Volume 16 Issue 2
Jun.  2003
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XUE-YUAN XIAO, YING TANG, XIU-PING WEI, DA-CHENG HE. A Preliminary Analysis of Non-small Cell Lung Cancer Biomarkers in Serum[J]. Biomedical and Environmental Sciences, 2003, 16(2): 140-148.
Citation: XUE-YUAN XIAO, YING TANG, XIU-PING WEI, DA-CHENG HE. A Preliminary Analysis of Non-small Cell Lung Cancer Biomarkers in Serum[J]. Biomedical and Environmental Sciences, 2003, 16(2): 140-148.

A Preliminary Analysis of Non-small Cell Lung Cancer Biomarkers in Serum

Funds:  This work was supported by Science Technology Key Project of Ministry of Education(Grant 272006)%国家重点基础研究发展计划(973计划)(Grant G1999053901)
  • Objective To identify potential serum biomarkers that could be used to discriminate lungcancers from normal. Methods Proteomic spectra of twenty-eight serum samples from patientswith non-small cell lung cancer and twelve from normal individuals were generated by SELDI(Surfaced Enhanced Laser Desorption/Ionization) Mass Spectrometry. Anion-exchange columns wereused to fractionate the sera into 6 designated pH groups. Two different types of protein chip arrays,IMAC-Cu and WCX2, were employed. Samples were examined in PBSII Protein Chip Reader(Ciphergen Biosystem Inc) and the discriminatory profiling between cancer and normal samples wasanalyzed with Biomarker Pattern software. Results Five distinct potential lung cancer biomarkerswith higher sensitivity and specificity were found, with four common biomarkers in both IMAC-Cuand WCX2 chip; the remaining biomarker occurred only in WCX2 chip. Two biomarkers wereup-regulated while three biomarkers were down-regulated in the serum samples from patients withnon-small cell lung cancer. The sensitivities provided by the individual biomarkers were 75%-96.43%and specificities were 75%-100%. Conclusions The preliminary results suggest that serum is acapable resource for detecting specific non-small cell lung cancer biomarkers. SELDI massspectrometry is a useful tool for the detection and identification of new potential biomarker ofnon-small cell lung cancer in serum.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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A Preliminary Analysis of Non-small Cell Lung Cancer Biomarkers in Serum

Funds:  This work was supported by Science Technology Key Project of Ministry of Education(Grant 272006)%国家重点基础研究发展计划(973计划)(Grant G1999053901)

Abstract: Objective To identify potential serum biomarkers that could be used to discriminate lungcancers from normal. Methods Proteomic spectra of twenty-eight serum samples from patientswith non-small cell lung cancer and twelve from normal individuals were generated by SELDI(Surfaced Enhanced Laser Desorption/Ionization) Mass Spectrometry. Anion-exchange columns wereused to fractionate the sera into 6 designated pH groups. Two different types of protein chip arrays,IMAC-Cu and WCX2, were employed. Samples were examined in PBSII Protein Chip Reader(Ciphergen Biosystem Inc) and the discriminatory profiling between cancer and normal samples wasanalyzed with Biomarker Pattern software. Results Five distinct potential lung cancer biomarkerswith higher sensitivity and specificity were found, with four common biomarkers in both IMAC-Cuand WCX2 chip; the remaining biomarker occurred only in WCX2 chip. Two biomarkers wereup-regulated while three biomarkers were down-regulated in the serum samples from patients withnon-small cell lung cancer. The sensitivities provided by the individual biomarkers were 75%-96.43%and specificities were 75%-100%. Conclusions The preliminary results suggest that serum is acapable resource for detecting specific non-small cell lung cancer biomarkers. SELDI massspectrometry is a useful tool for the detection and identification of new potential biomarker ofnon-small cell lung cancer in serum.

XUE-YUAN XIAO, YING TANG, XIU-PING WEI, DA-CHENG HE. A Preliminary Analysis of Non-small Cell Lung Cancer Biomarkers in Serum[J]. Biomedical and Environmental Sciences, 2003, 16(2): 140-148.
Citation: XUE-YUAN XIAO, YING TANG, XIU-PING WEI, DA-CHENG HE. A Preliminary Analysis of Non-small Cell Lung Cancer Biomarkers in Serum[J]. Biomedical and Environmental Sciences, 2003, 16(2): 140-148.

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