Comparative Proteomic Analysis of B. henselae Houston and B. henselae Marseille by Two-dimensional Gel Electrophoresis

SU-QING ZHAO; YAN-FEI CAI; ZHEN-YU ZHU

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 18 Issue 5

Oct. 2005

Turn off MathJax

Article Contents

Article Navigation > Biomedical and Environmental Sciences > 2005 > 18(5): 341-344

SU-QING ZHAO, YAN-FEI CAI, ZHEN-YU ZHU. Comparative Proteomic Analysis of B. henselae Houston and B. henselae Marseille by Two-dimensional Gel Electrophoresis[J]. Biomedical and Environmental Sciences, 2005, 18(5): 341-344.

Citation:

SU-QING ZHAO, YAN-FEI CAI, ZHEN-YU ZHU. Comparative Proteomic Analysis of B. henselae Houston and B. henselae Marseille by Two-dimensional Gel Electrophoresis[J]. Biomedical and Environmental Sciences, 2005, 18(5): 341-344.

Comparative Proteomic Analysis of B. henselae Houston and B. henselae Marseille by Two-dimensional Gel Electrophoresis

Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France;Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090,Guangdong, China
College of Resource and Environment, South China Agriculture University,Guangzhou 510642, Guangdong, China
Department of Biochemistry, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou 510080, Guangdong, China

Abstract

Objective To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis. Method Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis. Results Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 μg/μL and 2.200 μg/μL respectively. Sample protein of 40 μg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae. Conclusion The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.
- Protein,
- B. henselae,
- Two-dimensional gel electrophoresis
References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views(1063) PDF downloads(15) Cited by()

Proportional views

Comparative Proteomic Analysis of B. henselae Houston and B. henselae Marseille by Two-dimensional Gel Electrophoresis

Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France;Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090,Guangdong, China

College of Resource and Environment, South China Agriculture University,Guangzhou 510642, Guangdong, China

Department of Biochemistry, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou 510080, Guangdong, China

Key words:

Abstract: Objective To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis. Method Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis. Results Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 μg/μL and 2.200 μg/μL respectively. Sample protein of 40 μg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae. Conclusion The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.

Citation: