HU Ying; CHEN ErJun; MU Yue; LI JinLu; CHEN RenJi

doi:10.3967/0895-3988.2011.02.001

BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

HU Ying, CHEN ErJun, MU Yue, LI JinLu, CHEN RenJi

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(2): 89-93

HU Ying, CHEN ErJun, MU Yue, LI JinLu, CHEN RenJi. BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population[J]. Biomedical and Environmental Sciences, 2011, 24(2): 89-93. doi: 10.3967/0895-3988.2011.02.001

Citation:

HU Ying, CHEN ErJun, MU Yue, LI JinLu, CHEN RenJi. BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population[J]. Biomedical and Environmental Sciences, 2011, 24(2): 89-93. doi: 10.3967/0895-3988.2011.02.001

doi: 10.3967/0895-3988.2011.02.001

基金项目: the National Natural Science Foundation of China(307009907)%Beijing Natural Science Foundation(7082038)%Beijing Foundation for Excellent Elite(2010D003034000013)

BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China
Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Funds: the National Natural Science Foundation of China(307009907)%Beijing Natural Science Foundation(7082038)%Beijing Foundation for Excellent Elite(2010D003034000013)

摘要: Objective Convincing evidence suggests a link between increased risk of nonsyndromic cleft lip with or without cleft palate (NSCL/P) and low intake of folic acid by the mother during pregnancy. The present study was designed to explore if genetic variation in the betaine-homocysteine methyltransferase (BHMT) gene contributes to NSCL/P. Methods DNA was obtained from 166 individuals with NSCL/P and 285 healthy subjects. Three known single nucleotide polymorphisms (SNPs) present in the BHMT gene (rs651852, rs3797546, and rs3733890) were investigated by real-time PCR-based TaqMan genotyping. Results Neither allelic nor genotypic association was found between NSCL/P and SNPs rs651852 and rs3733890. SNP rs3797546 did not show allelic association with NSCL/P; however, a higher proportion of NSCL/P patients carry the CC genotype compared with the TT+CT genotype (P=0.020, OR=2.10, 95% Cl=1.11-3.95). Conclusion Our study suggests that polymorphism rs3797546 in the BHMT gene may confer genetic risk of NSCL/P in a recessive manner.
- /
- /
- /
Abstract: Objective Convincing evidence suggests a link between increased risk of nonsyndromic cleft lip with or without cleft palate (NSCL/P) and low intake of folic acid by the mother during pregnancy. The present study was designed to explore if genetic variation in the betaine-homocysteine methyltransferase (BHMT) gene contributes to NSCL/P. Methods DNA was obtained from 166 individuals with NSCL/P and 285 healthy subjects. Three known single nucleotide polymorphisms (SNPs) present in the BHMT gene (rs651852, rs3797546, and rs3733890) were investigated by real-time PCR-based TaqMan genotyping. Results Neither allelic nor genotypic association was found between NSCL/P and SNPs rs651852 and rs3733890. SNP rs3797546 did not show allelic association with NSCL/P; however, a higher proportion of NSCL/P patients carry the CC genotype compared with the TT+CT genotype (P=0.020, OR=2.10, 95% Cl=1.11-3.95). Conclusion Our study suggests that polymorphism rs3797546 in the BHMT gene may confer genetic risk of NSCL/P in a recessive manner.
- Nonsyndromic cleft lip with or without palate /
- Homocysteine /
- Folate /
- Polymorphisms

