WANG An Na; WANG Quan Kai; YANG Min; HU Jie; DONG Lin; andXU Jian Ning

doi:10.3967/bes2014.002

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(7): 523-530

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning. Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation[J]. Biomedical and Environmental Sciences, 2014, 27(7): 523-530. doi: 10.3967/bes2014.002

Citation:

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning. Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation[J]. Biomedical and Environmental Sciences, 2014, 27(7): 523-530. doi: 10.3967/bes2014.002

doi: 10.3967/bes2014.002

基金项目: National Natural Science Foundation of China(81072318)

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

National Institute of 0ccupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Funds: National Natural Science Foundation of China(81072318)

摘要: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.
- /
- /
- /
Abstract: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.
- DNA methylation /
- Chip /
- Glycidyl methacrylate /
- 16HBE

[1]	ZHAO Lei, JIA Ya Ning, LIU Qi Si Jing, LIU Zi Quan, LIN Hui Shu, SHUI Xin Ying, GUO Li Qiong, HOU Shi Ke. Association between Mitochondrial DNA Methylation and Hypertension Risk: A Cross-sectional Study in Chinese Northern Population . Biomedical and Environmental Sciences, 2023, 36(10): 972-978. doi: 10.3967/bes2023.122
[2]	LI Zhi Yuan, GONG Yong Xiang, YANG Meng, CHAI Jian, SUN Ren Jie, LI Qin Yang, HE Ya Nan, HUANG Hui, ZHANG Ya Wei, BA Yue, ZHOU Guo Yu. Weather and Birth Weight: Different Roles of Maternal and Neonatal GPR61 Promoter Methylation . Biomedical and Environmental Sciences, 2022, 35(3): 181-193. doi: 10.3967/bes2022.027
[3]	CAI Ying, LIU Yan Ping, ZHENG Kai, LEI Yuan Di, WANG Ye, XU Xin Yun, ZHANG Zhao Hui. Proteomics Study on the Differentially Expressed Proteins in 16HBE Cells Exposed to Beryllium Sulfate . Biomedical and Environmental Sciences, 2021, 34(11): 926-930. doi: 10.3967/bes2021.128
[4]	WANG Bing Yu, CAI Ying, ZHENG Kai, HUANG Hai Yan, QIN Xiao Yun, XU Xin Yun. PM_2.5-induced Alterations of Gene Expression in HBE Cells Revealed by Gene Chip Analysis . Biomedical and Environmental Sciences, 2020, 33(3): 213-216. doi: 10.3967/bes2020.030
[5]	ZHANG Chun Hong, HUO Jun Sheng, CHEN Shan, XU You Chun, SUN Jing, HUANG Jian. Microfluidic Chip Method for Multi-SNPs Genotyping in Individual Risk Assessment of Micronutrient Deficiency . Biomedical and Environmental Sciences, 2019, 32(6): 471-475. doi: 10.3967/bes2019.061
[6]	YIN Ji Yong, HUO Jun Sheng, MA Xin Xin, SUN Jing, HUANG Jian. Study on the Simultaneously Quantitative Detection for β-Lactoglobulin and Lactoferrin of Cow Milk by Using Protein Chip Technique . Biomedical and Environmental Sciences, 2017, 30(12): 875-886. doi: 10.3967/bes2017.118
[7]	WU Jian Hui, CHEN Jiao, WANG Yong, XIA Bin, WANG Rong, ZHAO Yan, WANG Qin Xia, SONG Qi, YAO Shun Heng, ZHANG Yun Hui, SUN Zu Yue. Effect of Mono-2-ethyhexyl Phthalate on DNA Methylation in Human Prostate Cancer LNCaP Cells . Biomedical and Environmental Sciences, 2017, 30(9): 641-648. doi: 10.3967/bes2017.085
[8]	MAO Li Li, XIAO Xin Hua, ZHANG Qian, ZHENG Jia, LI Wen Hui, YU Miao, ZHANG Hua Bing, PING Fan, XU Jian Ping, WANG Xiao Jing. DNA Methylation and Birth Weight:a Genome-wide Analysis . Biomedical and Environmental Sciences, 2017, 30(9): 667-670. doi: 10.3967/bes2017.088
[9]	SHI Xiao Wei, SHI Bo Hui. Exploring Genome-wide DNA Methylation Profiles Altered in Kashin-Beck Disease Using Infinium Human Methylation 450 Bead Chips . Biomedical and Environmental Sciences, 2016, 29(7): 539-543. doi: 10.3967/bes2016.072
[10]	WANGFeiFei, GENGChunMei, HAO WeiDong, ZHAO YongDong, LIQin, WANG HongMei, QIAN Yan. The Cellular Toxicity of PM2.5 Emitted from Coal Combustion in Human Umbilical Vein Endothelial Cells . Biomedical and Environmental Sciences, 2016, 29(2): 107-116. doi: 10.3967/bes2016.012
[11]	ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun. Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats . Biomedical and Environmental Sciences, 2011, 24(2): 163-171. doi: 10.3967/0895-3988.2011.02.011
[12]	XIN-YU SHAO, RONG ZHANG, CHENG HU, CONG-RONG WANG, JING-YI LU, WEN QIN, HAO-YONG YU, YU-QIAN BAO, XING-BO CHENG, WEI-PING JIA. Precise Microdeletion Detection of Prader-Willi Syndrome with Array Comparative Genome Hybridization . Biomedical and Environmental Sciences, 2010, 23(3): 194-198.
[13]	YUE-LAN SHEN, YI-Guo JIANG, ANNE R. GREENLEE, LAN-LAN ZHOU, LIN-HUA LIU. MicroRNA Expression Profiles and MiR-10a Target in Anti-benzo[a] pyrene-7, 8-diol-9, 10-epoxide-transformed Human 16HBE Cells . Biomedical and Environmental Sciences, 2009, 22(1): 14-21.
[14]	HUA-JIE CHEN, RI-AN YU, LING-FEI HE, SHE-JUAN AN, ZHI-GANG WU, KE-DI YANG, XUE-MIN CHEN. Inhibitory Effects of Selenium on Telomerase Activity and hTERT Expression in Cadmium-transformed 16HBE Cells . Biomedical and Environmental Sciences, 2007, 20(4): 307-312.
[15]	QUN-FENG YAO, XIN-JIANG KANG, QIAO-LING HAO, Yi-Kai Zhou. A Quantitative DNA Methylation Assay Using Mismatch Hybridization and Chemiluminescence . Biomedical and Environmental Sciences, 2005, 18(1): 48-52.
[16]	SHE-JUAN AN, JIA-KUN CHEN, LI-LI LIU, YAN-FENG ZHAO, XUE-MIN CHEN. Over-expressed Genes Detected by Suppression Subtractive Hybridization in Carcinoma Derived From Transformed 16HBE Cells Induced by BPDE . Biomedical and Environmental Sciences, 2005, 18(5): 302-306.
[17]	XUE-JUN YIN, JIAN-NING XU, CHANG-QI ZOU, FENG-SHENG HE, FU-DE FANG. Genes Differentially Expressed in Human Lung Fibroblast Cells Transformed by Glycidyl Methacrylate . Biomedical and Environmental Sciences, 2004, 17(4): 432-441.
[18]	. SUBJECT INDEX FOR VOLUME 16 . Biomedical and Environmental Sciences, 2003, 16(4): 408-414.
[19]	. AUTHOR INDEX FOR VOLUME 16 . Biomedical and Environmental Sciences, 2003, 16(4): 405-407.
[20]	XUE-JUN YIN, FU-DE FANG, JIAN-NING XU, CHANG-QI ZOU, FENG-SHENG HE. Genotoxic and Nongenotoxic Effects of Glycidyl Methacrylate on Human Lung Fibroblast Cells . Biomedical and Environmental Sciences, 2003, 16(3): 283-294.

