Volume 25 Issue 2
Nov.  2019
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CHEN Hao, ZHENG Zhou, CHEN Peng, WU Xiang Gen, ZHAO Ge. Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 210-215. doi: 10.3967/0895-3988.2012.02.013
Citation: CHEN Hao, ZHENG Zhou, CHEN Peng, WU Xiang Gen, ZHAO Ge. Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 210-215. doi: 10.3967/0895-3988.2012.02.013

Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro

doi: 10.3967/0895-3988.2012.02.013
Funds:  the National Natural Scientific Foundation(81000369)%the Doctor Foundation of Shandong Province(BS2009SW018)
  • Objective Fungal keratitis (FK) is a vision-threatening infection,whose treatment requires more effective and safer anti-fungal agent exploitation urgently.With this aim,we focused on the effect of an extracellular polysaccharide on fungal adhesion to human corneal epithelial cells.Methods We performed the cytotoxicity assays of the extracellular polysaccharide EPS-Ⅱ from an antarctic bacterium Pseudoaltermonas and evaluated its inhibitory effect on Candida albicans cells'adherence to human corneal epithelial cells (HCECs).Results EPS-Ⅱ,which displayed minor cytotoxicity but also promoted proliferation of HCECs,could inhibit the adherence of yeast cells to HCECs in a dose-dependent manner.EPS-Ⅱ could also suppress the subsequent P13K/AKT signaling pathway,and thereby decrease the expression of early inflammatory cytokines.Conclusions Extracellular polysaccharide EPS-Ⅱ was suggested as a new natural agent for attenuating FK.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro

doi: 10.3967/0895-3988.2012.02.013
Funds:  the National Natural Scientific Foundation(81000369)%the Doctor Foundation of Shandong Province(BS2009SW018)

Abstract: Objective Fungal keratitis (FK) is a vision-threatening infection,whose treatment requires more effective and safer anti-fungal agent exploitation urgently.With this aim,we focused on the effect of an extracellular polysaccharide on fungal adhesion to human corneal epithelial cells.Methods We performed the cytotoxicity assays of the extracellular polysaccharide EPS-Ⅱ from an antarctic bacterium Pseudoaltermonas and evaluated its inhibitory effect on Candida albicans cells'adherence to human corneal epithelial cells (HCECs).Results EPS-Ⅱ,which displayed minor cytotoxicity but also promoted proliferation of HCECs,could inhibit the adherence of yeast cells to HCECs in a dose-dependent manner.EPS-Ⅱ could also suppress the subsequent P13K/AKT signaling pathway,and thereby decrease the expression of early inflammatory cytokines.Conclusions Extracellular polysaccharide EPS-Ⅱ was suggested as a new natural agent for attenuating FK.

CHEN Hao, ZHENG Zhou, CHEN Peng, WU Xiang Gen, ZHAO Ge. Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 210-215. doi: 10.3967/0895-3988.2012.02.013
Citation: CHEN Hao, ZHENG Zhou, CHEN Peng, WU Xiang Gen, ZHAO Ge. Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 210-215. doi: 10.3967/0895-3988.2012.02.013

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