Volume 16 Issue 1
Nov.  2019
Turn off MathJax
Article Contents

K.Krishnamurthi, Font Devi, T.Chakrabarti. Genotoxic Effects of PAH Containing Sludge Extracts in Chinese Hamster Ovary Cell Cultures[J]. Biomedical and Environmental Sciences, 2003, 16(1): 68-82.
Citation: K.Krishnamurthi, Font Devi, T.Chakrabarti. Genotoxic Effects of PAH Containing Sludge Extracts in Chinese Hamster Ovary Cell Cultures[J]. Biomedical and Environmental Sciences, 2003, 16(1): 68-82.

Genotoxic Effects of PAH Containing Sludge Extracts in Chinese Hamster Ovary Cell Cultures

  • Objective Many studies have been conducted in order to evaluate the genotoxicity of chemicals and waste materials, which utilized in vivo test protocols. The use of animals for routine toxicity testing is now questioned by a growing segment of society[1]. Methods Keeping the above fact in mind, we have conducted in the present study the genotoxicity evaluation of oily sludge samples generated from a petroleum refinery and petrochemical industry and ETP sludge from petroleum refinery using DNA damage, chromosomal aberration, p53 protein induction and apoptosis in short term in vitro mammalian Chinese Hamster Ovary cell cultures. Results It is evident from the results that the oily sludge compounds derived from petroleum refinery and petrochemical industry could cause DNA damage, chromosomal aberration, p53 protein accumulation and apoptotic cell death on exposure to oily sludge extracts in the presence of metabolic activation system (S-9 mix), however, ETP sludge extract could not cause significant genotoxicity in comparison to oily sludge extract and negative control. Conclusion The effect may be attributed to polycyclic aromatic hydrocarbons present in the samples as evidenced from GC-MS.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(650) PDF downloads(11) Cited by()

Proportional views
Related

Genotoxic Effects of PAH Containing Sludge Extracts in Chinese Hamster Ovary Cell Cultures

Abstract: Objective Many studies have been conducted in order to evaluate the genotoxicity of chemicals and waste materials, which utilized in vivo test protocols. The use of animals for routine toxicity testing is now questioned by a growing segment of society[1]. Methods Keeping the above fact in mind, we have conducted in the present study the genotoxicity evaluation of oily sludge samples generated from a petroleum refinery and petrochemical industry and ETP sludge from petroleum refinery using DNA damage, chromosomal aberration, p53 protein induction and apoptosis in short term in vitro mammalian Chinese Hamster Ovary cell cultures. Results It is evident from the results that the oily sludge compounds derived from petroleum refinery and petrochemical industry could cause DNA damage, chromosomal aberration, p53 protein accumulation and apoptotic cell death on exposure to oily sludge extracts in the presence of metabolic activation system (S-9 mix), however, ETP sludge extract could not cause significant genotoxicity in comparison to oily sludge extract and negative control. Conclusion The effect may be attributed to polycyclic aromatic hydrocarbons present in the samples as evidenced from GC-MS.

K.Krishnamurthi, Font Devi, T.Chakrabarti. Genotoxic Effects of PAH Containing Sludge Extracts in Chinese Hamster Ovary Cell Cultures[J]. Biomedical and Environmental Sciences, 2003, 16(1): 68-82.
Citation: K.Krishnamurthi, Font Devi, T.Chakrabarti. Genotoxic Effects of PAH Containing Sludge Extracts in Chinese Hamster Ovary Cell Cultures[J]. Biomedical and Environmental Sciences, 2003, 16(1): 68-82.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return