Inhibitory Effects of Alpha-zearalenol on Angiotensin II-Induced Integrin β3 mRNA via Suppression of Nuclear Factor-κB

SU-MIN LI; XIAO-MING WANG; JIN QIU; QIN SI; HENG-YI GUO; REN-YU SUN; QI-XIA WU

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Volume 18 Issue 5

Oct. 2005

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Article Navigation > Biomedical and Environmental Sciences > 2005 > 18(5): 314-320

SU-MIN LI, XIAO-MING WANG, JIN QIU, QIN SI, HENG-YI GUO, REN-YU SUN, QI-XIA WU. Inhibitory Effects of Alpha-zearalenol on Angiotensin II-Induced Integrin β3 mRNA via Suppression of Nuclear Factor-κB[J]. Biomedical and Environmental Sciences, 2005, 18(5): 314-320.

Citation:

SU-MIN LI, XIAO-MING WANG, JIN QIU, QIN SI, HENG-YI GUO, REN-YU SUN, QI-XIA WU. Inhibitory Effects of Alpha-zearalenol on Angiotensin II-Induced Integrin β3 mRNA via Suppression of Nuclear Factor-κB[J]. Biomedical and Environmental Sciences, 2005, 18(5): 314-320.

Inhibitory Effects of Alpha-zearalenol on Angiotensin II-Induced Integrin β3 mRNA via Suppression of Nuclear Factor-κB

Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China

Funds: 国家自然科学基金(39730020, 39730220)

Abstract

Objective To investigate the effect of ?-zearalenol on angiotensin II-induced β3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs). Methods The mRNA level in integrin β3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-(B activity was determined by the luciferase activity assay of plasmid NF-(B-LUC. Results The angiotensin II- induced β3 integrin mRNA expression was inhibited by α-zearalenol and 17β-estradiol (10 nmol/L -1 (mol/L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17β-estradiol suppressed the angiotensin II-induced activation of NF-κB in endothelial cells. Conclusion Alpha-zearalenol inhibits angiotensin II-induced integrin β3 mRNA expression by suppressing NF-κB activation in endothelial cells.
- Alpha-zearalenol,
- Integrin β3,
- Endothelial cell,
- NF-κB,
- 17β-estradiol
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Inhibitory Effects of Alpha-zearalenol on Angiotensin II-Induced Integrin β3 mRNA via Suppression of Nuclear Factor-κB

Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China

Funds: 国家自然科学基金(39730020, 39730220)

Key words:

Abstract: Objective To investigate the effect of ?-zearalenol on angiotensin II-induced β3 integrin mRNA expression in human umbilical vein endothelial cells (HUVECs). Methods The mRNA level in integrin β3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-(B activity was determined by the luciferase activity assay of plasmid NF-(B-LUC. Results The angiotensin II- induced β3 integrin mRNA expression was inhibited by α-zearalenol and 17β-estradiol (10 nmol/L -1 (mol/L), but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17β-estradiol suppressed the angiotensin II-induced activation of NF-κB in endothelial cells. Conclusion Alpha-zearalenol inhibits angiotensin II-induced integrin β3 mRNA expression by suppressing NF-κB activation in endothelial cells.

Citation:

SU-MIN LI, XIAO-MING WANG, JIN QIU, QIN SI, HENG-YI GUO, REN-YU SUN, QI-XIA WU. Inhibitory Effects of Alpha-zearalenol on Angiotensin II-Induced Integrin β3 mRNA via Suppression of Nuclear Factor-κB[J]. Biomedical and Environmental Sciences, 2005, 18(5): 314-320.