Volume 16 Issue 4
Dec.  2003
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YUE-XIN LIANG, Zhen Wang, DIAN-DONG LI, JIAN-MIN JIANG, RONG-GUANG SHAO. Effects of Aging and Advanced Glycation on Gene Expression in Cerebrum and Spleen of Mice[J]. Biomedical and Environmental Sciences, 2003, 16(4): 323-332.
Citation: YUE-XIN LIANG, Zhen Wang, DIAN-DONG LI, JIAN-MIN JIANG, RONG-GUANG SHAO. Effects of Aging and Advanced Glycation on Gene Expression in Cerebrum and Spleen of Mice[J]. Biomedical and Environmental Sciences, 2003, 16(4): 323-332.

Effects of Aging and Advanced Glycation on Gene Expression in Cerebrum and Spleen of Mice

Funds:  This study was supported(grants G2000057010)%国家重点基础研究发展计划(973计划)(30070827)%国家自然科学基金
  • Objective To analyze the effects of aging or advanced glycation on gene expression in the cerebrum and spleen of female C57BL/6J mice. Methods The gene expression profile was determined by using cDNA expression arrays containing 588 cDNA. Results Aging and advanced glycation resulted in differential gene expression patterns of cerebrum and spleen compared with young mice. Among the 80 genes detected in cerebrum, 43 exhibited a change in mRNA ratios with aging or treatment. Thirty-four changes (79%) were common in aged and D-galactose treated mice,whereas the cerebrum from aged and AGE-lysine treated mice showed common changes in expression of 38 genes(88%). Of the 86 genes detected in spleen, 29 (34%) displayed an age-related decrease in expression, whereas 3 (3%) displayed an increase in expression levels with aging. Eighteen genes from the detectable genes exhibited expression changes in both cerebrum and spleen of mice.Conclusions The gene expression profiles of D-galactose and AGE-lysine treated mice resemble those of aged mice. Use of cDNA hybridization arrays may provide a promising tool to explore the mechanism of aging at a molecular level.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Effects of Aging and Advanced Glycation on Gene Expression in Cerebrum and Spleen of Mice

Funds:  This study was supported(grants G2000057010)%国家重点基础研究发展计划(973计划)(30070827)%国家自然科学基金

Abstract: Objective To analyze the effects of aging or advanced glycation on gene expression in the cerebrum and spleen of female C57BL/6J mice. Methods The gene expression profile was determined by using cDNA expression arrays containing 588 cDNA. Results Aging and advanced glycation resulted in differential gene expression patterns of cerebrum and spleen compared with young mice. Among the 80 genes detected in cerebrum, 43 exhibited a change in mRNA ratios with aging or treatment. Thirty-four changes (79%) were common in aged and D-galactose treated mice,whereas the cerebrum from aged and AGE-lysine treated mice showed common changes in expression of 38 genes(88%). Of the 86 genes detected in spleen, 29 (34%) displayed an age-related decrease in expression, whereas 3 (3%) displayed an increase in expression levels with aging. Eighteen genes from the detectable genes exhibited expression changes in both cerebrum and spleen of mice.Conclusions The gene expression profiles of D-galactose and AGE-lysine treated mice resemble those of aged mice. Use of cDNA hybridization arrays may provide a promising tool to explore the mechanism of aging at a molecular level.

YUE-XIN LIANG, Zhen Wang, DIAN-DONG LI, JIAN-MIN JIANG, RONG-GUANG SHAO. Effects of Aging and Advanced Glycation on Gene Expression in Cerebrum and Spleen of Mice[J]. Biomedical and Environmental Sciences, 2003, 16(4): 323-332.
Citation: YUE-XIN LIANG, Zhen Wang, DIAN-DONG LI, JIAN-MIN JIANG, RONG-GUANG SHAO. Effects of Aging and Advanced Glycation on Gene Expression in Cerebrum and Spleen of Mice[J]. Biomedical and Environmental Sciences, 2003, 16(4): 323-332.

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