GONG Han Shi; TIAN Chan; ZHANG Bao Yurn; WANG Zhao Yun; XIE Wu Ling; JING Yuan Yuan; GAO Chen; JIANG Hui Ying; SHI Qi; LIU Yong; DONG Xiao Ping

doi:10.3967/0895-3988.2011.06.004

doi: 10.3967/0895-3988.2011.06.004

基金项目: Chinese National Natural Science Foundation(30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101)%the SKLID Development Grant(2008SKLID102%2008SKLID202)

Establishment of Hamster- and Human-PRNP Transgenic Mice

School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China

Funds: Chinese National Natural Science Foundation(30771914%30800975)%Institution Technique R&D Grant(2008EG150300)%National Basic Research Program of China (973 Program)(2007CB310505)%China Mega-Project for Infectious Disease(2009ZX10004-101)%the SKLID Development Grant(2008SKLID102%2008SKLID202)

摘要: Objective To create transgenic mice expressing hamster- and human-PRNP as a model for understanding the physiological function and pathology of prion protein (PrP),as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies (TSEs).Methods Hamster and human-PRNP transgenic mice were established by conventional methods.The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR.PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR,real-time RT-PCR,and western blot analysis.Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.Results Integrated PRNP copy number in various mouse lines was 53 (Tg-haPrP1),18 (Tg-huPrP1),3 (Tg-huPrP2),and 16 (Tg-huPrP5),respectively.Exogenous PrPs were expressed at both the transcriptional and translational level.Histological assays did not detect any abnormalities in brain or other organs.Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines.These four transgenic mouse lines provide ideal models for additional research.
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Abstract: Objective To create transgenic mice expressing hamster- and human-PRNP as a model for understanding the physiological function and pathology of prion protein (PrP),as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies (TSEs).Methods Hamster and human-PRNP transgenic mice were established by conventional methods.The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR.PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR,real-time RT-PCR,and western blot analysis.Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.Results Integrated PRNP copy number in various mouse lines was 53 (Tg-haPrP1),18 (Tg-huPrP1),3 (Tg-huPrP2),and 16 (Tg-huPrP5),respectively.Exogenous PrPs were expressed at both the transcriptional and translational level.Histological assays did not detect any abnormalities in brain or other organs.Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines.These four transgenic mouse lines provide ideal models for additional research.
- PrP /
- PRNP /
- Transgenic mice /
- Copy number

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doi: 10.3967/0895-3988.2011.06.004

Establishment of Hamster- and Human-PRNP Transgenic Mice

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doi: 10.3967/0895-3988.2011.06.004

Establishment of Hamster- and Human-PRNP Transgenic Mice

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Establishment of Hamster- and Human-PRNP Transgenic Mice

doi: 10.3967/0895-3988.2011.06.004

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Establishment of Hamster- and Human-PRNP Transgenic Mice

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