Fenvalerate-induced Alterations in Calcium Homeostasis in Rat Ovary

JUN HE; JIAN-FENG CHEN; RU LIU; LIN SONG; HEBRON C. CHANG; XIN-RU WANG

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Volume 19 Issue 1

Feb. 2006

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Article Navigation > Biomedical and Environmental Sciences > 2006 > 19(1): 15-20

JUN HE, JIAN-FENG CHEN, RU LIU, LIN SONG, HEBRON C. CHANG, XIN-RU WANG. Fenvalerate-induced Alterations in Calcium Homeostasis in Rat Ovary[J]. Biomedical and Environmental Sciences, 2006, 19(1): 15-20.

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JUN HE, JIAN-FENG CHEN, RU LIU, LIN SONG, HEBRON C. CHANG, XIN-RU WANG. Fenvalerate-induced Alterations in Calcium Homeostasis in Rat Ovary[J]. Biomedical and Environmental Sciences, 2006, 19(1): 15-20.

Fenvalerate-induced Alterations in Calcium Homeostasis in Rat Ovary

Jiangsu Key Laboratory of Applied Toxicology, Nanjing Medical University,Nanjing 210029, Jiangsu, China

Funds: National Natural Science Foundation of China(C03010501)%the Preliminary Study of an Important Project in the National Basic Research(200150)%the Greatest Project in the National Basic Research(2002CB512908)

Abstract

Objective To observe the effects of fenvalerate on calcium homeostasis in rat ovary. Methods Female SpragueDawley rats were orally given fenvalerate at daily doses of 0.00, 1.91, 9.55, and 31.80 mg/kg for four weeks. The ovary ultrastucture was observed by electron microscopy. Serum free calcium concentration was measured by atomic absorption spectrophotometry. The activities of phosphorylase a in rat ovary were evaluated by the chromatometry. The total content of calmodulin in ovary was estimated by ELISA at each stage of estrous cycle. Radioimmunoassay (RIA) was used to evaluate the level of serum progesterone. Results Histopathologically, damages of ovarian corpus luteum cells were observed. An increase in serum free calcium concentration was observed in rats treated with 31.80mg/kg fenvalerate. The activities of phosphorylase a enhanced in all treated groups, and fenvalerate increased the total content of calmodulin significantly in estrus period. Serum progesterone levels declined in fenvalerate exposed rats in diestrus. Conclusion Fenvalerate interferes with calcium homeostasis in rat ovary. Also, the inhibitory effects of fenvalerate on serum progesterone levels may be mediated partly through calcium signals.
- Pyrethroids,
- Fenvalerate,
- Rat ovary,
- Calcium homeostasis,
- Progesterone
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Fenvalerate-induced Alterations in Calcium Homeostasis in Rat Ovary

Jiangsu Key Laboratory of Applied Toxicology, Nanjing Medical University,Nanjing 210029, Jiangsu, China

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Abstract: Objective To observe the effects of fenvalerate on calcium homeostasis in rat ovary. Methods Female SpragueDawley rats were orally given fenvalerate at daily doses of 0.00, 1.91, 9.55, and 31.80 mg/kg for four weeks. The ovary ultrastucture was observed by electron microscopy. Serum free calcium concentration was measured by atomic absorption spectrophotometry. The activities of phosphorylase a in rat ovary were evaluated by the chromatometry. The total content of calmodulin in ovary was estimated by ELISA at each stage of estrous cycle. Radioimmunoassay (RIA) was used to evaluate the level of serum progesterone. Results Histopathologically, damages of ovarian corpus luteum cells were observed. An increase in serum free calcium concentration was observed in rats treated with 31.80mg/kg fenvalerate. The activities of phosphorylase a enhanced in all treated groups, and fenvalerate increased the total content of calmodulin significantly in estrus period. Serum progesterone levels declined in fenvalerate exposed rats in diestrus. Conclusion Fenvalerate interferes with calcium homeostasis in rat ovary. Also, the inhibitory effects of fenvalerate on serum progesterone levels may be mediated partly through calcium signals.

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