Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells

LONG-GUO YUAN; HONG-BIN DENG; LI-HUI CHEN; DIAN-DONG LI; QI-YANG HE

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Volume 21 Issue 3

Jun. 2008

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Article Navigation > Biomedical and Environmental Sciences > 2008 > 21(3): 212-217

LONG-GUO YUAN, HONG-BIN DENG, LI-HUI CHEN, DIAN-DONG LI, QI-YANG HE. Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells[J]. Biomedical and Environmental Sciences, 2008, 21(3): 212-217.

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LONG-GUO YUAN, HONG-BIN DENG, LI-HUI CHEN, DIAN-DONG LI, QI-YANG HE. Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells[J]. Biomedical and Environmental Sciences, 2008, 21(3): 212-217.

Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells

Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China

Funds: 国家重点基础研究发展计划(973计划)(2007CB507406)%国家自然科学基金(30600659)

Abstract

Objective To study whether Lycium barbarian glycopeptide 3 (LBGP3) affects T cell apeptosis in aged mice. Methods LBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic "sub-Gl peak" was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-γ, and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FLIP, FasL, and Bcl-2 were determined by Western blotting. Resdts LBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide.Treatment with 200 μg/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T ceils. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FLIP, and up-regulating the expression of FasL. Conclusion Lycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules.
- Lycium barbarum glycopeptide 3,
- Aged T cells,
- Cytokines,
- Apoptosis,
- Senescence
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells

Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, China

Funds: 国家重点基础研究发展计划(973计划)(2007CB507406)%国家自然科学基金(30600659)

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Abstract: Objective To study whether Lycium barbarian glycopeptide 3 (LBGP3) affects T cell apeptosis in aged mice. Methods LBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic "sub-Gl peak" was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-γ, and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FLIP, FasL, and Bcl-2 were determined by Western blotting. Resdts LBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide.Treatment with 200 μg/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T ceils. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FLIP, and up-regulating the expression of FasL. Conclusion Lycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules.

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