Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells

HUA-JIE CHEN; RI-AN YU; LING-FEI HE; SHE-JUAN AN; ZHI-GANG WU; KE-DI YANG; XUE-MIN CHEN

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Volume 20 Issue 4

Aug. 2007

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Article Navigation > Biomedical and Environmental Sciences > 2007 > 20(4): 307-312

HUA-JIE CHEN, RI-AN YU, LING-FEI HE, SHE-JUAN AN, ZHI-GANG WU, KE-DI YANG, XUE-MIN CHEN. Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells[J]. Biomedical and Environmental Sciences, 2007, 20(4): 307-312.

Citation:

HUA-JIE CHEN, RI-AN YU, LING-FEI HE, SHE-JUAN AN, ZHI-GANG WU, KE-DI YANG, XUE-MIN CHEN. Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells[J]. Biomedical and Environmental Sciences, 2007, 20(4): 307-312.

Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells

Department of Occupational and Environmental Health, MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
Department of Occupational and Environmental Health, School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong, China
Department of Dental Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, Guangdong, China

Funds: Scientific Foundation of Guangdong Pharmaceutical University(2006GGW01)%国家自然科学基金(30271110)

Abstract

Objective To investigate the effects of sodium selenite on telomerase activity and expression of hTERT mRNA in cadmium-transformed 16HBE cells. Methods Telomerase activity and expression of genes were measured after cultured cadmium-transformed 16HBE cells were exposed to sodium selenite at different doses (0.625, 1.25, 2.50, 5.00 μmol/L) for 24 hours. Results Selenium decreased telomerase activity in cadmium-transformed 16HBE cells. There existed an obvious dose-effect relationship between the selenium concentration and these changes. The expression of hTERT and c-myc mRNA also decreased but the expression of mad1 mRNA increased after exposure to selenium for 24 hours. No difference was found in expression of hTRF1 and hTRF2 mRNA after incubated with sodium selenite for 24 hours, compared with control group.Conclusion Selenium inhibits telomerase activity by decreasing hTERT and c-myc mRNA expression and increasing mad1 mRNA expression in cadmium-transformed 16HBE cells and selenium concentration is significantly correlated with these changes.
- Selenium,
- Cadmium,
- Telomerase,
- Human telomerase reverse transcriptase
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells

Department of Occupational and Environmental Health, MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China

Department of Occupational and Environmental Health, School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong, China

Department of Dental Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, Guangdong, China

Funds: Scientific Foundation of Guangdong Pharmaceutical University(2006GGW01)%国家自然科学基金(30271110)

Key words:

Abstract: Objective To investigate the effects of sodium selenite on telomerase activity and expression of hTERT mRNA in cadmium-transformed 16HBE cells. Methods Telomerase activity and expression of genes were measured after cultured cadmium-transformed 16HBE cells were exposed to sodium selenite at different doses (0.625, 1.25, 2.50, 5.00 μmol/L) for 24 hours. Results Selenium decreased telomerase activity in cadmium-transformed 16HBE cells. There existed an obvious dose-effect relationship between the selenium concentration and these changes. The expression of hTERT and c-myc mRNA also decreased but the expression of mad1 mRNA increased after exposure to selenium for 24 hours. No difference was found in expression of hTRF1 and hTRF2 mRNA after incubated with sodium selenite for 24 hours, compared with control group.Conclusion Selenium inhibits telomerase activity by decreasing hTERT and c-myc mRNA expression and increasing mad1 mRNA expression in cadmium-transformed 16HBE cells and selenium concentration is significantly correlated with these changes.

Citation:

HUA-JIE CHEN, RI-AN YU, LING-FEI HE, SHE-JUAN AN, ZHI-GANG WU, KE-DI YANG, XUE-MIN CHEN. Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells[J]. Biomedical and Environmental Sciences, 2007, 20(4): 307-312.