Volume 16 Issue 1
Nov.  2019
Turn off MathJax
Article Contents

YI-HUA AN, Hong WAN, Ze-Shun ZHANG, Hong-Yun WANG, ZHI-XING GAO, Mei-Zhen SUN, Zhong-Cheng WANG. Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells[J]. Biomedical and Environmental Sciences, 2003, 16(1): 90-94.
Citation: YI-HUA AN, Hong WAN, Ze-Shun ZHANG, Hong-Yun WANG, ZHI-XING GAO, Mei-Zhen SUN, Zhong-Cheng WANG. Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells[J]. Biomedical and Environmental Sciences, 2003, 16(1): 90-94.

Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells

Funds:  This research is supported by grants from the Chinese Postdoctoral Foundation and the Beijing Young Scientist Culture Foundation
  • Objective To investigate the effect of rat Schwann cell secretion on the proliferation and differentiation of human embryonic neural stem cells (NSCs). Methods The samples were divided into three groups. In Group One, NSCs were cultured in DMED/F12 in which Schwann cells had grown for one day. In Group Two, NSCs and Schwann cells were co-cultured. In Group Three, NSCs were cultured in DMEM/F12. The morphology of NSCs was checked and b-tubulin, GalC, hoechst 33342 and GFAP labellings were detected. Results In Group One, all neural spheres were attached to the bottom and differentiated. The majority of them were b-tubulin positive while a few of cells were GFAP or GalC positive. In Group Two, neural spheres remained undifferentiatied and their proliferation was inhibited in places where Schwann cells were robust. In places where there were few Schwann cells, NSCs performed in a similar manner as in Group One. In Group Three, the cell growth state deteriorated day after day. On the 7th day, most NSCs died. Conclusion The secretion of rat Schwann cells has a growth supportive and differentiation-inducing effect on human NSCs.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(611) PDF downloads(16) Cited by()

Proportional views
Related

Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells

Funds:  This research is supported by grants from the Chinese Postdoctoral Foundation and the Beijing Young Scientist Culture Foundation

Abstract: Objective To investigate the effect of rat Schwann cell secretion on the proliferation and differentiation of human embryonic neural stem cells (NSCs). Methods The samples were divided into three groups. In Group One, NSCs were cultured in DMED/F12 in which Schwann cells had grown for one day. In Group Two, NSCs and Schwann cells were co-cultured. In Group Three, NSCs were cultured in DMEM/F12. The morphology of NSCs was checked and b-tubulin, GalC, hoechst 33342 and GFAP labellings were detected. Results In Group One, all neural spheres were attached to the bottom and differentiated. The majority of them were b-tubulin positive while a few of cells were GFAP or GalC positive. In Group Two, neural spheres remained undifferentiatied and their proliferation was inhibited in places where Schwann cells were robust. In places where there were few Schwann cells, NSCs performed in a similar manner as in Group One. In Group Three, the cell growth state deteriorated day after day. On the 7th day, most NSCs died. Conclusion The secretion of rat Schwann cells has a growth supportive and differentiation-inducing effect on human NSCs.

YI-HUA AN, Hong WAN, Ze-Shun ZHANG, Hong-Yun WANG, ZHI-XING GAO, Mei-Zhen SUN, Zhong-Cheng WANG. Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells[J]. Biomedical and Environmental Sciences, 2003, 16(1): 90-94.
Citation: YI-HUA AN, Hong WAN, Ze-Shun ZHANG, Hong-Yun WANG, ZHI-XING GAO, Mei-Zhen SUN, Zhong-Cheng WANG. Effect of Rat Schwann Cell Secretion on Proliferation and Differentiation of Human Neural Stem Cells[J]. Biomedical and Environmental Sciences, 2003, 16(1): 90-94.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return