Gene Expression Profile of Human Skeletal Muscle and Adipose Tissue of Chinese Han Patients with Type 2 Diabetes Mellitus

YAN-LI YANG; RUO-LAN XIANG; CHANG YANG; XIAO-JUN LIU; WEN-JUN SHEN; JIN ZUO; YONG-SHENG CHANG; FU-DE FANG

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Volume 22 Issue 5

Oct. 2009

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Article Navigation > Biomedical and Environmental Sciences > 2009 > 22(5): 359-368

YAN-LI YANG, RUO-LAN XIANG, CHANG YANG, XIAO-JUN LIU, WEN-JUN SHEN, JIN ZUO, YONG-SHENG CHANG, FU-DE FANG. Gene Expression Profile of Human Skeletal Muscle and Adipose Tissue of Chinese Han Patients with Type 2 Diabetes Mellitus[J]. Biomedical and Environmental Sciences, 2009, 22(5): 359-368.

Citation:

YAN-LI YANG, RUO-LAN XIANG, CHANG YANG, XIAO-JUN LIU, WEN-JUN SHEN, JIN ZUO, YONG-SHENG CHANG, FU-DE FANG. Gene Expression Profile of Human Skeletal Muscle and Adipose Tissue of Chinese Han Patients with Type 2 Diabetes Mellitus[J]. Biomedical and Environmental Sciences, 2009, 22(5): 359-368.

Gene Expression Profile of Human Skeletal Muscle and Adipose Tissue of Chinese Han Patients with Type 2 Diabetes Mellitus

Department of Internal Medicine, Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China
Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing 100083, China
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100005, China
Division of Endocrinology, Stanford University and VA Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, CA 94304, USA

Funds: the National High Technology Research and Development Program of China (863 Program)(2001AA221161)%the National Key Technologies R & D Program of China(2002BA711A05)

Abstract

Objective To study the differential patterns of gene expression in skeletal muscle and adipose tissue between type 2 diabetes mellitus (T2DM) patients and healthy subjects using DNA microarray analysis. Methods T2DM patiens were divided into female group, young male group and old male group. DNA microarray analysis and quantitative real-time PCR were carried out to analyze the relation between gene expressions and T2DM. Results The mRNA expression of 298, 578, and 350 genes was changed in the skeletal muscle of diabetes mellitus patients compared with control subjects. The 1320, 1143, and 2847 genes were modified in adipose tissue of the three groups. Among the genes surveyed, the change of 25 and 39 gene transcripts in skeletal muscle and adipose tissue was ≥2 folds. These differentially expressed genes were classified into 15 categories according to their functions. Conclusion New genes are found and T2DM can be prevented or cured.
- Type 2 diabetes mellitus (T2DM),
- Microarray technology,
- Skeletal muscle,
- Adipose tissue
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Gene Expression Profile of Human Skeletal Muscle and Adipose Tissue of Chinese Han Patients with Type 2 Diabetes Mellitus

Department of Internal Medicine, Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China

Department of Physiology and Pathophysiology, Peking University Health Science Center, Beijing 100083, China

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100005, China

Division of Endocrinology, Stanford University and VA Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, CA 94304, USA

Funds: the National High Technology Research and Development Program of China (863 Program)(2001AA221161)%the National Key Technologies R & D Program of China(2002BA711A05)

Key words:

Abstract: Objective To study the differential patterns of gene expression in skeletal muscle and adipose tissue between type 2 diabetes mellitus (T2DM) patients and healthy subjects using DNA microarray analysis. Methods T2DM patiens were divided into female group, young male group and old male group. DNA microarray analysis and quantitative real-time PCR were carried out to analyze the relation between gene expressions and T2DM. Results The mRNA expression of 298, 578, and 350 genes was changed in the skeletal muscle of diabetes mellitus patients compared with control subjects. The 1320, 1143, and 2847 genes were modified in adipose tissue of the three groups. Among the genes surveyed, the change of 25 and 39 gene transcripts in skeletal muscle and adipose tissue was ≥2 folds. These differentially expressed genes were classified into 15 categories according to their functions. Conclusion New genes are found and T2DM can be prevented or cured.

Citation:

YAN-LI YANG, RUO-LAN XIANG, CHANG YANG, XIAO-JUN LIU, WEN-JUN SHEN, JIN ZUO, YONG-SHENG CHANG, FU-DE FANG. Gene Expression Profile of Human Skeletal Muscle and Adipose Tissue of Chinese Han Patients with Type 2 Diabetes Mellitus[J]. Biomedical and Environmental Sciences, 2009, 22(5): 359-368.