Volume 25 Issue 1
Nov.  2019
Turn off MathJax
Article Contents

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011
Citation: ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011

Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

doi: 10.3967/0895-3988.2012.01.011
Funds:  This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)
  • Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1~3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100%,100%,and 85%,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(840) PDF downloads(55) Cited by()

Proportional views
Related

Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

doi: 10.3967/0895-3988.2012.01.011
Funds:  This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)

Abstract: Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1~3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100%,100%,and 85%,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011
Citation: ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return