Volume 20 Issue 3
Jun.  2007
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RUI-JUAN GAO, YUE-XIN LIANG, DIAN-DONG LI, HONG-YIN ZHANG, YONG-SU ZHEN. Effect of Lidamycin on Telomerase Activity in Human Hepatoma BEL-7402 Cells[J]. Biomedical and Environmental Sciences, 2007, 20(3): 189-197.
Citation: RUI-JUAN GAO, YUE-XIN LIANG, DIAN-DONG LI, HONG-YIN ZHANG, YONG-SU ZHEN. Effect of Lidamycin on Telomerase Activity in Human Hepatoma BEL-7402 Cells[J]. Biomedical and Environmental Sciences, 2007, 20(3): 189-197.

Effect of Lidamycin on Telomerase Activity in Human Hepatoma BEL-7402 Cells

Funds:  国家高技术研究发展计划(863计划)(2006AA02A255)%国家自然科学基金(30472042%30300424)
  • Objective To investigate the effect of lidamycin(LDM)on telomerase activity in human hepatoma BEL-7402 cells under the condition of LDM inducing mitotic cell death and senescence.Methods Chromatin condensation was detected by co-staining with Hoechst 33342 and PI.Cell multinucleation was observed by Giemsa staining and genomic DNA was separated by agarose gel electrophoresis.Fluorescent intensity of Rho123 Was determined for mitochondrial membrane potential.MTT assay and SA-β-gal staining were employed to analyze the senescence-like phenotype.The expression of proteins was analyzed by Western blot.Telomerase activity was assayed by telomerase PCR-ELISA.Results Mitotic cell death occurred in LDM-treated cells characterized by unique and atypical chromatin condensation,multinucleation and increased mitochondrial membrane potential.However,no apoptotic bodies or DNA ladders were found.In addition,apoptosis-related proteins remained nearly unaltered.Senescence-like phenotype was identified by increased and elongated size of cells,growth retardation,enhanced SA-β-gal activity and the changes of senescence-related protein expression.Telomerase activity markedly decreased (P<0.01)in LDM-treated hepatoma BEL-7402 cells. Conelusion Mitotic cell death and senescence could be triggered simultaneously or sequentially after exposure of hepatoma BEL-7402 cells to LDM.The decrease in telomerase activity may play a key role in the defective mitosis and aging morphology.Further investigation of detailed mechanism is needed.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Effect of Lidamycin on Telomerase Activity in Human Hepatoma BEL-7402 Cells

Funds:  国家高技术研究发展计划(863计划)(2006AA02A255)%国家自然科学基金(30472042%30300424)

Abstract: Objective To investigate the effect of lidamycin(LDM)on telomerase activity in human hepatoma BEL-7402 cells under the condition of LDM inducing mitotic cell death and senescence.Methods Chromatin condensation was detected by co-staining with Hoechst 33342 and PI.Cell multinucleation was observed by Giemsa staining and genomic DNA was separated by agarose gel electrophoresis.Fluorescent intensity of Rho123 Was determined for mitochondrial membrane potential.MTT assay and SA-β-gal staining were employed to analyze the senescence-like phenotype.The expression of proteins was analyzed by Western blot.Telomerase activity was assayed by telomerase PCR-ELISA.Results Mitotic cell death occurred in LDM-treated cells characterized by unique and atypical chromatin condensation,multinucleation and increased mitochondrial membrane potential.However,no apoptotic bodies or DNA ladders were found.In addition,apoptosis-related proteins remained nearly unaltered.Senescence-like phenotype was identified by increased and elongated size of cells,growth retardation,enhanced SA-β-gal activity and the changes of senescence-related protein expression.Telomerase activity markedly decreased (P<0.01)in LDM-treated hepatoma BEL-7402 cells. Conelusion Mitotic cell death and senescence could be triggered simultaneously or sequentially after exposure of hepatoma BEL-7402 cells to LDM.The decrease in telomerase activity may play a key role in the defective mitosis and aging morphology.Further investigation of detailed mechanism is needed.

RUI-JUAN GAO, YUE-XIN LIANG, DIAN-DONG LI, HONG-YIN ZHANG, YONG-SU ZHEN. Effect of Lidamycin on Telomerase Activity in Human Hepatoma BEL-7402 Cells[J]. Biomedical and Environmental Sciences, 2007, 20(3): 189-197.
Citation: RUI-JUAN GAO, YUE-XIN LIANG, DIAN-DONG LI, HONG-YIN ZHANG, YONG-SU ZHEN. Effect of Lidamycin on Telomerase Activity in Human Hepatoma BEL-7402 Cells[J]. Biomedical and Environmental Sciences, 2007, 20(3): 189-197.

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