Induction of Apoptosis by Recombinant Soluble Human TRAIL in Jurkat Cells

GEN-HONG YAO; LI-JUN LING; JIAN-FENG LUAN; DONG YE; PEI-YUAN ZHU; QIAN-HONG LEI

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 20 Issue 6

Dec. 2007

Turn off MathJax

Article Contents

Article Navigation > Biomedical and Environmental Sciences > 2007 > 20(6): 470-477

GEN-HONG YAO, LI-JUN LING, JIAN-FENG LUAN, DONG YE, PEI-YUAN ZHU, QIAN-HONG LEI. Induction of Apoptosis by Recombinant Soluble Human TRAIL in Jurkat Cells[J]. Biomedical and Environmental Sciences, 2007, 20(6): 470-477.

Citation:

GEN-HONG YAO, LI-JUN LING, JIAN-FENG LUAN, DONG YE, PEI-YUAN ZHU, QIAN-HONG LEI. Induction of Apoptosis by Recombinant Soluble Human TRAIL in Jurkat Cells[J]. Biomedical and Environmental Sciences, 2007, 20(6): 470-477.

Induction of Apoptosis by Recombinant Soluble Human TRAIL in Jurkat Cells

Department of Transfusion, Jinling Hospital, Medical School, Nanjing University, Nanjing 210002, Jiangsu, China
Medical School, Nanjing University, Nanjing 210002, Jiangsu, China

Funds: Jiangsu Projects for Postdoctoral Research Funds(0601025B)%National Projects for Postdoctoral Research Funds(20060390940)

Abstract

Objective To investigate the therapeutic potential of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells. Methods Expression of TRAIL receptors (DR4 and DR5) was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation (DNA ladder) and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence. Results TRAIL inhibited the proliferation and induced internucleosomal DNA fragmentation (characteristic of apoptosis) and loss of mitochondrial membrane potential. Conclusion Recombinant soluble TRAIL can be used as a therapy for cancer.
- TRAIL,
- Apoptosis,
- Jurkat cells
References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views(1115) PDF downloads(28) Cited by()

Proportional views

Induction of Apoptosis by Recombinant Soluble Human TRAIL in Jurkat Cells

Department of Transfusion, Jinling Hospital, Medical School, Nanjing University, Nanjing 210002, Jiangsu, China

Medical School, Nanjing University, Nanjing 210002, Jiangsu, China

Funds: Jiangsu Projects for Postdoctoral Research Funds(0601025B)%National Projects for Postdoctoral Research Funds(20060390940)

Key words:

TRAIL /
Apoptosis /
Jurkat cells

Abstract: Objective To investigate the therapeutic potential of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells. Methods Expression of TRAIL receptors (DR4 and DR5) was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation (DNA ladder) and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence. Results TRAIL inhibited the proliferation and induced internucleosomal DNA fragmentation (characteristic of apoptosis) and loss of mitochondrial membrane potential. Conclusion Recombinant soluble TRAIL can be used as a therapy for cancer.

Citation: