Inhibitory Effects of Saponins From Anemarrhena asphodeloides Bunge on the Growth of Vascular Smooth Muscle Cells

SHANG-ZHI XIAO; MING-EN XU; YA-KUN GE; GUI-FENG XIAO

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Volume 19 Issue 3

Jun. 2006

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Article Navigation > Biomedical and Environmental Sciences > 2006 > 19(3): 185-191

SHANG-ZHI XIAO, MING-EN XU, YA-KUN GE, GUI-FENG XIAO. Inhibitory Effects of Saponins From Anemarrhena asphodeloides Bunge on the Growth of Vascular Smooth Muscle Cells[J]. Biomedical and Environmental Sciences, 2006, 19(3): 185-191.

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SHANG-ZHI XIAO, MING-EN XU, YA-KUN GE, GUI-FENG XIAO. Inhibitory Effects of Saponins From Anemarrhena asphodeloides Bunge on the Growth of Vascular Smooth Muscle Cells[J]. Biomedical and Environmental Sciences, 2006, 19(3): 185-191.

Inhibitory Effects of Saponins From Anemarrhena asphodeloides Bunge on the Growth of Vascular Smooth Muscle Cells

Department of Biomedical Engineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, Zhejiang, China

Abstract

Objective To investigate the effects of saponins from Anemarrhena asphodeloides Bunge (SAaB) (Botanical Name: Anemarrhena Asphodeloidis Rhizoma) on the growth of vascular smooth muscle cells (VSMCs). Methods Cell proliferation was measured by a newly developed cell proliferation reagent, WST-1. Cell apoptosis was assayed by flow cytometry through detecting annexin V. Nitric oxide production was evaluated using confocal laser scanning microscopy with diaminofluorescein diacetate (DAF-2, DA). Cell aldose reductase (AR) activity, as well as the effect of Epalrestat and interleukin-1β were also explored. Results WST assay showed that cell proliferation induced by serum was significantly inhibited by SAaB (P＜0.01). Flow cytometry analysis revealed that SAaB could enhance apoptotic rate of VSMCs (P＜0.01). Nitric oxide production was significantly enhanced after administration of SAaB and interleukin- 1β. Moreover, AR activity of VSMCs was also remarkably inhibited by both SAaB and Epalrestat (P＜0.01). Conclusion SAaB can inhibit proliferation and enhance apoptosis of VSMCs. It may protect vascular cells by inhibiting VSMC proliferation and augmenting apoptotic rate of VSMCs via NO-dependent pathway.
- Anemarrhena asphodeloides Bunge,
- Saponins,
- Vascular smooth muscle cells,
- Proliferation
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Inhibitory Effects of Saponins From Anemarrhena asphodeloides Bunge on the Growth of Vascular Smooth Muscle Cells

Department of Biomedical Engineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, Zhejiang, China

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Abstract: Objective To investigate the effects of saponins from Anemarrhena asphodeloides Bunge (SAaB) (Botanical Name: Anemarrhena Asphodeloidis Rhizoma) on the growth of vascular smooth muscle cells (VSMCs). Methods Cell proliferation was measured by a newly developed cell proliferation reagent, WST-1. Cell apoptosis was assayed by flow cytometry through detecting annexin V. Nitric oxide production was evaluated using confocal laser scanning microscopy with diaminofluorescein diacetate (DAF-2, DA). Cell aldose reductase (AR) activity, as well as the effect of Epalrestat and interleukin-1β were also explored. Results WST assay showed that cell proliferation induced by serum was significantly inhibited by SAaB (P＜0.01). Flow cytometry analysis revealed that SAaB could enhance apoptotic rate of VSMCs (P＜0.01). Nitric oxide production was significantly enhanced after administration of SAaB and interleukin- 1β. Moreover, AR activity of VSMCs was also remarkably inhibited by both SAaB and Epalrestat (P＜0.01). Conclusion SAaB can inhibit proliferation and enhance apoptosis of VSMCs. It may protect vascular cells by inhibiting VSMC proliferation and augmenting apoptotic rate of VSMCs via NO-dependent pathway.

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