LI Qiao; ZHANG Shu Hua; YU Yuan Hua; WANG Li Ping; GUAN Shu Wen; LI Peng Fei

doi:10.3967/0895-3988.2012.02.014

Toxicity of Sodium Fluoride to Caenorhabditis elegans

LI Qiao, ZHANG Shu Hua, YU Yuan Hua, WANG Li Ping, GUAN Shu Wen, LI Peng Fei

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(2): 216-223

LI Qiao, ZHANG Shu Hua, YU Yuan Hua, WANG Li Ping, GUAN Shu Wen, LI Peng Fei. Toxicity of Sodium Fluoride to Caenorhabditis elegans[J]. Biomedical and Environmental Sciences, 2012, 25(2): 216-223. doi: 10.3967/0895-3988.2012.02.014

Citation:

LI Qiao, ZHANG Shu Hua, YU Yuan Hua, WANG Li Ping, GUAN Shu Wen, LI Peng Fei. Toxicity of Sodium Fluoride to Caenorhabditis elegans[J]. Biomedical and Environmental Sciences, 2012, 25(2): 216-223. doi: 10.3967/0895-3988.2012.02.014

doi: 10.3967/0895-3988.2012.02.014

基金项目: Jilin Provincial Science and Technology Development Project in China(20100407)

Toxicity of Sodium Fluoride to Caenorhabditis elegans

Life Science and Technology Institute, Changchun University of Science and Technology, Changchun 130022,Jilin, China
College of Life Science, Jilin University, Changchun 130021, Jilin, China

Funds: Jilin Provincial Science and Technology Development Project in China(20100407)

摘要: Objective To investigate the toxic effect of sodium fluoride (NaF) on the nematode Caenorhabditis elegans (C.elegans).Methods Adult C.elegans were exposed to different concentrations of NaF (0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L) for 24 h.To assess the physiological effects of NaF,the brood size,life span,head thrashes,and body bend frequency were examined.Reactive oxygen species (ROS) and cell apoptosis were detected as parameters of biochemical response.The gene expressions were determined by real-time polymerase chain reaction (PCR) to assess the molecular-level response.Results At the physiological level,the brood size of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 6％,26％,and 28％ respectively in comparison with the control group.The maximum life spans of C.e/elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 3 days and 5 days,respectively.Head thrashes and body bend frequency both decreased with increasing concentrations of NaF.At the biochemical level,the production of ROS and the incidence of cell apoptosis increased with increasing concentrations of NaF(P＜0.05).At the molecular level,different concentrations of NaF exposure raised the expression of stress-related genes,such as hsp16.1,sod-3,ctl-2,dhs-28,gst-1,and cep-1.Conclusion NaF exposure could induce multiple biological toxicities to C.elegans in a concentration-dependent manner.These toxicities may be relevant to the oxidative stress induced by increased ROS production and accumulation in C.elegans.
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Abstract: Objective To investigate the toxic effect of sodium fluoride (NaF) on the nematode Caenorhabditis elegans (C.elegans).Methods Adult C.elegans were exposed to different concentrations of NaF (0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L) for 24 h.To assess the physiological effects of NaF,the brood size,life span,head thrashes,and body bend frequency were examined.Reactive oxygen species (ROS) and cell apoptosis were detected as parameters of biochemical response.The gene expressions were determined by real-time polymerase chain reaction (PCR) to assess the molecular-level response.Results At the physiological level,the brood size of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 6％,26％,and 28％ respectively in comparison with the control group.The maximum life spans of C.e/elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 3 days and 5 days,respectively.Head thrashes and body bend frequency both decreased with increasing concentrations of NaF.At the biochemical level,the production of ROS and the incidence of cell apoptosis increased with increasing concentrations of NaF(P＜0.05).At the molecular level,different concentrations of NaF exposure raised the expression of stress-related genes,such as hsp16.1,sod-3,ctl-2,dhs-28,gst-1,and cep-1.Conclusion NaF exposure could induce multiple biological toxicities to C.elegans in a concentration-dependent manner.These toxicities may be relevant to the oxidative stress induced by increased ROS production and accumulation in C.elegans.
- Sodium fluoride /
- Caenorhabditis elegans /
- Toxicity /
- Real-time PCR

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Toxicity of Sodium Fluoride to Caenorhabditis elegans

doi: 10.3967/0895-3988.2012.02.014

基金项目: Jilin Provincial Science and Technology Development Project in China(20100407)

刊出日期: 2012-04-20

关键词:

Sodium fluoride /

Caenorhabditis elegans /

Toxicity /

Real-time PCR

摘要: Objective To investigate the toxic effect of sodium fluoride (NaF) on the nematode Caenorhabditis elegans (C.elegans).Methods Adult C.elegans were exposed to different concentrations of NaF (0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L) for 24 h.To assess the physiological effects of NaF,the brood size,life span,head thrashes,and body bend frequency were examined.Reactive oxygen species (ROS) and cell apoptosis were detected as parameters of biochemical response.The gene expressions were determined by real-time polymerase chain reaction (PCR) to assess the molecular-level response.Results At the physiological level,the brood size of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 6％,26％,and 28％ respectively in comparison with the control group.The maximum life spans of C.e/elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 3 days and 5 days,respectively.Head thrashes and body bend frequency both decreased with increasing concentrations of NaF.At the biochemical level,the production of ROS and the incidence of cell apoptosis increased with increasing concentrations of NaF(P＜0.05).At the molecular level,different concentrations of NaF exposure raised the expression of stress-related genes,such as hsp16.1,sod-3,ctl-2,dhs-28,gst-1,and cep-1.Conclusion NaF exposure could induce multiple biological toxicities to C.elegans in a concentration-dependent manner.These toxicities may be relevant to the oxidative stress induced by increased ROS production and accumulation in C.elegans.

English Abstract

Toxicity of Sodium Fluoride to Caenorhabditis elegans

Life Science and Technology Institute, Changchun University of Science and Technology, Changchun 130022,Jilin, China

College of Life Science, Jilin University, Changchun 130021, Jilin, China

Funds: Jilin Provincial Science and Technology Development Project in China(20100407)

Publish Date: 2012-04-20

Keywords:

Abstract: Objective To investigate the toxic effect of sodium fluoride (NaF) on the nematode Caenorhabditis elegans (C.elegans).Methods Adult C.elegans were exposed to different concentrations of NaF (0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L) for 24 h.To assess the physiological effects of NaF,the brood size,life span,head thrashes,and body bend frequency were examined.Reactive oxygen species (ROS) and cell apoptosis were detected as parameters of biochemical response.The gene expressions were determined by real-time polymerase chain reaction (PCR) to assess the molecular-level response.Results At the physiological level,the brood size of C.elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 6％,26％,and 28％ respectively in comparison with the control group.The maximum life spans of C.e/elegans exposed to 0.038 mmol/L,0.38 mmol/L,and 3.8 mmol/L concentrations of NaF were reduced by 3 days and 5 days,respectively.Head thrashes and body bend frequency both decreased with increasing concentrations of NaF.At the biochemical level,the production of ROS and the incidence of cell apoptosis increased with increasing concentrations of NaF(P＜0.05).At the molecular level,different concentrations of NaF exposure raised the expression of stress-related genes,such as hsp16.1,sod-3,ctl-2,dhs-28,gst-1,and cep-1.Conclusion NaF exposure could induce multiple biological toxicities to C.elegans in a concentration-dependent manner.These toxicities may be relevant to the oxidative stress induced by increased ROS production and accumulation in C.elegans.

Citation: