QIU LianBo; CHEN Chen; DING GuiRong; ZHOU Yan; ZHANG MengYao

doi:10.3967/0895-3988.2011.04.016

The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(4): 438-444

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao. The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats[J]. Biomedical and Environmental Sciences, 2011, 24(4): 438-444. doi: 10.3967/0895-3988.2011.04.016

Citation:

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao. The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats[J]. Biomedical and Environmental Sciences, 2011, 24(4): 438-444. doi: 10.3967/0895-3988.2011.04.016

doi: 10.3967/0895-3988.2011.04.016

基金项目: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

Department of Blood Transfusion, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi,China
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shannxi, China
Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, Shannxi, China

Funds: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

摘要: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.
- /
- /
- /
- /
Abstract: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.
- Electromagnetic pulse /
- Tight junction /
- ZO-1 /
- Occludin /
- Actin

[1]	. 2024-1 Cover . Biomedical and Environmental Sciences, 2024, 37(1): -.
[2]	. 2024-1 Contents . Biomedical and Environmental Sciences, 2024, 37(1): 1-2.
[3]	Ruchi Tripathi, Sanjay Kumar Banerjee, Jay Prakash Nirala, Rajani Mathur. Exposure to Electromagnetic Fields from Mobile Phones and Fructose consumption Coalesce to Perturb Metabolic Regulators AMPK/SIRT1-UCP2/FOXO1 in Growing Rats . Biomedical and Environmental Sciences, 2023, 36(11): 1045-1058. doi: 10.3967/bes2023.134
[4]	LIU Han Rui, LI Chen Yang, XIA Xue, CHEN Shu Feng, LU Xiang Feng, GU Dong Feng, LIU Fang Chao, HUANG Jian Feng. Association of Estimated Pulse Wave Velocity and the Dynamic Changes in Estimated Pulse Wave Velocity with All-Cause Mortality among Middle-Aged and Elderly Chinese . Biomedical and Environmental Sciences, 2022, 35(11): 1001-1011. doi: 10.3967/bes2022.129
[5]	LI Yun Xiao, WANG Jun, WU Bo, LIN Fang, TAN Chun Ting, YU Gang Gang, NIE Shan, ZHAO Ran Ran, XU Bo. Effect of Maximal Oxygen Pulse on Patients with Chronic Obstructive Pulmonary Disease . Biomedical and Environmental Sciences, 2022, 35(9): 830-841. doi: 10.3967/bes2022.107
[6]	Ahmad Yahyazadeh, Berrin Zuhal Altunkaynak. Effect of Luteolin on Biochemical, Immunohistochemical, and Morphometrical Changes in Rat Spinal Cord following Exposure to a 900 MHz Electromagnetic Field . Biomedical and Environmental Sciences, 2020, 33(8): 593-602. doi: 10.3967/bes2020.078
[7]	Afolabi Olakunle Bamikole, Obajuluwa Adejoke Olukayode, Tiwa Obajuluwa, Okiki Pius, Oloyede Omotade Ibidun, Fadaka Oluwaseun Adewale, Ojo Oluwafemi Adeleke. Exposure to a 2.5 GHz Non-ionizing Electromagnetic Field Alters Hematological Profiles, Biochemical Parameters, and Induces Oxidative Stress in Male Albino Rats . Biomedical and Environmental Sciences, 2019, 32(11): 860-863. doi: 10.3967/bes2019.107
[8]	HAO Yan Hui, ZHAO Li, PENG Rui Yun. Effects of Electromagnetic Radiation on Autophagy and its Regulation . Biomedical and Environmental Sciences, 2018, 31(1): 57-65. doi: 10.3967/bes2018.006
[9]	Soonho Lim, Soo-Chan Kim, Jae Young Kim. Protective Effect of 10-Hz, 1-mT Electromagnetic Field Exposure Against Hypoxia/Reoxygenation Injury in HK-2 Cells . Biomedical and Environmental Sciences, 2015, 28(3): 231-234. doi: 10.3967/bes2015.032
[10]	DENG Xin Ru, ZHANG Yin Fei, WANG Tian Ge, XU Bai Hui, SUN Ji Chao, ZHAO Lie Bin, XU Min, CHEN Yu Hong, WANG Wei Qing, BI Yu Fang, LU Jie Li. Serum Calcium Level is Associated with Brachial-ankle Pulse Wave Velocity in Middle-aged and Elderly Chinese . Biomedical and Environmental Sciences, 2014, 27(8): 594-600. doi: 10.3967/bes2014.091
[11]	GENG Zhen, HOU Xue Xia, HAO Qin, ZHOU Hai Jian, WANG Feng, WAN Kang Lin. Optimization of Pulse-Field Gel Electrophoresis for Borrelia burgdorferi Subtyping . Biomedical and Environmental Sciences, 2013, 26(7): 584-591. doi: 10.3967/0895-3988.2013.07.010
[12]	ZHOU Jia Xing, DING Gui Rong, ZHANG Jie, ZHOU Yong Chun, ZHANG Yan Jun, GUO Guo Zhen. Detrimental Effect of Electromagnetic Pulse Exposure on Permeability of In Vitro Blood-brain-barrier Model . Biomedical and Environmental Sciences, 2013, 26(2): 128-137. doi: 10.3967/0895-3988.2013.02.007
[13]	ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng. Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change . Biomedical and Environmental Sciences, 2012, 25(2): 197-202. doi: 10.3967/0895-3988.2012.02.011
[14]	QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses . Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016
[15]	LAURA CONTALBRIGO, CALOGERO STELLETTA, LAURA FALCIONI, STEFANIA CASELLA, GIUSEPPE PICCION, MORANDO SOFFRITTI, MASSIMO MORGANTE. Effects of Different Electromagnetic Fields on Circadian Rhythms of Some Haematochemical Parameters in Rats . Biomedical and Environmental Sciences, 2009, 22(4): 348-353.
[16]	EKREM CICEK, OSMAN GOKALP, REMZI VAROL, GOKHAN CESUR. Influence of Electromagnetic Fields on Bone Fracture in Rats: Role of CAPE . Biomedical and Environmental Sciences, 2009, 22(2): 157-160.
[17]	GUI-RONG DING, KANG-CHU LI, XIAO-WU WANG, YONG-CHUN ZHOU, LIAN-BO QIU, JUAN TAN, SHENG-LONG XU, GUO-ZHEN GUO. Effect of Electromagnetic Pulse Exposure on Brain Micro Vascular Permeability in Rats . Biomedical and Environmental Sciences, 2009, 22(3): 265-268.
[18]	KANG-CHU LI, SHI-RONG MA, GUI-RONG DING, YAO GUO, GUO-ZHEN GUO. Effects of Electromagnetic Pulse on Bone Metabolism of Mice in vivo . Biomedical and Environmental Sciences, 2009, 22(6): 518-521.
[19]	XIAO-WU WANG, GUI-RONG DING, CHANG-HONG SHI, TAO ZHAO, JIE ZHANG, LI-HUA ZENG, GUO-ZHEN GUO. Effect of Electromagnetic Pulse Exposure on Permeability of Blood-testicle Barrier in Mice . Biomedical and Environmental Sciences, 2008, 21(3): 218-221.
[20]	ARCHANA AMBA, MANOJ KUMAR, R.K.UPRETI, SUBHASH K.KHANNA, MUKUL DAS. Effect of Dietary Administration of Lathyrus sativus Pulse on Intestinal Biochemical Parameters in Normal and Scorbutic Guinea Pigs . Biomedical and Environmental Sciences, 2002, 15(4): 315-322.

