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Blood was collected from October 27 to November 2, 2017 at Shidatou village in Dejiang prefecture in Guizhou Province. This study analyzed the samples from 366 healthy individuals collected from eight groups in Shidatou (the Yujiashan, Youjia, Shidatou, Pachuanba, Caijiaxi, Jinjiagou, Jiangjiatuo, and Xiaping groups) and 104 domestic animals (63 chickens, 11 pigs, and 30 sheep) from the Yujiashan, Shidatou, and Jiangjiatuo groups (Figure 1, Table 1). Of the human subjects, 29.9% were 0–19 years old, 28.1% were 20–49 years old, and 42.0% were ≥ 50 years old.
Variable Number (n) Constituent ratio (n%) Human Age group (Year) 0−9 69 18.8 10−19 40 10.9 20−29 26 7.1 30−39 31 8.5 40−49 46 12.5 50−59 54 14.7 60−69 63 17.2 > 70 37 10.1 Sex Man 171 46.7 Woman 195 53.2 Nationality Tu 364 99.4 Han 2 0.6 Total 366 Animal Chicken 63 60.5 pig 11 10.5 sheep 30 28.8 Total 104 Table 1. Summary of the collected serum of human health and domestic animal
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None of the human serum specimens tested positive for ZIKV PRNT90, while three animals (chicken D32, sheep D56, and sheep D61) tested positive, with neutralizing antibody titers of 1:10, 1:20, and 1:40, respectively (Table 2). The three positive animal specimens were further tested for JEV neutralizing antibody titers: D32 was negative, while D56 and D61 were positive for JEV PRNT90 neutralizing antibody, with titers of 1:40 and 1:80, respectively (Table 2). As the titers of the two viruses did not reach a 4-fold difference in the same specimen, D56 and D61 were identified as being coinfected with ZIKV and JEV.
Sample ID Collection site Host species PRNT90 titer* ZIKV JEV D32 Yujiashan chicken 1:10 − D56 Shidatou sheep 1:20 1:40 D61 Shidatou sheep 1:40 1:80 Note.*PRNT 90 titer, 90% plaque reduction neutralization test titer. Table 2. Neutralizing antibody titer to ZIKA and JEV infection among domestic animals in Shidatou village
In summary, all 366 residents and 11 pigs whose samples were collected, tested negative for ZIKV neutralizing antibody; 1 of 63 chickens (1.59%) was positive for ZIKV neutralizing antibody but negative for JEV, and 2 of 30 sheep (6.67%) were positive for both ZIKV and JEV neutralizing antibodies (Table 3).
Host species No. case No. PRNT90 positive Seroprevalence (%) ZIKV JEV Human 366 − − − Chicken 63 1 − 1.59 Pig 11 − − − Sheep 30 2 2 6.67 Table 3. The seroprevalence of ZIKV in human and domestic animals in Shidatou village
Serological Survey of Zika Virus in Humans and Animals in Dejiang Prefecture, Guizhou Province, China
doi: 10.3967/bes2019.108
- Received Date: 2019-09-09
- Accepted Date: 2019-11-11
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Key words:
- Zika Virus /
- Serological survey /
- Humans /
- Animals /
- China /
- Plague reduction neutralization test
Abstract:
Citation: | LI Fan, ZHOU Jing Zhu, ZHOU Lei, FU Shi Hong, TIAN Zhen Zao, WANG Qi, SHAO Nan, LI Dan, HE Ying, LEI Wen Wen, TANG Guang Peng, LIANG Guo Dong, WANG Ding Ming, ZHANG Yan Ping, WANG Huan Yu. Serological Survey of Zika Virus in Humans and Animals in Dejiang Prefecture, Guizhou Province, China[J]. Biomedical and Environmental Sciences, 2019, 32(12): 875-880. doi: 10.3967/bes2019.108 |