Volume 22 Issue 1
Feb.  2009
Turn off MathJax
Article Contents

QIN ZHUO, JIAN-HUA PIAO, YUAN TIAN, JIE XU, XIAO-GUANG YANG. Large-scale Purification and Acute Toxicity of Hygromycin B Phosphotransferase[J]. Biomedical and Environmental Sciences, 2009, 22(1): 22-27.
Citation: QIN ZHUO, JIAN-HUA PIAO, YUAN TIAN, JIE XU, XIAO-GUANG YANG. Large-scale Purification and Acute Toxicity of Hygromycin B Phosphotransferase[J]. Biomedical and Environmental Sciences, 2009, 22(1): 22-27.

Large-scale Purification and Acute Toxicity of Hygromycin B Phosphotransferase

Funds:  国家重点基础研究发展规划(973计划)(2007CB 109207)
  • Objective To provide the acute toxicity data of hygromycin B phosphotransferase (HPT) using recombinant protein purified from E. coli. Methods Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared as recombinant HPT protein. One hundred mice, randomly assigned to 5 groups, were administrated 10 g/kg, 5 g/kg, or 1 g/kg body weight of HPT or 5 g/kg body weight of bacterial control protein or phosphate-buffered saline (PBS) respectively by oral gavage. Results All animals survived with no significant change in body weight gain throughout the study. Macroscopic necropsy examination on day 15 revealed no gross pathological lesions in any of the animals. The maximum tolerated dose (MTD) of HPT was 10 g/kg body weight in mice and could be regarded as nontoxic. Conclusion HPT protein does not have any safety problems to human health.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1038) PDF downloads(17) Cited by()

Proportional views
Related

Large-scale Purification and Acute Toxicity of Hygromycin B Phosphotransferase

Funds:  国家重点基础研究发展规划(973计划)(2007CB 109207)

Abstract: Objective To provide the acute toxicity data of hygromycin B phosphotransferase (HPT) using recombinant protein purified from E. coli. Methods Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared as recombinant HPT protein. One hundred mice, randomly assigned to 5 groups, were administrated 10 g/kg, 5 g/kg, or 1 g/kg body weight of HPT or 5 g/kg body weight of bacterial control protein or phosphate-buffered saline (PBS) respectively by oral gavage. Results All animals survived with no significant change in body weight gain throughout the study. Macroscopic necropsy examination on day 15 revealed no gross pathological lesions in any of the animals. The maximum tolerated dose (MTD) of HPT was 10 g/kg body weight in mice and could be regarded as nontoxic. Conclusion HPT protein does not have any safety problems to human health.

QIN ZHUO, JIAN-HUA PIAO, YUAN TIAN, JIE XU, XIAO-GUANG YANG. Large-scale Purification and Acute Toxicity of Hygromycin B Phosphotransferase[J]. Biomedical and Environmental Sciences, 2009, 22(1): 22-27.
Citation: QIN ZHUO, JIAN-HUA PIAO, YUAN TIAN, JIE XU, XIAO-GUANG YANG. Large-scale Purification and Acute Toxicity of Hygromycin B Phosphotransferase[J]. Biomedical and Environmental Sciences, 2009, 22(1): 22-27.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return