ZHAO Lei; LIU QingJun; CHEN Hong; DUAN HuaWei; BIN Ping; LIU Qing; NIU Yong; DAI YuFei; ZHENG YuXin

doi:10.3967/0895-3988.2011.04.008

doi: 10.3967/0895-3988.2011.04.008

基金项目: the National Nature Science Foundation of China(30700674%30625031)%the Project for Technologies of Occupational Health Surveillance and Detection(200902006)%the Youth Fund of Chinese Center of Disease Control(2010A204)

The Effect of 2,5-hexanedione on Myelin Protein Zero Expression, and Its Mitigation Using Ginkgo Biloba Extract

National Institute for Occupational Health and Poison Control and Key laboratory of Chemical Safety and Health,Chinese Center for Disease Control and Prevention, Beijing 100050, China

Funds: the National Nature Science Foundation of China(30700674%30625031)%the Project for Technologies of Occupational Health Surveillance and Detection(200902006)%the Youth Fund of Chinese Center of Disease Control(2010A204)

摘要: Objective To investigate the role of myelin protein zero (P0) in 2,5-hexanedione (2,5-HD)-induced peripheral nerve injury,and the protective effect of Ginkgo biloba extract (Egb761) on 2,5-HD-induced toxic peripheral neuropathy.Methods After 4 weeks of treatment with 2,5-HD at different doses (50,100,200,400 mg/kg) in rats,changes in the levels of P0 in rat sciatic nerves was investigated,and the effect of Egb761 on 2,5-HD-induced toxic peripheral neuropathy was studied.Results The blood-nerve barrier (BNB) permeability of the sciatic nerve increased,and the expression of P0 mRNA and P0 protein decreased in a dose-dependent manner after treatment with 2,5-HD for 4 weeks.Pretreatment with Egb761 protected against BNB interruption,and inhibited P0 mRNA and protein reduction during 2,5-HD treatment.Pretreatment with Egb761 significantly reduced loss of body weight (P＜0.01) and mitigated gait abnormalities (2.85±0.22) induced by 400 mg/kg 2,5-HD (P＜0.01).It also reduced the signs of neurotoxicity induced by 2,5-HD.Conclusion 2,5-HD inhibited the expression of P0 in a dose-dependent manner,and this may be an important mechanism by which toxic peripheral neuropathy is induced by 2,5-HD.Egb761 has a protective effect against 2,5-HD-induced peripheral neurotoxicity in rats.
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Abstract: Objective To investigate the role of myelin protein zero (P0) in 2,5-hexanedione (2,5-HD)-induced peripheral nerve injury,and the protective effect of Ginkgo biloba extract (Egb761) on 2,5-HD-induced toxic peripheral neuropathy.Methods After 4 weeks of treatment with 2,5-HD at different doses (50,100,200,400 mg/kg) in rats,changes in the levels of P0 in rat sciatic nerves was investigated,and the effect of Egb761 on 2,5-HD-induced toxic peripheral neuropathy was studied.Results The blood-nerve barrier (BNB) permeability of the sciatic nerve increased,and the expression of P0 mRNA and P0 protein decreased in a dose-dependent manner after treatment with 2,5-HD for 4 weeks.Pretreatment with Egb761 protected against BNB interruption,and inhibited P0 mRNA and protein reduction during 2,5-HD treatment.Pretreatment with Egb761 significantly reduced loss of body weight (P＜0.01) and mitigated gait abnormalities (2.85±0.22) induced by 400 mg/kg 2,5-HD (P＜0.01).It also reduced the signs of neurotoxicity induced by 2,5-HD.Conclusion 2,5-HD inhibited the expression of P0 in a dose-dependent manner,and this may be an important mechanism by which toxic peripheral neuropathy is induced by 2,5-HD.Egb761 has a protective effect against 2,5-HD-induced peripheral neurotoxicity in rats.
- Myelin protein zero /
- 2,5-hexanedione /
- Ginkgo biloba extract

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doi: 10.3967/0895-3988.2011.04.008

The Effect of 2,5-hexanedione on Myelin Protein Zero Expression, and Its Mitigation Using Ginkgo Biloba Extract

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The Effect of 2,5-hexanedione on Myelin Protein Zero Expression, and Its Mitigation Using Ginkgo Biloba Extract

doi: 10.3967/0895-3988.2011.04.008

English Abstract

The Effect of 2,5-hexanedione on Myelin Protein Zero Expression, and Its Mitigation Using Ginkgo Biloba Extract

National Institute for Occupational Health and Poison Control and Key laboratory of Chemical Safety and Health,Chinese Center for Disease Control and Prevention, Beijing 100050, China

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doi: 10.3967/0895-3988.2011.04.008

The Effect of 2,5-hexanedione on Myelin Protein Zero Expression, and Its Mitigation Using Ginkgo Biloba Extract

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The Effect of 2,5-hexanedione on Myelin Protein Zero Expression, and Its Mitigation Using Ginkgo Biloba Extract

doi: 10.3967/0895-3988.2011.04.008

English Abstract

The Effect of 2,5-hexanedione on Myelin Protein Zero Expression, and Its Mitigation Using Ginkgo Biloba Extract

National Institute for Occupational Health and Poison Control and Key laboratory of Chemical Safety and Health,Chinese Center for Disease Control and Prevention, Beijing 100050, China

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