Development of An Enzyme-Linked Immunosorbent Assay for Determination of the Furaltadone Etabolite, 3-Amino-5-Morpholinomethyl-2-Oxazolidinone (AMOZ) in Animal Tissues
doi: 10.3967/0895-3988.2012.04.010
-
Key words:
- AMOZ /
- Animal tissue /
- ELISA /
- Fish tissue /
- Furaltadone
Abstract: Objective To determine 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) residues released from protein bound AMOZ in animal tissues.Methods Polyclonal and monoclonal antibodies were produced in this study.A rapid,sensitive,and specific competitive direct enzyme-linked immunosorbent assay (cdELISA) was developed.Results Rabbit polyclonal antibodies were used in the optimized cdELISA method,and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ.The ICso of the polyclonal antibody was 0.16 ng/mL.The method limit of detection in four different types of animal and fish tissues was less than 0.06 ug/kg.Recoveries ranged from 80% to 120% for fortified samples with the coefficient of variation values less than 15%.The results of the cdELISA method were in good agreement with the results from an established liquid chromatography-tandem mass spectrometry confirmatory method used for AMOZ residues.Conclusion The cdELISA method developed in the present study is a convenient practical tool for screening large numbers of animal and fish tissue samples for the the detection of released protein bound AMOZ residues.
Citation: | LUO Peng Jie, JIANG Wen Xiao, BEIER Ross C., SHEN Jian Zhong, JIANG Hai Yang, MIAO Hong, ZHAO Yun Feng, CHEN Xia, WU Yong Ning. Development of An Enzyme-Linked Immunosorbent Assay for Determination of the Furaltadone Etabolite, 3-Amino-5-Morpholinomethyl-2-Oxazolidinone (AMOZ) in Animal Tissues[J]. Biomedical and Environmental Sciences, 2012, 25(4): 449-457. doi: 10.3967/0895-3988.2012.04.010 |