WEI Li Li; CHEN Hong; JIANG Yong; TU Peng Fei; ZHONG Ming; DU Juan; LIU Fei; WANG Lei; LIU Chun Yang

doi:10.3967/0895-3988.2012.02.017

Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats*

WEI Li Li, CHEN Hong, JIANG Yong, TU Peng Fei, ZHONG Ming, DU Juan, LIU Fei, WANG Lei, LIU Chun Yang

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(2): 238-244

WEI Li Li, CHEN Hong, JIANG Yong, TU Peng Fei, ZHONG Ming, DU Juan, LIU Fei, WANG Lei, LIU Chun Yang. Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats*[J]. Biomedical and Environmental Sciences, 2012, 25(2): 238-244. doi: 10.3967/0895-3988.2012.02.017

Citation:

WEI Li Li, CHEN Hong, JIANG Yong, TU Peng Fei, ZHONG Ming, DU Juan, LIU Fei, WANG Lei, LIU Chun Yang. Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats*[J]. Biomedical and Environmental Sciences, 2012, 25(2): 238-244. doi: 10.3967/0895-3988.2012.02.017

doi: 10.3967/0895-3988.2012.02.017

基金项目: the National Natural Science Foundation of China(30560171%30860334)

Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats*

Ministry of Education Key Laboratory of Xinjiang Phytomedicine Resources, Shihezi University, Shihezi 832002, Xinjiang, China;Department of Pharmacology, College of Pharmacy, Shihezi University, Shihezi 832002, Xinjiang, China
Peking University Modern Research Center for Traditional Chinese Medicine,Beijing 100083, China

Funds: the National Natural Science Foundation of China(30560171%30860334)

摘要: Objective To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine (NE),dopamine (DA),homovanillic acid (HVA),3,4-dihydroxyphenylethanoid acid (DOPAC),5-hydroxyindoleacetic acid (HIAA),and 5-hydroxytryptamine(5-HT) in middle cerebral artery occlusion (MCAO rats.Methods The middle cerebral artery was occluded in male Sprague-Dawley rats.Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer's solution at a rate of 1.5 μL/min.Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE,DA,HVA,DOPAC,HIAA,and 5-HT levels by reverse phase HPLC with electrochemistry.Results Three days after MCAO,the extracellular striatal levels of NE,DA,DOPAC,HIAA,HVA,and 5-HT of the MCAO rats increased significantly (at least P＜0.05 vs.control).However,simultaneous treatment with echinacoside (30.0 or 15.0 mg/kg) attenuated these increases (at least P＜0.05 vs.non-treated model rats).Conclusion These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.
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Abstract: Objective To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine (NE),dopamine (DA),homovanillic acid (HVA),3,4-dihydroxyphenylethanoid acid (DOPAC),5-hydroxyindoleacetic acid (HIAA),and 5-hydroxytryptamine(5-HT) in middle cerebral artery occlusion (MCAO rats.Methods The middle cerebral artery was occluded in male Sprague-Dawley rats.Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer's solution at a rate of 1.5 μL/min.Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE,DA,HVA,DOPAC,HIAA,and 5-HT levels by reverse phase HPLC with electrochemistry.Results Three days after MCAO,the extracellular striatal levels of NE,DA,DOPAC,HIAA,HVA,and 5-HT of the MCAO rats increased significantly (at least P＜0.05 vs.control).However,simultaneous treatment with echinacoside (30.0 or 15.0 mg/kg) attenuated these increases (at least P＜0.05 vs.non-treated model rats).Conclusion These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.

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Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats*

doi: 10.3967/0895-3988.2012.02.017

基金项目: the National Natural Science Foundation of China(30560171%30860334)

刊出日期: 2012-04-20

关键词:

Echinacoside /

Norepinephrine (NE) dopamine (DA) homovanillic acid (HVA) 3,4-dihydroxyphenylethanoid acid (DOPAC) 5-hydroxyindoleacetic acid (HIAA) 5-hydroxytryptamine (5-HT) /

Middle cerebral artery occlusion(MCAO) /

Brain microdialysis /

rats

摘要: Objective To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine (NE),dopamine (DA),homovanillic acid (HVA),3,4-dihydroxyphenylethanoid acid (DOPAC),5-hydroxyindoleacetic acid (HIAA),and 5-hydroxytryptamine(5-HT) in middle cerebral artery occlusion (MCAO rats.Methods The middle cerebral artery was occluded in male Sprague-Dawley rats.Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer's solution at a rate of 1.5 μL/min.Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE,DA,HVA,DOPAC,HIAA,and 5-HT levels by reverse phase HPLC with electrochemistry.Results Three days after MCAO,the extracellular striatal levels of NE,DA,DOPAC,HIAA,HVA,and 5-HT of the MCAO rats increased significantly (at least P＜0.05 vs.control).However,simultaneous treatment with echinacoside (30.0 or 15.0 mg/kg) attenuated these increases (at least P＜0.05 vs.non-treated model rats).Conclusion These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.

English Abstract

Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats*

Ministry of Education Key Laboratory of Xinjiang Phytomedicine Resources, Shihezi University, Shihezi 832002, Xinjiang, China;Department of Pharmacology, College of Pharmacy, Shihezi University, Shihezi 832002, Xinjiang, China

Peking University Modern Research Center for Traditional Chinese Medicine,Beijing 100083, China

Funds: the National Natural Science Foundation of China(30560171%30860334)

Publish Date: 2012-04-20

Keywords:

Abstract: Objective To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine (NE),dopamine (DA),homovanillic acid (HVA),3,4-dihydroxyphenylethanoid acid (DOPAC),5-hydroxyindoleacetic acid (HIAA),and 5-hydroxytryptamine(5-HT) in middle cerebral artery occlusion (MCAO rats.Methods The middle cerebral artery was occluded in male Sprague-Dawley rats.Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer's solution at a rate of 1.5 μL/min.Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE,DA,HVA,DOPAC,HIAA,and 5-HT levels by reverse phase HPLC with electrochemistry.Results Three days after MCAO,the extracellular striatal levels of NE,DA,DOPAC,HIAA,HVA,and 5-HT of the MCAO rats increased significantly (at least P＜0.05 vs.control).However,simultaneous treatment with echinacoside (30.0 or 15.0 mg/kg) attenuated these increases (at least P＜0.05 vs.non-treated model rats).Conclusion These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.

Citation:

WEI Li Li, CHEN Hong, JIANG Yong, TU Peng Fei, ZHONG Ming, DU Juan, LIU Fei, WANG Lei, LIU Chun Yang. Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats*[J]. Biomedical and Environmental Sciences, 2012, 25(2): 238-244. doi: 10.3967/0895-3988.2012.02.017