Volume 28 Issue 4
Nov.  2019
Turn off MathJax
Article Contents

LI Shu Gang, DING Yu Song, NIU Qiang, XU Shang Zhi, PANG Li Juan, MA Ru Lin, JING Ming Xia, FENG Gang Ling, LIU Jia Ming, GUO Shu Xia. Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice[J]. Biomedical and Environmental Sciences, 2015, 28(4): 272-280. doi: 10.3967/bes2015.038
Citation: LI Shu Gang, DING Yu Song, NIU Qiang, XU Shang Zhi, PANG Li Juan, MA Ru Lin, JING Ming Xia, FENG Gang Ling, LIU Jia Ming, GUO Shu Xia. Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice[J]. Biomedical and Environmental Sciences, 2015, 28(4): 272-280. doi: 10.3967/bes2015.038

Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice

doi: 10.3967/bes2015.038
Funds:  a grant from the Xinjiang Production and Construction Corps(2014BA039)%Shihezi University grant(RCZX201112)
  • Objective To determine the ability of grape seed proanthocyanidin extract (GSPE) in alleviating arsenic-induced reproductive toxicity.
    Methods Sixty male Kunming mice received the following treatments by gavage: normal saline solution (control); arsenic trioxide (ATO; 4 mg/kg); GSPE (400 mg/kg); ATO+GSPE (100 mg/kg);ATO+GSPE (200 mg/kg) and ATO+GSPE (400 mg/kg). Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), heme oxygenase 1 (HO1), glutathione S-transferase (GST), NAD(P)H dehydrogenase, and quinone 1 (NQO1) expression in the testis was detected by real-time PCR. Superoxide dismutase (SOD), glutathione (GSH), total antioxidative capability (T-AOC), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and reproductive indexes were analyzed.
    Results ATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO+GSPE group showed recovery of the measured parameters. Mice treated with ATO+high-dose GSPE showed the highest level of mRNA expression of Nrf2, HO, NQO1, and GST.
    Conclusion GSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(997) PDF downloads(30) Cited by()

Proportional views
Related

Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice

doi: 10.3967/bes2015.038
Funds:  a grant from the Xinjiang Production and Construction Corps(2014BA039)%Shihezi University grant(RCZX201112)

Abstract: Objective To determine the ability of grape seed proanthocyanidin extract (GSPE) in alleviating arsenic-induced reproductive toxicity.
Methods Sixty male Kunming mice received the following treatments by gavage: normal saline solution (control); arsenic trioxide (ATO; 4 mg/kg); GSPE (400 mg/kg); ATO+GSPE (100 mg/kg);ATO+GSPE (200 mg/kg) and ATO+GSPE (400 mg/kg). Thereafter, the mice were sacrificed and weighed, and the testis was examined for pathological changes. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), heme oxygenase 1 (HO1), glutathione S-transferase (GST), NAD(P)H dehydrogenase, and quinone 1 (NQO1) expression in the testis was detected by real-time PCR. Superoxide dismutase (SOD), glutathione (GSH), total antioxidative capability (T-AOC), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), and reproductive indexes were analyzed.
Results ATO-treated mice showed a significantly decreased sperm count and testis somatic index and activity levels of SOD, GSH, and T-AOC than control group. Compared to the ATO-treated group, ATO+GSPE group showed recovery of the measured parameters. Mice treated with ATO+high-dose GSPE showed the highest level of mRNA expression of Nrf2, HO, NQO1, and GST.
Conclusion GSPE alleviates oxidative stress damage in mouse testis by activating Nrf2 signaling, thus counteracting arsenic-induced reproductive toxicity.

LI Shu Gang, DING Yu Song, NIU Qiang, XU Shang Zhi, PANG Li Juan, MA Ru Lin, JING Ming Xia, FENG Gang Ling, LIU Jia Ming, GUO Shu Xia. Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice[J]. Biomedical and Environmental Sciences, 2015, 28(4): 272-280. doi: 10.3967/bes2015.038
Citation: LI Shu Gang, DING Yu Song, NIU Qiang, XU Shang Zhi, PANG Li Juan, MA Ru Lin, JING Ming Xia, FENG Gang Ling, LIU Jia Ming, GUO Shu Xia. Grape Seed Proanthocyanidin Extract Alleviates Arsenic-induced Oxidative Reproductive Toxicity in Male Mice[J]. Biomedical and Environmental Sciences, 2015, 28(4): 272-280. doi: 10.3967/bes2015.038

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return