PANG Yu; DU Jian; ZHANG Zhi Ying; OU Xi Chao; LI Qiang; XIA Hui; QU Yan

doi:10.3967/bes2014.137

The Feasibility of Sputum Transportation System in China:Effect of Sputum Storage on the Mycobacterial Detection

PANG Yu, DU Jian, ZHANG Zhi Ying, OU Xi Chao, LI Qiang, XIA Hui, QU Yan

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(12): 982-986

PANG Yu, DU Jian, ZHANG Zhi Ying, OU Xi Chao, LI Qiang, XIA Hui, QU Yan. The Feasibility of Sputum Transportation System in China:Effect of Sputum Storage on the Mycobacterial Detection[J]. Biomedical and Environmental Sciences, 2014, 27(12): 982-986. doi: 10.3967/bes2014.137

Citation:

PANG Yu, DU Jian, ZHANG Zhi Ying, OU Xi Chao, LI Qiang, XIA Hui, QU Yan. The Feasibility of Sputum Transportation System in China:Effect of Sputum Storage on the Mycobacterial Detection[J]. Biomedical and Environmental Sciences, 2014, 27(12): 982-986. doi: 10.3967/bes2014.137

doi: 10.3967/bes2014.137

基金项目: This study was supported by Bill and Melinda Gabes Foundation Project (51914)

The Feasibility of Sputum Transportation System in China:Effect of Sputum Storage on the Mycobacterial Detection

National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China
China Office, Program for Appropriate Technology in Health, Beijing 100600, China

Funds: This study was supported by Bill and Melinda Gabes Foundation Project (51914)

摘要: Sputum transportation from county-level to prefecture-level is an ideal strategy to cover the shortage of the laboratory capability in the resource-poor setting.Here,we firstly evaluated the feasibility of sputum transportation system in China by analyzing the culture and molecular diagnosis results from 1982 smear-positive patients with different delay in processing for culture.In this study,the total contamination rate was 2.32％ and the total smear positive/culture negative (S+/C-) rate was 7.57％.We found that sputum specimens refrigerated for no more than 7 d before mycobacterial detection did not affect culture significantly.In addition,the invalid result rates among 0-3 d,3-7 d,and 7+ d group were 3.63％,3.14％,and 12.48％,respectively.Statistic analysis revealed that molecular diagnostic results while the invalid result rate of genechip for the specimen with more than 7 d delay was significantly higher (P＜0.001).The refrigerators equipped in county laboratories,transport at low temperature and frequent transport services once a week will ensure the feasibility of sputum transportation system in China.
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- molecular diagnosis /
- transport services /
- low temperature

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The Feasibility of Sputum Transportation System in China:Effect of Sputum Storage on the Mycobacterial Detection

doi: 10.3967/bes2014.137

基金项目: This study was supported by Bill and Melinda Gabes Foundation Project (51914)

刊出日期: 2014-12-20

关键词:

molecular diagnosis /

transport services /

low temperature

摘要: Sputum transportation from county-level to prefecture-level is an ideal strategy to cover the shortage of the laboratory capability in the resource-poor setting.Here,we firstly evaluated the feasibility of sputum transportation system in China by analyzing the culture and molecular diagnosis results from 1982 smear-positive patients with different delay in processing for culture.In this study,the total contamination rate was 2.32％ and the total smear positive/culture negative (S+/C-) rate was 7.57％.We found that sputum specimens refrigerated for no more than 7 d before mycobacterial detection did not affect culture significantly.In addition,the invalid result rates among 0-3 d,3-7 d,and 7+ d group were 3.63％,3.14％,and 12.48％,respectively.Statistic analysis revealed that molecular diagnostic results while the invalid result rate of genechip for the specimen with more than 7 d delay was significantly higher (P＜0.001).The refrigerators equipped in county laboratories,transport at low temperature and frequent transport services once a week will ensure the feasibility of sputum transportation system in China.

English Abstract

Citation:

PANG Yu, DU Jian, ZHANG Zhi Ying, OU Xi Chao, LI Qiang, XIA Hui, QU Yan. The Feasibility of Sputum Transportation System in China:Effect of Sputum Storage on the Mycobacterial Detection[J]. Biomedical and Environmental Sciences, 2014, 27(12): 982-986. doi: 10.3967/bes2014.137