Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226

HAO ZHANG; NA LI; YUE CHEN; LING-YUN HUANG; YI-CHING WANG; GANG FANG; DA-CHENG HE; XUE-YUAN XIAO

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Volume 20 Issue 1

Feb. 2007

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Article Navigation > Biomedical and Environmental Sciences > 2007 > 20(1): 24-32

HAO ZHANG, NA LI, YUE CHEN, LING-YUN HUANG, YI-CHING WANG, GANG FANG, DA-CHENG HE, XUE-YUAN XIAO. Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226[J]. Biomedical and Environmental Sciences, 2007, 20(1): 24-32.

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HAO ZHANG, NA LI, YUE CHEN, LING-YUN HUANG, YI-CHING WANG, GANG FANG, DA-CHENG HE, XUE-YUAN XIAO. Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226[J]. Biomedical and Environmental Sciences, 2007, 20(1): 24-32.

Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226

Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Universities' Confederated Institute of Proteomics, Beijing Normal University, Beijing 100875, China
Department of Life Sciences, National Taiwan Normal Universities, Sec. 4 Tingchou Rd, Taipei 116, Taiwan

Funds: 国家自然科学基金(30370712)%Beijing Key Project(7051002)

Abstract

Objective To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. Method Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Results The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95±0.53 mm in the isoelectric focusing direction,and 1.73±0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis.Conclusion The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer.
- Lung squamous carcinoma,
- NCI-H226 cell line,
- Proteomics,
- Two-dimensional datapase
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226

Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Universities' Confederated Institute of Proteomics, Beijing Normal University, Beijing 100875, China

Department of Life Sciences, National Taiwan Normal Universities, Sec. 4 Tingchou Rd, Taipei 116, Taiwan

Funds: 国家自然科学基金(30370712)%Beijing Key Project(7051002)

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Abstract: Objective To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. Method Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Results The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95±0.53 mm in the isoelectric focusing direction,and 1.73±0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis.Conclusion The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer.

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