effect of Nicotinamide on 12-O-tetradecanoyl-phorbol-13-acetate Exposed Mouse Skin Endonuclease Activity and DNA Synthesis

Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Lucknow 226001, India

Abstract

Nicotinamide (NA), a relatively nontoxic compound, has been shown to inhibit tumor development, induce differentiation, increase the sensitization of the anticancer drug resistant cancer cells and is being used in different skin ailments. But there are not many reports on its mechanism of action. Here we report that NA induced endonuclease activity. This endonuclease induction by NA appeared to be dose dependent and a function of time. As evident by the use of modifiers of DNase I, this endonuclease appeared to be like DNase type I. Increased [3H] thymidine incorporation in DNA in the presence of NA is possibly a consequence of increased 3-OH'nicks due to increased DNA fragmentation by increased endonuclease activity. The present results would be of help in the better understanding of the mechanism of NA action and its improved use in cancer control.
- mechanism of action,
- DNA fragmentation,
- anticancer drug,
- used in
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effect of Nicotinamide on 12-O-tetradecanoyl-phorbol-13-acetate Exposed Mouse Skin Endonuclease Activity and DNA Synthesis

Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Lucknow 226001, India

Key words:

Abstract: Nicotinamide (NA), a relatively nontoxic compound, has been shown to inhibit tumor development, induce differentiation, increase the sensitization of the anticancer drug resistant cancer cells and is being used in different skin ailments. But there are not many reports on its mechanism of action. Here we report that NA induced endonuclease activity. This endonuclease induction by NA appeared to be dose dependent and a function of time. As evident by the use of modifiers of DNase I, this endonuclease appeared to be like DNase type I. Increased [3H] thymidine incorporation in DNA in the presence of NA is possibly a consequence of increased 3-OH'nicks due to increased DNA fragmentation by increased endonuclease activity. The present results would be of help in the better understanding of the mechanism of NA action and its improved use in cancer control.

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