Volume 15 Issue 3
Nov.  2019
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BHOLA NATH PAUL, ANAND PRAKASH, SIRISH KUMAR, AJAY K.YADAV, U.MANI, ASHOK K.SAXENA, ANAND PRAKASH SAHU, KEWAL LAL, KALYAN K.DUTTA. Silica Induced Early Fibrogenic Reaction in Lung of Mice Ameliorated by Nyctanthes arbortristis Extract[J]. Biomedical and Environmental Sciences, 2002, 15(3): 215-222.
Citation: BHOLA NATH PAUL, ANAND PRAKASH, SIRISH KUMAR, AJAY K.YADAV, U.MANI, ASHOK K.SAXENA, ANAND PRAKASH SAHU, KEWAL LAL, KALYAN K.DUTTA. Silica Induced Early Fibrogenic Reaction in Lung of Mice Ameliorated by Nyctanthes arbortristis Extract[J]. Biomedical and Environmental Sciences, 2002, 15(3): 215-222.

Silica Induced Early Fibrogenic Reaction in Lung of Mice Ameliorated by Nyctanthes arbortristis Extract

Funds:  Foundation of the Department of Biotechnology, New Delhi(grant BT/Pro892/PB/17/042/97)
  • Objective To investigate the pharmacological effect of Nyctanthes arbortristis (NAT) leaf extract in the prevention of lung injury induced by silica particles. Method Lung injury was induced in Swiss mice through inhalation exposure to silica particles (<5μ) using a Flow Past Nose Only Inhalation Chamber at the rate of -10 mg/m3 respirable mass for 5 h. Lung bronchoalveolar lavage (BAL) fluid collected between 48 and 72 h was subjected to protein profiling by electrophoresis and cytokine evaluation by solid phase sandwich ELISA. Lung histopathology was performed to evaluate lung injury. Results Inhalation of silica increased the level of tumor necrosis factor-α(TNF-α), and of the 66 and 63 kDa peptides in the BAL fluid in comparison to sham-treated control. Pre-treatment of silica exposed mice with NAT leaf extract significantly prevented the accumulation of TNF-α in the BAL fluid, but the 66 and 63 kDa peptides remained unchanged. The extract was also effective in the prevention of silica-induced early fibrogenic reactions like congestion, edema and infiltration of nucleated cells in the interstitial alveolar spaces, and thickening of alveolar septa in mouse lung. Conclusion NAT leaf extract helps in bypassing silica induced initial lung injury in mice.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Silica Induced Early Fibrogenic Reaction in Lung of Mice Ameliorated by Nyctanthes arbortristis Extract

Funds:  Foundation of the Department of Biotechnology, New Delhi(grant BT/Pro892/PB/17/042/97)

Abstract: Objective To investigate the pharmacological effect of Nyctanthes arbortristis (NAT) leaf extract in the prevention of lung injury induced by silica particles. Method Lung injury was induced in Swiss mice through inhalation exposure to silica particles (<5μ) using a Flow Past Nose Only Inhalation Chamber at the rate of -10 mg/m3 respirable mass for 5 h. Lung bronchoalveolar lavage (BAL) fluid collected between 48 and 72 h was subjected to protein profiling by electrophoresis and cytokine evaluation by solid phase sandwich ELISA. Lung histopathology was performed to evaluate lung injury. Results Inhalation of silica increased the level of tumor necrosis factor-α(TNF-α), and of the 66 and 63 kDa peptides in the BAL fluid in comparison to sham-treated control. Pre-treatment of silica exposed mice with NAT leaf extract significantly prevented the accumulation of TNF-α in the BAL fluid, but the 66 and 63 kDa peptides remained unchanged. The extract was also effective in the prevention of silica-induced early fibrogenic reactions like congestion, edema and infiltration of nucleated cells in the interstitial alveolar spaces, and thickening of alveolar septa in mouse lung. Conclusion NAT leaf extract helps in bypassing silica induced initial lung injury in mice.

BHOLA NATH PAUL, ANAND PRAKASH, SIRISH KUMAR, AJAY K.YADAV, U.MANI, ASHOK K.SAXENA, ANAND PRAKASH SAHU, KEWAL LAL, KALYAN K.DUTTA. Silica Induced Early Fibrogenic Reaction in Lung of Mice Ameliorated by Nyctanthes arbortristis Extract[J]. Biomedical and Environmental Sciences, 2002, 15(3): 215-222.
Citation: BHOLA NATH PAUL, ANAND PRAKASH, SIRISH KUMAR, AJAY K.YADAV, U.MANI, ASHOK K.SAXENA, ANAND PRAKASH SAHU, KEWAL LAL, KALYAN K.DUTTA. Silica Induced Early Fibrogenic Reaction in Lung of Mice Ameliorated by Nyctanthes arbortristis Extract[J]. Biomedical and Environmental Sciences, 2002, 15(3): 215-222.

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