[1]	Zhenyu Guo, Hongtian Li, Yirui Ma, Ying Meng, Yubo Zhou, Jianmeng Liu. Homocysteine Levels and Determinants among Chinese Women at Mid-pregnancy, Late Pregnancy, and Postpartum . Biomedical and Environmental Sciences, 2024, 37(): 1-11. doi: 10.3967/bes2024.155
[2]	ZHU Qian Rang, KOK E Dieuwertje, BEKELE Hailu Tesfaye, MANUSAMA Koen, ZHANG Jing Xian, XIE Wei, ZONG Wen Qi, ZUO Hui, ZHANG Jian, KAMPMAN Ellen, DAI Yue. Association of Serum Folate and Vitamin B₁₂ Concentrations with Obesity in Chinese Children and Adolescents . Biomedical and Environmental Sciences, 2024, 37(3): 242-253. doi: 10.3967/bes2024.028
[3]	WANG Shan, ZENG Yu Bing, PEI Pei, HE Xue Jia, LIU Fan, WANG Yi, ZHANG Ting. Characterization of CircRNA-Associated CeRNA Networks in Folate Deficiency-Induced Neural Tube Defects . Biomedical and Environmental Sciences, 2023, 36(9): 837-849. doi: 10.3967/bes2023.071
[4]	. Method for Folate Deficiency Screening . Biomedical and Environmental Sciences, 2023, 36(8): 784-784. doi: 10.3967/bes2023.108
[5]	LIU Zhen, MAN Qing Qing, JIA Shan Shan, SONG Peng Kun, WANG Jing Zhong, WANG Xi Le, KANG Bing Xian, YANG Li Gui, ZHANG Jian. Folate and Vitamin B₁₂ Status and Related Dietary Factors among the Elderly Northern and Southern Rural Chinese Population . Biomedical and Environmental Sciences, 2021, 34(7): 552-556. doi: 10.3967/bes2021.075
[6]	LIU Yi Qun, HUANG Ling He, LIU Pei Pei, XING Qing Bin, HAN Feng, WANG Qin, CHEN Shu Rong, SUGIYAMA Kimio, XIANG Xue Song, HUANG Zhen Wu. Effect of N,N-Dimethylglycine on Homocysteine Metabolism in Rats Fed Folate-Sufficient and Folate-Deficient Diets . Biomedical and Environmental Sciences, 2021, 34(5): 356-363. doi: 10.3967/bes2021.047
[7]	WANG Yu Heng, YAN Qing Hua, XU Ji Ying, LI Xin Jian, CHENG Min Na. High Prevalence and Factors Contributing to Hyperhomocysteinemia, Folate Deficiency, and Vitamin B₁₂ Deficiency among Healthy Adults in Shanghai, China . Biomedical and Environmental Sciences, 2019, 32(1): 63-67. doi: 10.3967/bes2019.010
[8]	GAO Li Yun, ZHANG Feng Quan, ZHAO WEI Hui, HAN Guang Liang, WANG Xiao, LI Qiang, GAO Shan Shan, WU Wei Dong. LncRNA H19 and Target Gene-mediated Cleft Palate Induced by TCDD . Biomedical and Environmental Sciences, 2017, 30(9): 676-680. doi: 10.3967/bes2017.090
[9]	ZHANG Qun, LIU Di, ZHAO ZhongYao, SUN Qi, DING LiXiang, WANG YouXin. Association between the SUMO4 M55V Polymorphism and Susceptibility to Type 2 Diabetes Mellitus: A Meta-analysis . Biomedical and Environmental Sciences, 2017, 30(4): 288-295. doi: 10.3967/bes2017.038
[10]	SONG Ying, TANG Xiao Fang, YAO Yi, HE Chen, XU Jing Jing, WANG Huan Huan, GAO Zhan, WANG Miao, YUAN Jin Qing. Association of α_2A-Adrenergic Receptor Genetic Variants with Platelet Reactivity in Chinese Patients on Dual Antiplatelet Therapy Undergoing Percutaneous Coronary Intervention . Biomedical and Environmental Sciences, 2017, 30(12): 898-906. doi: 10.3967/bes2017.120
[11]	ZHOU Jun Mei, ZHANG Ming, WANG Shu, WANG Bing Yuan, HAN Cheng Yi, REN Yong Cheng, ZHANG Lu, ZHANG Hong Yan, YANG Xiang Yu, ZHAO Yang, HU Dong Sheng. Association of the ADIPOQ Rs2241766 and Rs266729 Polymorphisms with Metabolic Syndrome in the Chinese Population：A Meta-analysis . Biomedical and Environmental Sciences, 2016, 29(7): 505-515. doi: 10.3967/bes2016.066
[12]	XU Li Fang, ZHOU Xiao Long, WANG Qi, ZHOU Ji Long, LIU Ya Peng, JU Qiang, WANG Hui, ZHANG Jin Peng, WU Qing Rong, LI Yi Qun, XIA Yu Juan, PENG Xiu, ZHANG Mei Rong, YU Hong Min, XU Li Chun. A Case-control Study of Environmental Risk Factors for Nonsyndromic Cleft of the Lip and/or Palate in Xuzhou, China . Biomedical and Environmental Sciences, 2015, 28(7): 535-538. doi: 10.3967/bes2015.076
[13]	JIN Ling Ling, CHEN Er Jun, HOU Wei, LIU Xin Hua, HU Ying. The Association between Folate Pathway Genes and Cleft Lip With or Without Cleft Palate in a Chinese Population . Biomedical and Environmental Sciences, 2015, 28(2): 136-139. doi: 10.3967/bes2015.016
[14]	HUANG Gui Ling, WANG Shao Kang, SU Ming, WANG Ting Ting, CAI Hui Zhen, YIN Hong, SUN Gui Ju. Serum Folate, MTHFR C677T Polymorphism and Esophageal Squamous Cell Carcinoma Risk . Biomedical and Environmental Sciences, 2013, 26(12): 1008-1012. doi: 10.3967/bes2013.039
[15]	YUN WANG, XIN LI, WEN-LI ZHU, JIN-ZHEN GUO, XIAO-MING SONG, SHU-QIN LI, YONG LI. Genome-wide and Interaction Linkage Scan for Nonsyndromic Cleft Lip with or without Cleft Palate in Two Multiplex Families in Shenyang,China . Biomedical and Environmental Sciences, 2010, 23(5): 363-370.
[16]	LING WANG, JIE LI, YAN XIE, XUE-GUANG ZHANG. Association between Serum Homocysteine and Oxidative Stress in Elderly Patients with Obstructive Sleep Apnea/hypopnea Syndrome1 . Biomedical and Environmental Sciences, 2010, 23(1): 42-47.
[17]	LEI HUANG, XIAO-MING SONG, WEN-LI ZHU, YONG LI. Plasma Homocysteine and Gene Polymorphisms Associated with the Risk of Hyperlipidemia in Northern Chinese Subjects . Biomedical and Environmental Sciences, 2008, 21(6): 514-520.
[18]	WEI-YAN ZHAO, JIAN-FENG HUANG, LAI-YUAN WANG, HONG-FAN LI, PENG-HUA ZHANG, QI ZHAO, SHU-FENG CHEN, DONG-FENG GU. Association of the Apolipoprotein B Gene Polymorphisms With Essential Hypertension in Northern Chinese Han Population . Biomedical and Environmental Sciences, 2007, 20(3): 260-264.
[19]	XIAO-HONG ZHAO, GUANG JIA, YONG-QUAN LIU, SHAO-WEI LIU, LEI YAN, YU JIN, NIAN LIU. Association Between Polymorphisms of DNA Repair Gene XRCC1 and DNA Damage in Asbestos-Exposed Workers . Biomedical and Environmental Sciences, 2006, 19(3): 232-238.
[20]	JUN CHENG, WEN-LI ZHU, JING-JING DAO, SHU-QING LI, YONG LI. Relationship Between Polymorphism of Methylenetetrahydrofolate Dehydrogenase and Congenital Heart Defect . Biomedical and Environmental Sciences, 2005, 18(1): 58-64.

