Damage to Hippocampus of Rats after Being Exposed to Infrasound

ZHANG Meng Yao; CHEN Chen; XIE Xue Jun; XU Sheng Long; GUO Guo Zhen; WANG Jin

doi:10.3967/bes2016.056

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Volume 29 Issue 6

Jun. 2016

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Article Navigation > Biomedical and Environmental Sciences > 2016 > 29(6): 435-442

ZHANG Meng Yao, CHEN Chen, XIE Xue Jun, XU Sheng Long, GUO Guo Zhen, WANG Jin. Damage to Hippocampus of Rats after Being Exposed to Infrasound[J]. Biomedical and Environmental Sciences, 2016, 29(6): 435-442. doi: 10.3967/bes2016.056

Citation:

ZHANG Meng Yao, CHEN Chen, XIE Xue Jun, XU Sheng Long, GUO Guo Zhen, WANG Jin. Damage to Hippocampus of Rats after Being Exposed to Infrasound[J]. Biomedical and Environmental Sciences, 2016, 29(6): 435-442. doi: 10.3967/bes2016.056

Damage to Hippocampus of Rats after Being Exposed to Infrasound

doi: 10.3967/bes2016.056

Beijing Institute of Biotechnology, Fengtai District, Beijing 100071, China
Department of Blood Transfusion, Xijing Hospital of Fourth Military Medical University, Xi'an 710032, Shaanxi, China
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi, China

Abstract

Objective The objective was to observe damage of hippocampus in rats after exposure to infrasound, and to assess HSP70 expression in hippocampus.
Methods SD rats in the experimental group were exposed to 140 dB (8 Hz) infrasound for 2 h per day for 3 days. The morphology of the hippocampus was examined by transmission electronic microscopic (TEM). Cell apoptosis was observed by TUNEL staining at 0 h, 24 h, 48 h, and 2 w after exposure. HSP70 expression was detected by immunohistochemistry (IHC) and Western blotting (WB).
Results TEM showed that hippocampus was significantly damaged by exposure, and exhibited recovery 1 week after exposure. The TUNEL data showed that neuronal apoptosis after exposure was significantly higher than in the control rats at 24 h and 48 h, and the apoptotic cells decreased one week after exposure. IHC and WB showed HSP70 expression was significantly higher in the exposed rats, peaked at 24 h.
Conclusion Exposure to 140 dB (8 Hz) infrasound for 2 h per day for 3 days appeared to induce damage to the hippocampus of rats, based on changes in ultrastructure and increased cell apoptosis. However, recovery from the damage occurred overtime. HSP70 expression also increased after the exposure and decreased by 48 h.
- Infrasound,
- Hippocampus,
- Apoptosis,
- HSP70
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Damage to Hippocampus of Rats after Being Exposed to Infrasound

doi: 10.3967/bes2016.056

Beijing Institute of Biotechnology, Fengtai District, Beijing 100071, China

Department of Blood Transfusion, Xijing Hospital of Fourth Military Medical University, Xi'an 710032, Shaanxi, China

Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi, China

Key words:

Infrasound /
Hippocampus /
Apoptosis /
HSP70

Abstract: Objective The objective was to observe damage of hippocampus in rats after exposure to infrasound, and to assess HSP70 expression in hippocampus.
Methods SD rats in the experimental group were exposed to 140 dB (8 Hz) infrasound for 2 h per day for 3 days. The morphology of the hippocampus was examined by transmission electronic microscopic (TEM). Cell apoptosis was observed by TUNEL staining at 0 h, 24 h, 48 h, and 2 w after exposure. HSP70 expression was detected by immunohistochemistry (IHC) and Western blotting (WB).
Results TEM showed that hippocampus was significantly damaged by exposure, and exhibited recovery 1 week after exposure. The TUNEL data showed that neuronal apoptosis after exposure was significantly higher than in the control rats at 24 h and 48 h, and the apoptotic cells decreased one week after exposure. IHC and WB showed HSP70 expression was significantly higher in the exposed rats, peaked at 24 h.
Conclusion Exposure to 140 dB (8 Hz) infrasound for 2 h per day for 3 days appeared to induce damage to the hippocampus of rats, based on changes in ultrastructure and increased cell apoptosis. However, recovery from the damage occurred overtime. HSP70 expression also increased after the exposure and decreased by 48 h.

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