Volume 17 Issue 4
Nov.  2019
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XUE-JUN YIN, JIAN-NING XU, CHANG-QI ZOU, FENG-SHENG HE, FU-DE FANG. Genes Differentially Expressed in Human Lung Fibroblast Cells Transformed by Glycidyl Methacrylate[J]. Biomedical and Environmental Sciences, 2004, 17(4): 432-441.
Citation: XUE-JUN YIN, JIAN-NING XU, CHANG-QI ZOU, FENG-SHENG HE, FU-DE FANG. Genes Differentially Expressed in Human Lung Fibroblast Cells Transformed by Glycidyl Methacrylate[J]. Biomedical and Environmental Sciences, 2004, 17(4): 432-441.

Genes Differentially Expressed in Human Lung Fibroblast Cells Transformed by Glycidyl Methacrylate

Funds:  国家自然科学基金(grant 39840017)
  • To define the differences in gene expression patterns between glycidyl methacrylate (GMA)-transformed human lung fibroblast cells (2BS cells) and controls. Methods The mRNA differential display polymerase chain reaction (DD-PCR) technique was used. cDNAs were synthesized by reverse transcription and amplified by PCR using 30 primer combinations. After being screened by dot blot analysis, differentially expressed cDNAs were cloned, sequenced and confirmed by Northern blot analysis. Results Eighteen differentially expressed cDNAs were cloned and sequenced, of which 17 were highly homologous to known genes (homology = 89%-100%) and one was an unknown gene. Northern blot analysis confirmed that eight genes encoding human zinc finger protein 217 (ZNF217), mixed-lineage kinase 3 (MLK-3), ribosomal protein (RP) L15, RPL41, RPS16, TBX3, stanniocalcin 2 (STC2) and mouse ubiquitin conjugating enzyme (UBC), respectively, were up-regulated, and three genes including human transforming growth factor ( inducible gene (Betaig-h3), (-1,2-mannosidase 1A2 (MAN 1A2) gene and an unknown gene were down-regulated in the GMA-transformed cells. Conclusion Analysis of the potential function of these genes suggest that they may be possibly linked to a variety of cellular processes such as transcription, signal transduction, protein synthesis and growth, and that their differential expression could contribute to the GMA-induced neoplastic transformation.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Genes Differentially Expressed in Human Lung Fibroblast Cells Transformed by Glycidyl Methacrylate

Funds:  国家自然科学基金(grant 39840017)

Abstract: To define the differences in gene expression patterns between glycidyl methacrylate (GMA)-transformed human lung fibroblast cells (2BS cells) and controls. Methods The mRNA differential display polymerase chain reaction (DD-PCR) technique was used. cDNAs were synthesized by reverse transcription and amplified by PCR using 30 primer combinations. After being screened by dot blot analysis, differentially expressed cDNAs were cloned, sequenced and confirmed by Northern blot analysis. Results Eighteen differentially expressed cDNAs were cloned and sequenced, of which 17 were highly homologous to known genes (homology = 89%-100%) and one was an unknown gene. Northern blot analysis confirmed that eight genes encoding human zinc finger protein 217 (ZNF217), mixed-lineage kinase 3 (MLK-3), ribosomal protein (RP) L15, RPL41, RPS16, TBX3, stanniocalcin 2 (STC2) and mouse ubiquitin conjugating enzyme (UBC), respectively, were up-regulated, and three genes including human transforming growth factor ( inducible gene (Betaig-h3), (-1,2-mannosidase 1A2 (MAN 1A2) gene and an unknown gene were down-regulated in the GMA-transformed cells. Conclusion Analysis of the potential function of these genes suggest that they may be possibly linked to a variety of cellular processes such as transcription, signal transduction, protein synthesis and growth, and that their differential expression could contribute to the GMA-induced neoplastic transformation.

XUE-JUN YIN, JIAN-NING XU, CHANG-QI ZOU, FENG-SHENG HE, FU-DE FANG. Genes Differentially Expressed in Human Lung Fibroblast Cells Transformed by Glycidyl Methacrylate[J]. Biomedical and Environmental Sciences, 2004, 17(4): 432-441.
Citation: XUE-JUN YIN, JIAN-NING XU, CHANG-QI ZOU, FENG-SHENG HE, FU-DE FANG. Genes Differentially Expressed in Human Lung Fibroblast Cells Transformed by Glycidyl Methacrylate[J]. Biomedical and Environmental Sciences, 2004, 17(4): 432-441.

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