点击查看大图

计量

文章访问数: 1830
HTML全文浏览量: 521
PDF下载量: 65
被引次数: 0

BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

doi: 10.3967/0895-3988.2011.02.001

基金项目: the National Natural Science Foundation of China(307009907)%Beijing Natural Science Foundation(7082038)%Beijing Foundation for Excellent Elite(2010D003034000013)

刊出日期: 2011-04-20

关键词:

Nonsyndromic cleft lip with or without palate /

Homocysteine /

Folate /

Polymorphisms

摘要: Objective Convincing evidence suggests a link between increased risk of nonsyndromic cleft lip with or without cleft palate (NSCL/P) and low intake of folic acid by the mother during pregnancy. The present study was designed to explore if genetic variation in the betaine-homocysteine methyltransferase (BHMT) gene contributes to NSCL/P. Methods DNA was obtained from 166 individuals with NSCL/P and 285 healthy subjects. Three known single nucleotide polymorphisms (SNPs) present in the BHMT gene (rs651852, rs3797546, and rs3733890) were investigated by real-time PCR-based TaqMan genotyping. Results Neither allelic nor genotypic association was found between NSCL/P and SNPs rs651852 and rs3733890. SNP rs3797546 did not show allelic association with NSCL/P; however, a higher proportion of NSCL/P patients carry the CC genotype compared with the TT+CT genotype (P=0.020, OR=2.10, 95% Cl=1.11-3.95). Conclusion Our study suggests that polymorphism rs3797546 in the BHMT gene may confer genetic risk of NSCL/P in a recessive manner.

English Abstract

BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China

Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Funds: the National Natural Science Foundation of China(307009907)%Beijing Natural Science Foundation(7082038)%Beijing Foundation for Excellent Elite(2010D003034000013)

Publish Date: 2011-04-20

Keywords:

Nonsyndromic cleft lip with or without palate /
Homocysteine /
Folate /
Polymorphisms

Abstract: Objective Convincing evidence suggests a link between increased risk of nonsyndromic cleft lip with or without cleft palate (NSCL/P) and low intake of folic acid by the mother during pregnancy. The present study was designed to explore if genetic variation in the betaine-homocysteine methyltransferase (BHMT) gene contributes to NSCL/P. Methods DNA was obtained from 166 individuals with NSCL/P and 285 healthy subjects. Three known single nucleotide polymorphisms (SNPs) present in the BHMT gene (rs651852, rs3797546, and rs3733890) were investigated by real-time PCR-based TaqMan genotyping. Results Neither allelic nor genotypic association was found between NSCL/P and SNPs rs651852 and rs3733890. SNP rs3797546 did not show allelic association with NSCL/P; however, a higher proportion of NSCL/P patients carry the CC genotype compared with the TT+CT genotype (P=0.020, OR=2.10, 95% Cl=1.11-3.95). Conclusion Our study suggests that polymorphism rs3797546 in the BHMT gene may confer genetic risk of NSCL/P in a recessive manner.

Citation:

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816 / Q

Publish Under the Auspices of Chinese Center for Disease Control and Prevention

Distributed by Chinese Center for Disease Control and Prevention

Quick Links

Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net

doi: 10.3967/0895-3988.2011.02.001

BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

计量

出版历程

BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

doi: 10.3967/0895-3988.2011.02.001

English Abstract

BHMT Gene Polymorphisms as Risk Factors for Cleft Lip and Cleft Palate in a Chinese Population

Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

全文HTML

目录

Beijing Stomatological Hospital, Capital Medical University, Beijing 100050, China

Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China