Volume 22 Issue 3
Jun.  2009
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Article Navigation >  Biomedical and Environmental Sciences  > 2009  >  22(3): 253-258

AI-HUA SUN, XING-LI FAN, LI-WEI LI, LI-FANG WANG, WEN-YING AN, JIE YAN. Rapid Detection of rpoB Gene Mutations in Rif-resistant M.tuberculosis Isolates by Obligonucleotide Microarray[J]. Biomedical and Environmental Sciences, 2009, 22(3): 253-258.
Citation: AI-HUA SUN, XING-LI FAN, LI-WEI LI, LI-FANG WANG, WEN-YING AN, JIE YAN. Rapid Detection of rpoB Gene Mutations in Rif-resistant M.tuberculosis Isolates by Obligonucleotide Microarray[J]. Biomedical and Environmental Sciences, 2009, 22(3): 253-258.
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Rapid Detection of rpoB Gene Mutations in Rif-resistant M.tuberculosis Isolates by Obligonucleotide Microarray

  • Department of Basic Medicine,Zhejiang Medical College,Hangzhou 310053,Zhejiang,China

  • Department of Medical Microbiology and Parasitology,Zhejiang University,Hangzhou310058,Zhejiang,China

  • School of International Study,Zhejiang University,Hangzhou 310058,Zhejiang,China

Funds:  The National Natural Science Foundation of China(30400018)
  • Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray.Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains.Target DNA of M.tuberculosis was amplified by PCR,hybridized and scanned.Direct sequencing was performed to verify the results of oligonuclcotide microarray.Results of the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes. Conclusion Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M.tuberculosis isolates.
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Rapid Detection of rpoB Gene Mutations in Rif-resistant M.tuberculosis Isolates by Obligonucleotide Microarray

  • Department of Basic Medicine,Zhejiang Medical College,Hangzhou 310053,Zhejiang,China
  • Department of Medical Microbiology and Parasitology,Zhejiang University,Hangzhou310058,Zhejiang,China
  • School of International Study,Zhejiang University,Hangzhou 310058,Zhejiang,China
Funds:  The National Natural Science Foundation of China(30400018)

Abstract: Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray.Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains.Target DNA of M.tuberculosis was amplified by PCR,hybridized and scanned.Direct sequencing was performed to verify the results of oligonuclcotide microarray.Results of the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes. Conclusion Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M.tuberculosis isolates.

AI-HUA SUN, XING-LI FAN, LI-WEI LI, LI-FANG WANG, WEN-YING AN, JIE YAN. Rapid Detection of rpoB Gene Mutations in Rif-resistant M.tuberculosis Isolates by Obligonucleotide Microarray[J]. Biomedical and Environmental Sciences, 2009, 22(3): 253-258.
Citation: AI-HUA SUN, XING-LI FAN, LI-WEI LI, LI-FANG WANG, WEN-YING AN, JIE YAN. Rapid Detection of rpoB Gene Mutations in Rif-resistant M.tuberculosis Isolates by Obligonucleotide Microarray[J]. Biomedical and Environmental Sciences, 2009, 22(3): 253-258.
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