Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes

SHUI-HUA ZHANG; JIA-XU LIANG; SHU-YAN DAI; XIAO-LIN QIU; YAN-RONG YI; YUN PAN

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Volume 22 Issue 5

Oct. 2009

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Article Navigation > Biomedical and Environmental Sciences > 2009 > 22(5): 388-393

SHUI-HUA ZHANG, JIA-XU LIANG, SHU-YAN DAI, XIAO-LIN QIU, YAN-RONG YI, YUN PAN. Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes[J]. Biomedical and Environmental Sciences, 2009, 22(5): 388-393.

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SHUI-HUA ZHANG, JIA-XU LIANG, SHU-YAN DAI, XIAO-LIN QIU, YAN-RONG YI, YUN PAN. Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes[J]. Biomedical and Environmental Sciences, 2009, 22(5): 388-393.

Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes

College of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai 200240, China;Shenzhen Neptunus Interlong Bio-technique Company, Ltd, Shenzhen 518054, Guangdong, China
Shenzhen Neptunus Interlong Bio-technique Company, Ltd, Shenzhen 518054, Guangdong, China

Abstract

Objective To elevate the immunological effect of subunit influenza vaccine in infants and aged people (over 60) using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation between vaccine antigens and liposomal characteristics. Methods Several formulations of liposomal subunit influenza vaccine were prepared. Their relevant characteristics were investigated to optimize the preparation method. Antisera obtained from immunizinged mice were used to evaluate the antibody titers of various samples by HI and ELISA. Results Liposomal trivalent influenza vaccine prepared by film evaporation in combinedation with freeze-drying significantly increased its immunological effect in SPF Balb/c mice. Liposomal vaccine stimulated the antibody titer of H3N2, H1N1, and B much stronger than conventional influenza vaccine. As a result, liposomal vaccine (mean size: 4.5-5.5 μm, entrapment efficiency: 30%-40%) significantly increased the immunological effect of subunit influenza vaccine. Conclusion The immune effect of liposomal vaccine depends on different antigens, and enhanced immunity is not positively correlated with the mean size of liposome or its entrapped efficiency.
- Liposome,
- Subunit influenza vaccine,
- Size distribution,
- Mean size,
- Entrapped efficiency
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes

College of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai 200240, China;Shenzhen Neptunus Interlong Bio-technique Company, Ltd, Shenzhen 518054, Guangdong, China

Shenzhen Neptunus Interlong Bio-technique Company, Ltd, Shenzhen 518054, Guangdong, China

Key words:

Abstract: Objective To elevate the immunological effect of subunit influenza vaccine in infants and aged people (over 60) using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation between vaccine antigens and liposomal characteristics. Methods Several formulations of liposomal subunit influenza vaccine were prepared. Their relevant characteristics were investigated to optimize the preparation method. Antisera obtained from immunizinged mice were used to evaluate the antibody titers of various samples by HI and ELISA. Results Liposomal trivalent influenza vaccine prepared by film evaporation in combinedation with freeze-drying significantly increased its immunological effect in SPF Balb/c mice. Liposomal vaccine stimulated the antibody titer of H3N2, H1N1, and B much stronger than conventional influenza vaccine. As a result, liposomal vaccine (mean size: 4.5-5.5 μm, entrapment efficiency: 30%-40%) significantly increased the immunological effect of subunit influenza vaccine. Conclusion The immune effect of liposomal vaccine depends on different antigens, and enhanced immunity is not positively correlated with the mean size of liposome or its entrapped efficiency.

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