ZHANG Ya Mei; ZHOU Yan; QIU Lian Bo; DING Gui Rong; PANG Xiao Feng

doi:10.3967/0895-3988.2012.02.011

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(2): 197-202

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng. Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change[J]. Biomedical and Environmental Sciences, 2012, 25(2): 197-202. doi: 10.3967/0895-3988.2012.02.011

Citation:

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng. Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change[J]. Biomedical and Environmental Sciences, 2012, 25(2): 197-202. doi: 10.3967/0895-3988.2012.02.011

doi: 10.3967/0895-3988.2012.02.011

基金项目: The National Key Basic Research and Development Plan of China(212011CB503701)

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China
Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China

Funds: The National Key Basic Research and Development Plan of China(212011CB503701)

摘要: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.
- /
- /
Abstract: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.
- Pulse electromagnetic field /
- Blood-brain barrier /
- Cerebral microvasculature

[1]	YANG Zuo Sen, BAI Ying Long, JIN Cui Hong, NA Jun, ZHANG Rui, GAO Yuan, PAN Guo Wei, YAN Ling Jun, SUN Wei. Evidence on Invasion of Blood, Adipose Tissues, Nervous System and Reproductive System of Mice After a Single Oral Exposure: Nanoplastics versus Microplastics . Biomedical and Environmental Sciences, 2022, 35(11): 1025-1037. doi: 10.3967/bes2022.131
[2]	Ahmad Yahyazadeh, Berrin Zuhal Altunkaynak. Effect of Luteolin on Biochemical, Immunohistochemical, and Morphometrical Changes in Rat Spinal Cord following Exposure to a 900 MHz Electromagnetic Field . Biomedical and Environmental Sciences, 2020, 33(8): 593-602. doi: 10.3967/bes2020.078
[3]	ZHAO Kang Feng, SONG Yu Qing, ZHANG Rui Hua, YANG Xiao Yan, SUN Bo, HOU Zhi Quan, PU Xiao Ping, DAI Hong Xing, BAI Xue Tao. Comparative Toxicity of Nanomaterials to Air-blood Barrier Permeability Using an In Vitro Model . Biomedical and Environmental Sciences, 2019, 32(8): 602-613. doi: 10.3967/bes2019.078
[4]	Afolabi Olakunle Bamikole, Obajuluwa Adejoke Olukayode, Tiwa Obajuluwa, Okiki Pius, Oloyede Omotade Ibidun, Fadaka Oluwaseun Adewale, Ojo Oluwafemi Adeleke. Exposure to a 2.5 GHz Non-ionizing Electromagnetic Field Alters Hematological Profiles, Biochemical Parameters, and Induces Oxidative Stress in Male Albino Rats . Biomedical and Environmental Sciences, 2019, 32(11): 860-863. doi: 10.3967/bes2019.107
[5]	ZHAO Qi, YANG Wen Li, WANG Xiao Na, Wang Ruikang K, YOU Qi Sheng, CHU Zhong Di, XIN Chen, ZHANG Meng Yu, LI Dong Jun, WANG Zi Yang, CHEN Wei, LI Yi Feng, CUI Rui, SHEN Lin, WEI Wen Bin. Repeatability and Reproducibility of Quantitative Assessment of the Retinal Microvasculature Using Optical Coherence Tomography Angiography Based on Optical Microangiography . Biomedical and Environmental Sciences, 2018, 31(6): 407-412. doi: 10.3967/bes2018.054
[6]	ZHANG Bin, SHAO Hua, WANG Xiu Hui, CHEN Xiao, LI Zhong Sheng, CAO Peng, ZHU Dan, YANG Yi Guang, XIAO Jing Wei, LI Bin. Acrylamide-induced Subacute Neurotoxic Effects on the Cerebral Cortex and Cerebellum at the Synapse Level in Rats . Biomedical and Environmental Sciences, 2017, 30(6): 432-443. doi: 10.3967/bes2017.057
[7]	Soonho Lim, Soo-Chan Kim, Jae Young Kim. Protective Effect of 10-Hz, 1-mT Electromagnetic Field Exposure Against Hypoxia/Reoxygenation Injury in HK-2 Cells . Biomedical and Environmental Sciences, 2015, 28(3): 231-234. doi: 10.3967/bes2015.032
[8]	AN Dai Zhi, AI Jun Tao, FANG Hong Juan, SUN Ru Bao, SHI Yun, WANG Li Li, WANG Qiang. Influence of Iron Supplementation on DMT1 (IRE)-induced Transport of Lead by Brain Barrier Systems in vivo . Biomedical and Environmental Sciences, 2015, 28(9): 651-659. doi: 10.3967/bes2015.091
[9]	GENG Zhen, HOU Xue Xia, HAO Qin, ZHOU Hai Jian, WANG Feng, WAN Kang Lin. Optimization of Pulse-Field Gel Electrophoresis for Borrelia burgdorferi Subtyping . Biomedical and Environmental Sciences, 2013, 26(7): 584-591. doi: 10.3967/0895-3988.2013.07.010
[10]	ZHOU Jia Xing, DING Gui Rong, ZHANG Jie, ZHOU Yong Chun, ZHANG Yan Jun, GUO Guo Zhen. Detrimental Effect of Electromagnetic Pulse Exposure on Permeability of In Vitro Blood-brain-barrier Model . Biomedical and Environmental Sciences, 2013, 26(2): 128-137. doi: 10.3967/0895-3988.2013.02.007
[11]	YUAN Yan, BIAN Jian Chun, LIU Xue Zhong, ZHANG Ying, SUN Ya, LIU Zong Ping. Oxidative Stress and Apoptotic Changes of Rat Cerebral Cortical NeuronsExposed to Cadmium in Vitro . Biomedical and Environmental Sciences, 2012, 25(2): 172-181. doi: 10.3967/0895-3988.2012.02.008
[12]	QIU LianBo, CHEN Chen, DING GuiRong, ZHOU Yan, ZHANG MengYao. The Effects of Electromagnetic Pulse on the Protein Levels of Tight Junction Associated-Proteins in the Cerebral Cortex, Hippocampus, Heart, Lung, and Testis of Rats . Biomedical and Environmental Sciences, 2011, 24(4): 438-444. doi: 10.3967/0895-3988.2011.04.016
[13]	KANG-CHU LI, SHI-RONG MA, GUI-RONG DING, YAO GUO, GUO-ZHEN GUO. Effects of Electromagnetic Pulse on Bone Metabolism of Mice in vivo . Biomedical and Environmental Sciences, 2009, 22(6): 518-521.
[14]	GUI-RONG DING, KANG-CHU LI, XIAO-WU WANG, YONG-CHUN ZHOU, LIAN-BO QIU, JUAN TAN, SHENG-LONG XU, GUO-ZHEN GUO. Effect of Electromagnetic Pulse Exposure on Brain Micro Vascular Permeability in Rats . Biomedical and Environmental Sciences, 2009, 22(3): 265-268.
[15]	XIAO-WU WANG, GUI-RONG DING, CHANG-HONG SHI, TAO ZHAO, JIE ZHANG, LI-HUA ZENG, GUO-ZHEN GUO. Effect of Electromagnetic Pulse Exposure on Permeability of Blood-testicle Barrier in Mice . Biomedical and Environmental Sciences, 2008, 21(3): 218-221.
[16]	FU-CHUN JING, HONG CHEN, CHANG-LING LI. Rapid Determination of Dopamine and Its Metabolites During in vivo Cerebral Microdialysis by Routine High Performance Liquid Chromatography With Electrochemical Detection . Biomedical and Environmental Sciences, 2007, 20(4): 317-320.
[17]	XIAO-HONG XU, XIAO-XIANG ZHENG, QIONG ZHOU, HUI LI. Inhibition of Excitatory Amino Acid Efflux Contributes to Protective Effects of Puerarin Against Cerebral Ischemia in Rats . Biomedical and Environmental Sciences, 2007, 20(4): 336-342.
[18]	XIAO-CHAO CHEN, MING-TONG XU, WU ZHOU, CHUN-LI HAN, WEI-QING CHEN. A Meta-analysis of β-fibrinogen Gene-455G/A Polymorphism and Plasma Fibrinogen Level in Chinese Cerebral Infarction Patients . Biomedical and Environmental Sciences, 2007, 20(5): 366-372.
[19]	HONG WAN, SHAO-DONG ZHANG, JUN-HUA LI. Action of Schwann Cells Implanted in Cerebral Hemorrhage Lesion . Biomedical and Environmental Sciences, 2007, 20(1): 47-51.
[20]	CHEN HUAI_HONG, ZHOU JNU-FU. Low Cholesterol in Erythrocyte Membranes and High Lipoperoxides in Erythrocytes Are the Potential Risk Factors for Cerebral Hemorrhagic Stroke in Human . Biomedical and Environmental Sciences, 2001, 14(3): 189-198.

