Volume 21 Issue 1
Feb.  2008
Turn off MathJax
Article Contents

HONG-GANG LIU. Warthin-starry Silver Method Showing Particulate Matter in Macrophage[J]. Biomedical and Environmental Sciences, 2008, 21(1): 85-89.
Citation: HONG-GANG LIU. Warthin-starry Silver Method Showing Particulate Matter in Macrophage[J]. Biomedical and Environmental Sciences, 2008, 21(1): 85-89.

Warthin-starry Silver Method Showing Particulate Matter in Macrophage

Funds:  The Electively Sponsored Project for Scientific Research of Returnees After Study Abroad
  • Objective To verify whether Warthin-Starry(WS)silver method could detect the air particulate matter(PM)/dust particles(Ps)located within the macrophages in situ. Methods There were 26 antopsy cases that resulted from cerebral hemorrhage(group A),silicosis(group B),and fetal death during pregnancy(group C).Samples were collected separately and serial sections were prepared from the lungs and lymph nodes and stained with hematoxylin and eosin(HE),WS silver,immunohistochemistry of CD68.Furthermore,ultrathin sections were taken from the WS positive serial sections of groups A and B.Ps were observed under a transmission electron microscope(TEM)and the elements of Ps were measured by X-ray spectrum analysis(X-RSA).Results In both groups A and B,WS staining was positive for the larger and fine Ps,the so called"dust cells",but HE staining Was almost negative for fine Ps.In group C,no larger or fine Ps were found.Immunohistochemical staining of CD68 certified that the"dust cells"containing Ps were macrophages.The results of TEM and X-RSA proved that the structure and elements of Ps belonged to PM indeed.Conclusion WS staining is a better than HE staining in showing the location of PM within macrophages.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1252) PDF downloads(15) Cited by()

Proportional views
Related

Warthin-starry Silver Method Showing Particulate Matter in Macrophage

Funds:  The Electively Sponsored Project for Scientific Research of Returnees After Study Abroad

Abstract: Objective To verify whether Warthin-Starry(WS)silver method could detect the air particulate matter(PM)/dust particles(Ps)located within the macrophages in situ. Methods There were 26 antopsy cases that resulted from cerebral hemorrhage(group A),silicosis(group B),and fetal death during pregnancy(group C).Samples were collected separately and serial sections were prepared from the lungs and lymph nodes and stained with hematoxylin and eosin(HE),WS silver,immunohistochemistry of CD68.Furthermore,ultrathin sections were taken from the WS positive serial sections of groups A and B.Ps were observed under a transmission electron microscope(TEM)and the elements of Ps were measured by X-ray spectrum analysis(X-RSA).Results In both groups A and B,WS staining was positive for the larger and fine Ps,the so called"dust cells",but HE staining Was almost negative for fine Ps.In group C,no larger or fine Ps were found.Immunohistochemical staining of CD68 certified that the"dust cells"containing Ps were macrophages.The results of TEM and X-RSA proved that the structure and elements of Ps belonged to PM indeed.Conclusion WS staining is a better than HE staining in showing the location of PM within macrophages.

HONG-GANG LIU. Warthin-starry Silver Method Showing Particulate Matter in Macrophage[J]. Biomedical and Environmental Sciences, 2008, 21(1): 85-89.
Citation: HONG-GANG LIU. Warthin-starry Silver Method Showing Particulate Matter in Macrophage[J]. Biomedical and Environmental Sciences, 2008, 21(1): 85-89.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return