Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo

YAN-JUN GUO; JUN HAN; HAI-LAN YAO; BAO-YUN ZHANG; JIAN-MEI GAO; JIN ZHANG; XIN-LI XIAO; XIAO-FAN WANG; WEI-QIN ZHAO; DE-XIN WANG; XIAO-PING DONG

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Volume 20 Issue 3

Jun. 2007

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Article Navigation > Biomedical and Environmental Sciences > 2007 > 20(3): 198-202

YAN-JUN GUO, JUN HAN, HAI-LAN YAO, BAO-YUN ZHANG, JIAN-MEI GAO, JIN ZHANG, XIN-LI XIAO, XIAO-FAN WANG, WEI-QIN ZHAO, DE-XIN WANG, XIAO-PING DONG. Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo[J]. Biomedical and Environmental Sciences, 2007, 20(3): 198-202.

Citation:

YAN-JUN GUO, JUN HAN, HAI-LAN YAO, BAO-YUN ZHANG, JIAN-MEI GAO, JIN ZHANG, XIN-LI XIAO, XIAO-FAN WANG, WEI-QIN ZHAO, DE-XIN WANG, XIAO-PING DONG. Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo[J]. Biomedical and Environmental Sciences, 2007, 20(3): 198-202.

Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo

State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China;Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beifing 100050,China
State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China
Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China

Funds: 国家自然科学基金(30130070)%国家高技术研究发展计划(863计划)(2001AA215391)%National Science and Technology Task Force Project(2001AA215391)%EU Project QLRT(2000.01441)

Abstract

Objective To study the possible effect of tetracycline on protease-resistant activity in vitro and infectivity in vivo of a scrapie strain 263K.Methods Scrapie pathogens were incubated with tetracycline at different concentrations for various periods of time and protease-resistant PrP signals were evaluated with proteinase K-treatment and Western blots.The preparations treated with tetracycline were intracerebrally inoculated into golden hamsters and typical TSE manifestations were noted.PrPSc in brain tissues of the infected animals was detected by PrP specific Western blot assays. Results Protease-resistant PrP was significantly reduced in or removed from the preparations treated with tetracycline in a dose-dependant manner.Compared with the control group after incubated for 53.75±0.50 days,the preparations treated with 5 mmol/L and 20 mmol/L tetracycline prolonged the incubation time of 61.5±1.73 and 59.5±0.58 days (P＜0.05). Conclusion Treatment of scrapie pathogen 263K with tetracycline reduces or removes its protease-resistant activity in vitro.
- Scrapie,
- Prion,
- Tetracycline,
- Protease-resistant activity,
- Animal experiment
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo

State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China;Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beifing 100050,China

State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China

Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China

Funds: 国家自然科学基金(30130070)%国家高技术研究发展计划(863计划)(2001AA215391)%National Science and Technology Task Force Project(2001AA215391)%EU Project QLRT(2000.01441)

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Abstract: Objective To study the possible effect of tetracycline on protease-resistant activity in vitro and infectivity in vivo of a scrapie strain 263K.Methods Scrapie pathogens were incubated with tetracycline at different concentrations for various periods of time and protease-resistant PrP signals were evaluated with proteinase K-treatment and Western blots.The preparations treated with tetracycline were intracerebrally inoculated into golden hamsters and typical TSE manifestations were noted.PrPSc in brain tissues of the infected animals was detected by PrP specific Western blot assays. Results Protease-resistant PrP was significantly reduced in or removed from the preparations treated with tetracycline in a dose-dependant manner.Compared with the control group after incubated for 53.75±0.50 days,the preparations treated with 5 mmol/L and 20 mmol/L tetracycline prolonged the incubation time of 61.5±1.73 and 59.5±0.58 days (P＜0.05). Conclusion Treatment of scrapie pathogen 263K with tetracycline reduces or removes its protease-resistant activity in vitro.

Citation:

YAN-JUN GUO, JUN HAN, HAI-LAN YAO, BAO-YUN ZHANG, JIAN-MEI GAO, JIN ZHANG, XIN-LI XIAO, XIAO-FAN WANG, WEI-QIN ZHAO, DE-XIN WANG, XIAO-PING DONG. Treatment of Scrapie Pathogen 263K With Tetracycline Partially Abolishes Protease-resistant Activity in vitro and Reduces Infectivity in vivo[J]. Biomedical and Environmental Sciences, 2007, 20(3): 198-202.