点击查看大图

计量

文章访问数: 1621
HTML全文浏览量: 506
PDF下载量: 76
被引次数: 0

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

doi: 10.3967/0895-3988.2012.02.011

基金项目: The National Key Basic Research and Development Plan of China(212011CB503701)

刊出日期: 2012-04-20

关键词:

Pulse electromagnetic field /

Blood-brain barrier /

Cerebral microvasculature

摘要: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.

English Abstract

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China

Funds: The National Key Basic Research and Development Plan of China(212011CB503701)

Publish Date: 2012-04-20

Keywords:

Abstract: Objective To investigate the expression of occludin,ZO-1,MMP-2,and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.Methods Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n=8).After exposure to PEMF at 0.5,1,3,6,and 12 h,BBB permeability was measured by Evans-Blue extravasation.The expression of occludin,ZO-1,MMP-2,and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting.MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.Results Compared with the sham group,PEMF exposure led to increased permeability of the BBB to EB,which was prolonged after exposure.BBB permeability became progressively more severe,and recovered at 6 h.The gene and protein expression of occludin and ZO-1 were significantly decreased,while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF.All levels of expression recovered 12 h following PEMF.Conclusion Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.

Citation:

ZHANG Ya Mei, ZHOU Yan, QIU Lian Bo, DING Gui Rong, PANG Xiao Feng. Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change[J]. Biomedical and Environmental Sciences, 2012, 25(2): 197-202. doi: 10.3967/0895-3988.2012.02.011

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/0895-3988.2012.02.011

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

计量

出版历程

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

doi: 10.3967/0895-3988.2012.02.011

English Abstract

Altered Expression of Matrix Metalloproteinases and Tight Junction Proteins in Rats following PEMF-Induced BBB Permeability Change

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China

全文HTML

目录

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China

Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, Shanxi, China