WANG YanHua; HAI Rong; ZHANG ZhiKai; XIA LianXu; CAI Hong; LIANG Ying; SHEN XiaoNa; YU DongZheng

doi:10.3967/0895-3988.2011.03.015

Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries

WANG YanHua, HAI Rong, ZHANG ZhiKai, XIA LianXu, CAI Hong, LIANG Ying, SHEN XiaoNa, YU DongZheng

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(3): 310-314

WANG YanHua, HAI Rong, ZHANG ZhiKai, XIA LianXu, CAI Hong, LIANG Ying, SHEN XiaoNa, YU DongZheng. Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries[J]. Biomedical and Environmental Sciences, 2011, 24(3): 310-314. doi: 10.3967/0895-3988.2011.03.015

Citation:

WANG YanHua, HAI Rong, ZHANG ZhiKai, XIA LianXu, CAI Hong, LIANG Ying, SHEN XiaoNa, YU DongZheng. Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries[J]. Biomedical and Environmental Sciences, 2011, 24(3): 310-314. doi: 10.3967/0895-3988.2011.03.015

doi: 10.3967/0895-3988.2011.03.015

基金项目: the National Technology Key Special Study Program of the Eleventh Five-year Plan(2008ZX10004-010)

Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: the National Technology Key Special Study Program of the Eleventh Five-year Plan(2008ZX10004-010)

摘要: Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries.Methods Ten strains of F. tularensis isolated from China were amplified by using typing primers C1/C4 and RD1. On the basis of the lengths of the polymerase chain reaction (PCR) products, it was concluded that these strains of F. tularensis belonged to the same subspecies. At the same time, the fopA, tul4, and 16S rRNA genes of the 10 strains were amplified, and a three-gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0.Results The 10 strains of F. tularensis from China were all identified as belonging to subspecies holarctica (type B). We found no direct relationship between the genotypes of F. tularensis subsp.holarctica and the geographical area from where they were isolated.Conclusion The F. tularensis strains isolated from North China mainly belong to subspecies holarctica (type B). The strains of F. tularensis subsp. holarctica from China may have evolved earlier than those from Europe and North America.
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Abstract: Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries.Methods Ten strains of F. tularensis isolated from China were amplified by using typing primers C1/C4 and RD1. On the basis of the lengths of the polymerase chain reaction (PCR) products, it was concluded that these strains of F. tularensis belonged to the same subspecies. At the same time, the fopA, tul4, and 16S rRNA genes of the 10 strains were amplified, and a three-gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0.Results The 10 strains of F. tularensis from China were all identified as belonging to subspecies holarctica (type B). We found no direct relationship between the genotypes of F. tularensis subsp.holarctica and the geographical area from where they were isolated.Conclusion The F. tularensis strains isolated from North China mainly belong to subspecies holarctica (type B). The strains of F. tularensis subsp. holarctica from China may have evolved earlier than those from Europe and North America.
- Francisella tularensis /
- Tularemia /
- Phylogenetic analysis /
- Genotyping,PCR

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Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries

doi: 10.3967/0895-3988.2011.03.015

基金项目: the National Technology Key Special Study Program of the Eleventh Five-year Plan(2008ZX10004-010)

刊出日期: 2011-06-20

关键词:

Francisella tularensis /

Tularemia /

Phylogenetic analysis /

Genotyping,PCR

摘要: Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries.Methods Ten strains of F. tularensis isolated from China were amplified by using typing primers C1/C4 and RD1. On the basis of the lengths of the polymerase chain reaction (PCR) products, it was concluded that these strains of F. tularensis belonged to the same subspecies. At the same time, the fopA, tul4, and 16S rRNA genes of the 10 strains were amplified, and a three-gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0.Results The 10 strains of F. tularensis from China were all identified as belonging to subspecies holarctica (type B). We found no direct relationship between the genotypes of F. tularensis subsp.holarctica and the geographical area from where they were isolated.Conclusion The F. tularensis strains isolated from North China mainly belong to subspecies holarctica (type B). The strains of F. tularensis subsp. holarctica from China may have evolved earlier than those from Europe and North America.

English Abstract

Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: the National Technology Key Special Study Program of the Eleventh Five-year Plan(2008ZX10004-010)

Publish Date: 2011-06-20

Keywords:

Abstract: Objective To study the types of subspecies of Francisella tularensis from China and to investigate the genetic relationships between F. tularensis strains from China and from other countries.Methods Ten strains of F. tularensis isolated from China were amplified by using typing primers C1/C4 and RD1. On the basis of the lengths of the polymerase chain reaction (PCR) products, it was concluded that these strains of F. tularensis belonged to the same subspecies. At the same time, the fopA, tul4, and 16S rRNA genes of the 10 strains were amplified, and a three-gene based phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis software version 4.0.Results The 10 strains of F. tularensis from China were all identified as belonging to subspecies holarctica (type B). We found no direct relationship between the genotypes of F. tularensis subsp.holarctica and the geographical area from where they were isolated.Conclusion The F. tularensis strains isolated from North China mainly belong to subspecies holarctica (type B). The strains of F. tularensis subsp. holarctica from China may have evolved earlier than those from Europe and North America.

Citation:

WANG YanHua, HAI Rong, ZHANG ZhiKai, XIA LianXu, CAI Hong, LIANG Ying, SHEN XiaoNa, YU DongZheng. Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries[J]. Biomedical and Environmental Sciences, 2011, 24(3): 310-314. doi: 10.3967/0895-3988.2011.03.015

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doi: 10.3967/0895-3988.2011.03.015

Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries

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Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries

doi: 10.3967/0895-3988.2011.03.015

English Abstract

Genetic Relationship between Francisella Tularensis Strains from China and from Other Countries

State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

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