Volume 14 Issue 3
Sep.  2001
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JI HONG-LONG. Thermal Diversities of Two Na+/H+ Exchanges in Guinea Pig Red Cells[J]. Biomedical and Environmental Sciences, 2001, 14(3): 171-179.
Citation: JI HONG-LONG. Thermal Diversities of Two Na+/H+ Exchanges in Guinea Pig Red Cells[J]. Biomedical and Environmental Sciences, 2001, 14(3): 171-179.

Thermal Diversities of Two Na+/H+ Exchanges in Guinea Pig Red Cells

  • Objective To test the effect of hypothermia on Na+/H+ exchange, activated by shrinkage and cytoplasmic acidosis. Method Amiloride-sensitive Na+ influx in guinea pig red cells was traced with isotope 22Na and intracellular Na+ concentration was measured by emission flame photometry. Result Amiloride-sensitive Na+ influx decreased linearly as a function of temperatures (about 37℃) in shrunken cells, but increased in acidified cells. The up-regulation of acid-induced Na+/H+ exchange by elevated temperature was enhanced by hypo-osmolarity. Less sensitivity of intracellular H+ site at 41℃ may be the mechanism for the inhibition of shrinkage-induced Na+/H+ exchange by elevated temperature. Heating-mediated explosive increase in the activity of acid-induced Na+/H+ exchange may be due to enhanced extracellular Na+ sensitivity and lower intracellular pH caused by acidic metabolites. Acid-induced Na+/H+ ewxchange contributes to cytoplasmic Na+ accumulation. Conclusion These two modes of Na+/H+ exchange with different response to elevated temperature may play different roles in the cellular pathogenesis of heatstroke.
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Thermal Diversities of Two Na+/H+ Exchanges in Guinea Pig Red Cells

Abstract: Objective To test the effect of hypothermia on Na+/H+ exchange, activated by shrinkage and cytoplasmic acidosis. Method Amiloride-sensitive Na+ influx in guinea pig red cells was traced with isotope 22Na and intracellular Na+ concentration was measured by emission flame photometry. Result Amiloride-sensitive Na+ influx decreased linearly as a function of temperatures (about 37℃) in shrunken cells, but increased in acidified cells. The up-regulation of acid-induced Na+/H+ exchange by elevated temperature was enhanced by hypo-osmolarity. Less sensitivity of intracellular H+ site at 41℃ may be the mechanism for the inhibition of shrinkage-induced Na+/H+ exchange by elevated temperature. Heating-mediated explosive increase in the activity of acid-induced Na+/H+ exchange may be due to enhanced extracellular Na+ sensitivity and lower intracellular pH caused by acidic metabolites. Acid-induced Na+/H+ ewxchange contributes to cytoplasmic Na+ accumulation. Conclusion These two modes of Na+/H+ exchange with different response to elevated temperature may play different roles in the cellular pathogenesis of heatstroke.

JI HONG-LONG. Thermal Diversities of Two Na+/H+ Exchanges in Guinea Pig Red Cells[J]. Biomedical and Environmental Sciences, 2001, 14(3): 171-179.
Citation: JI HONG-LONG. Thermal Diversities of Two Na+/H+ Exchanges in Guinea Pig Red Cells[J]. Biomedical and Environmental Sciences, 2001, 14(3): 171-179.

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