点击查看大图

计量

文章访问数: 1268
HTML全文浏览量: 387
PDF下载量: 26
被引次数: 0

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

doi: 10.3967/bes2014.002

基金项目: National Natural Science Foundation of China(81072318)

刊出日期: 2014-07-20

关键词:

DNA methylation /

Chip /

Glycidyl methacrylate /

16HBE

摘要: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.

English Abstract

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

National Institute of 0ccupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Funds: National Natural Science Foundation of China(81072318)

Publish Date: 2014-07-20

Keywords:

Abstract: ObjectiveTo establish the model of human bronchial epithelial cells(16HBE) malignant transformation induced by glycidyl methacrylate(GMA)and define the different methylation genes at different stages. MethodsDNA was extracted at different 16HBE malignant phasesandchanges of genes DNA methylation atdifferent stages weredetectedusing Methylation chip of‘NimbleGen HG18 CpG Promoter Microarray Methylation’. Methylation-specific PCR (MSP) was usedto observe the methylation status ofsome genes, and then compared with the control groups. ResultsThe resultshowed that GMA induced 16HBE morphorlogical transformation at the dose of 8μg/mL, and cell exposed to GMA had 1374 genes in protophase, 825 genes inmetaphase, 1149 genes in anaphase, respectively; 30 genes are all methylation in the 3 stages; 318 genes in protophase but not inmetaphase and anaphase; 272 genes in metaphase but not inprotophase and anaphase; 683 genes in anaphase but not inmetaphase and protophase; 73 genes inprotophase andmetaphase but not in anaphase; 67 genes in protophase and anaphase but not inmetaphase; 59 genes inmetaphase and anaphase but not in protophase. ConclusionThe pattern of DNA methylation could change in the process of 16HBEinduced by GMA.

Citation:

WANG An Na, WANG Quan Kai, YANG Min, HU Jie, DONG Lin, andXU Jian Ning. Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation[J]. Biomedical and Environmental Sciences, 2014, 27(7): 523-530. doi: 10.3967/bes2014.002

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/bes2014.002

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

计量

出版历程

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

doi: 10.3967/bes2014.002

English Abstract

Experimental Study onMalignant Transformation of Human Bronchial Epithelial CellsInduced by Glycidyl Methacrylate and Analysis on its Methylation

National Institute of 0ccupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China

全文HTML

目录