HAO Chang Fu; LI Xiao Fang; YAO Wu

doi:10.3967/bes2013.033

Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

HAO Chang Fu, LI Xiao Fang, YAO Wu

文章导航 > Biomedical and Environmental Sciences > 2013 > 26(12): 979-985

HAO Chang Fu, LI Xiao Fang, YAO Wu. Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts[J]. Biomedical and Environmental Sciences, 2013, 26(12): 979-985. doi: 10.3967/bes2013.033

Citation:

HAO Chang Fu, LI Xiao Fang, YAO Wu. Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts[J]. Biomedical and Environmental Sciences, 2013, 26(12): 979-985. doi: 10.3967/bes2013.033

doi: 10.3967/bes2013.033

基金项目: the Research Fund from the National Natural Science Foundation of China (#81102109)

Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

Department of 0ccupational Health and 0ccupational Disease, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China

Funds: the Research Fund from the National Natural Science Foundation of China (#81102109)

摘要: Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts
Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels ofα-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.
Results The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.
Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.
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Abstract: Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts
Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels ofα-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.
Results The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.
Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.
- Transdifferentiation /
- Lung fibroblasts /
- Insulin-like growth factor II receptor /
- Silicosis

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Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

doi: 10.3967/bes2013.033

基金项目: the Research Fund from the National Natural Science Foundation of China (#81102109)

刊出日期: 2013-12-20

关键词:

Transdifferentiation /

Lung fibroblasts /

Insulin-like growth factor II receptor /

Silicosis

摘要: Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts
Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels ofα-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.
Results The expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.
Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.