Evaluation of Two Anti-Hepatitis E Virus IgM Kits

SHENG-XIANG GE; YING-JIE ZHENG; QING-SHUN GUO; JUN ZHANG; QING-WU JIANG; MUN-HON NG; NING-SHAO XIA

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Volume 20 Issue 6

Dec. 2007

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Article Navigation > Biomedical and Environmental Sciences > 2007 > 20(6): 512-515

SHENG-XIANG GE, YING-JIE ZHENG, QING-SHUN GUO, JUN ZHANG, QING-WU JIANG, MUN-HON NG, NING-SHAO XIA. Evaluation of Two Anti-Hepatitis E Virus IgM Kits[J]. Biomedical and Environmental Sciences, 2007, 20(6): 512-515.

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SHENG-XIANG GE, YING-JIE ZHENG, QING-SHUN GUO, JUN ZHANG, QING-WU JIANG, MUN-HON NG, NING-SHAO XIA. Evaluation of Two Anti-Hepatitis E Virus IgM Kits[J]. Biomedical and Environmental Sciences, 2007, 20(6): 512-515.

Evaluation of Two Anti-Hepatitis E Virus IgM Kits

National Institute of Diagnostics and Vaccine Development for Infectious Diseases, Xiamen University, Xiamen 361005, Fujian, China; Fujian Provincial Research Center for Medical Molecular Virology, Xiamen University, Xiamen 361005, Fujian, China
Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China

Funds: 国家高技术研究发展计划(863计划)%福建省自然科学基金(2002F013%2004YZ01)

Abstract

Objective To evaluate two commercial anti-hepatitis E virus (HEV) IgM kits used for differential diagnosis of acute enteric viral hepatitis. Methods The kit for IgM capture assay, was produced with a recombinant HEV structural protein protecting primates against experimental infection by different HEV genotypes, while the other kit for indirect ELISA was produced with recombinant structural proteins from different HEV genotypes. The serum specimens were taken from 241cases with a confirmed or presumptive diagnosis of hepatitis A and 74 cases with a confirmed or presumptive diagnosis of hepatitis E. Results The sensitivity and specificity of the IgM capture assay kit were 97% and 100%, respectively, and the corresponding values for the other kit were 70% and 78%, respectively. Conclusion The IgM capture assay kit has higher sensitivity and specificity in diagnosing acute enteric viral hepatitis E.
- IgM anti-HEV,
- Viral hepatitis
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Evaluation of Two Anti-Hepatitis E Virus IgM Kits

National Institute of Diagnostics and Vaccine Development for Infectious Diseases, Xiamen University, Xiamen 361005, Fujian, China; Fujian Provincial Research Center for Medical Molecular Virology, Xiamen University, Xiamen 361005, Fujian, China

Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China

Funds: 国家高技术研究发展计划(863计划)%福建省自然科学基金(2002F013%2004YZ01)

Key words:

IgM anti-HEV /
Viral hepatitis

Abstract: Objective To evaluate two commercial anti-hepatitis E virus (HEV) IgM kits used for differential diagnosis of acute enteric viral hepatitis. Methods The kit for IgM capture assay, was produced with a recombinant HEV structural protein protecting primates against experimental infection by different HEV genotypes, while the other kit for indirect ELISA was produced with recombinant structural proteins from different HEV genotypes. The serum specimens were taken from 241cases with a confirmed or presumptive diagnosis of hepatitis A and 74 cases with a confirmed or presumptive diagnosis of hepatitis E. Results The sensitivity and specificity of the IgM capture assay kit were 97% and 100%, respectively, and the corresponding values for the other kit were 70% and 78%, respectively. Conclusion The IgM capture assay kit has higher sensitivity and specificity in diagnosing acute enteric viral hepatitis E.

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