点击查看大图

计量

文章访问数: 1241
HTML全文浏览量: 338
PDF下载量: 33
被引次数: 0

The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

doi: 10.3967/0895-3988.2011.04.016

基金项目: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

刊出日期: 2011-08-20

关键词:

Electromagnetic pulse /

Tight junction /

ZO-1 /

Occludin /

Actin

摘要: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.

English Abstract

The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats

Department of Blood Transfusion, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shannxi,China

Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shannxi, China

Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, Shannxi, China

Funds: the Research Fund of Scientific and technological research and Development Program of Shannxi province of China(2010K16-04-04)

Publish Date: 2011-08-20

Keywords:

Abstract: Objective To investigate changes in the expression of tight junction (TJ) proteins in the cerebral cortex,hippocampus,heart,lung,and testes of rats after exposure to electromagnetic pulse (EMP).Methods Eighteen adult male Sprague-Dawley rats were divided into sham and exposure groups.The exposure groups received EMP at 200 kV/m for 200 pulses with a repetition rate of 1 Hz.The expression of TJ proteins (ZO-1,occludin,actin) in the several organs was examined by western blotting.Results ZO-1 levels in the cerebral cortex decreased 1 h and 3 h after EMP exposure compared with sham group (P＜0.05).No significant difference was observed for occludin and actin.ZO-1 levels in the hippocampus increased 1 h and 3 h post-exposure (P＜0.05),and occludin decreased after 3 h (P＜0.05);however,actin was unaffected.ZO-1 levels in the heart increased 3 h post-exposure (P＜0.05),occludin decreased 3 h post-exposure (P＜0.05),and actin increased 1 h and 3 h post-exposure (P＜0.05).ZO-1,occludin and actin levels in the lung decreased compared with those in the sham group (P＜0.05).ZO-1 and occludin levels in the testes decreased 1 h and 3 h post-exposure (P＜0.05),but actin showed no significant change.Conclusion Exposure to EMP altered the expression levels of TJ proteins,particularly ZO-1,in the organs of adult male rats,which may induce changes in barrier structure and function.

Citation:

QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao. The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats[J]. Biomedical and Environmental Sciences, 2011, 24(4): 438-444. doi: 10.3967/0895-3988.2011.04.016