Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro
- Extracellular polysaccharide EPS-Ⅱ /
- Candida albicans /
- Cornea /
- Adhesion /
- Inhibitory effect
Abstract: Objective Fungal keratitis (FK) is a vision-threatening infection,whose treatment requires more effective and safer anti-fungal agent exploitation urgently.With this aim,we focused on the effect of an extracellular polysaccharide on fungal adhesion to human corneal epithelial cells.Methods We performed the cytotoxicity assays of the extracellular polysaccharide EPS-Ⅱ from an antarctic bacterium Pseudoaltermonas and evaluated its inhibitory effect on Candida albicans cells'adherence to human corneal epithelial cells (HCECs).Results EPS-Ⅱ,which displayed minor cytotoxicity but also promoted proliferation of HCECs,could inhibit the adherence of yeast cells to HCECs in a dose-dependent manner.EPS-Ⅱ could also suppress the subsequent P13K/AKT signaling pathway,and thereby decrease the expression of early inflammatory cytokines.Conclusions Extracellular polysaccharide EPS-Ⅱ was suggested as a new natural agent for attenuating FK.
|Citation:||CHEN Hao, ZHENG Zhou, CHEN Peng, WU Xiang Gen, ZHAO Ge. Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro[J]. Biomedical and Environmental Sciences, 2012, 25(2): 210-215. doi: 10.3967/0895-3988.2012.02.013|